PCRmax Mycoplasma species Instruction Manual

16S ribosomal RNA gene

Mycoplasma

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Mycoplasmas are a genus of Gram negative, aerobic, pathogenic bacteria that have been
isolated from many animal species. They are members of the class Mollicutes, which
contains approximately 200 known species.

They are unique among prokaryotes in that they lack a cell wall and are not susceptible to
many commonly prescribed antibiotics, including beta-lactams.

The small genome size of Mycoplasma organisms are associated with reduced metabolic
pathways such that they rely heavily on the nutrients afforded by the host environment.

N.B. genesig® has kits to detect specific types of mycoplasma and the handbooks for
these kits contain further details specific to that individual pathogen.

Introduction to Mycoplasma

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MIN

MAX

Among others, the kit will detect the following species of Mycoplasma: Mycoplasma
agassizii, Mycoplasma anatis, Mycoplasma anseris, Mycoplasma arginini,Mycoplasma
arthritidis, Mycoplasma auris, Mycoplasma buccale, Mycoplasma canadense,
Mycoplasma cloacale, Mycoplasma collis, Mycoplasma columborale, Mycoplasma
cricetuli, Mycoplasma cynos, Mycoplasma falconis,Mycoplasma faucium, Mycoplasma
felis, Mycoplasma gateae,Mycoplasma gypis, Mycoplasma hominis, Mycoplasma
hyopharyngis, Mycoplasma hyorhinis, Mycoplasma hyosynoviae, Mycoplasma iguana,
Mycoplasma lagogenitalium, Mycoplasma leonicaptivi, Mycoplasma molare, Mycoplasma
mustelae, Mycoplasma neophronis,Mycoplasma neurolyticum, Mycoplasma orale,
Mycoplasma phocicerebrale, Mycoplasma phocidae, Mycoplasma salivarium,
Mycoplasma spumans, Mycoplasma timone, and Mycoplasma zalophi

The PCR Max qPCR Kit for Mycoplasma (Mycoplasma) genomes is designed for the in
vitro quantification of Mycoplasma genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the Mycoplasma genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
Mycoplasma sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• Mycoplasma specific primer/probe mix (150 reactions BROWN)

FAM labelled

• Mycoplasma positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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