PCRmax Mycoplasma pneumoniae Instruction Manual

16S ribosomal RNA gene

Mycoplasma
pneumoniae

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Mycoplasma pneumoniae is a small bacterium of the Mycoplasmataceae family that infects
the mucosal membrane of the respiratory tract and causes tracheobronchitis and
pneumonia. The bacterium has a small genome of around 816k nucleotides arranged in a
double-stranded circular chromosome. This bacterium lacks a cell wall, this and the lack of
machinery for making essential organic compounds prevent it from living outside of a host
therefore making it a obligate, pathogenic species.

M. pneumoniae attaches to the epithelial cells of the respiratory tract using adhesion
proteins. Once attached to host cell it is thought that the bacterium may fuse with the
membrane of the host cell. After adhesion the bacterium produces hydrogen peroxide and
other oxide radicals that cause damage to the host cells. This in turn attracts neutrophils to
the infection site resulting in cytokine release and immune system activation.

The results of this bacterial infection cause symptoms that include coughing and fever.
Due to the lack of a cell wall in this species, antibiotics that target the bacterial cell wall are
not effective and so usual treatments include a course of erythromycin.

Introduction to Mycoplasma pneumoniae

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MIN

MAX

The PCR Max qPCR Kit for Mycoplasma pneumoniae (M.pneumoniae) genomes is
designed for the in vitro quantification of M.pneumoniae genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the M.pneumoniae
genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
M.pneumoniae sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• M.pneumoniae specific primer/probe mix (150 reactions BROWN)

FAM labelled

• M.pneumoniae positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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