PCRmax Mycoplasma hominis Instruction Manual
glyceraldehyde 3-phosphate
dehydrogenase (GAPDH) gene
Mycoplasma hominis
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Mycoplasma hominis is a bacterium of the Mycoplasma genus. Species within this genus
have small structural size with short genomes and lack the ability to perform many basic
metabolic functions. M. hominis lacks a cell wall and is only around 0.3µm in length with a
genome of around 800K nucleotides in length in a circular confirmation.
M. hominis may be part of the natural flora of the genitourinary tract but can also cause
infection in a wide range of tissues. This bacterium produces two membrane proteins that
act as adhesions to allow attachment to glycolipids of the genitourinary tract cells.
In child delivery, transmission is seen from the birth canal to the new born but studies have
shown that colonisation by this method is short lived, with transmission by sexual contact
being much more common. Colonisation with this bacterium has been linked to a number
of diseases including Pelvic Inflammatory Disease and post-abortum and post-partum
fever. Infections leading to infections including interstitial pneumonia and pharyngitis have
also been associated with M. hominis. M. hominis infections can be treated with antibiotics
including tetracyclin although resistance is emerging.
Introduction to Mycoplasma hominis
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MIN
MAX
The target sequence small within the glyceraldehyde 3-phosphate dehydrogenase gene,
has previously been shown to be a good genetic marker for M. hominis in other clinical
real-time PCR based studies (A. Baczynska et.al 2004). The primers and probe
sequences in this kit have 100% homology with a broad range of clinically relevant
reference sequences based on a comprehensive bioinformatics analysis.
The PCR Max qPCR Kit for Mycoplasma hominis (M.hominis) genomes is designed for the
in vitro quantification of M.hominis genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the M.hominis genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
M.hominis sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• M.hominis specific primer/probe mix (150 reactions BROWN)
FAM labelled
• M.hominis positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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