PCRmax Mycoplasma felis Instruction Manual
Elongation factor Tu (tuf) gene
Mycoplasma felis
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Mycoplasmas are important Gram negative, aerobic, pathogenic microrganisms that have
been isolated from many animal species. They are members of the class Mollicutes, which
contains approximately 200 known species. Mycoplasma felis is a common parasite of cats
and usually located in the upper respiratory and lower genital tract. It can be carried
asymptomatically but can lead to conjunctivitis, respiratory disease and polyarthritis in cats,
as well as pneumonitis in horses.
M. felis can be isolated from the conjunctiva of both normal cats and those with
conjunctivitis. On the onset of the infection, unilateral conjunctivitis may be observed with
the opposite eye often becoming affected within 7-14 days. Early in the disease the
affected eye produces a serous discharge and may show signs of swelling of the
conjunctiva, hyperaemia and uncontrolled spasms or twitching of the eyelid. As infection
progresses the amount of discharge increases, becoming more mucoid, possibly
containing puss, and the conjunctival swelling becomes more severe. In some cases the
ocular discharge may adhere to the conjunctiva and appear as a pseudomembrane and
papillary hypertrophy of the conjunctiva may occur.
Introduction to Mycoplasma felis
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MIN
MAX
Our kit for Mycoplasma felis has been designed for the specific and exclusive in vitro
quantification of Mycoplasma felis. The primers and probe sequences in this kit have
100% homology with a broad range of clinically relevant reference sequences based on a
comprehensive bioinformatics analysis.
The PCR Max qPCR Kit for Mycoplasma felis (M.felis) genomes is designed for the in vitro
quantification of M.felis genomes. The kit is designed to have the broadest detection
profile possible whilst remaining specific to the M.felis genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
M.felis sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• M.felis specific primer/probe mix (150 reactions BROWN)
FAM labelled
• M.felis positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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