PCRmax Mycoplasma genitalium Instruction Manual
DNA gyrase subunit A (gyrA)
gene
Mycoplasma
genitalium
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
1
Mycoplasma genitalium is a small parasitic bacterium that lives on the ciliated epithelial
cells of the primate genital and respiratory tracts. M. genitalium is the smallest known
genome that can constitute a cell.
The genome of M. genitalium consists of 521 genes (~482 protein encoding genes) in one
circular chromosome of 582kbp.
Infection by M. genitalium seems fairly common, can be transmitted between partners
during unprotected coitus, and can be treated with antibiotics; however, the organism's role
in genital diseases is still unclear.
Symptoms include: Urethritis (men), Discharge, Burning while urinating, Arthritis/reactive
arthritis (mostly men), Vaginal itching (women),Pain during intercourse (women).This
infection is associated with bacterial vaginosis. In the long term, this infection is suspected
to cause pelvic inflammatory disease.
Introduction to Mycoplasma genitalium
2
MIN
MAX
The PCR Max qPCR Kit for Mycoplasma genitalium (M.genitalium) genomes is designed
for the in vitro quantification of M.genitalium genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the M.genitalium genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
M.genitalium sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• M.genitalium specific primer/probe mix (150 reactions BROWN)
FAM labelled
• M.genitalium positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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