PCRmax Mycoplasma bovis Instruction Manual

DNA gyrase subunit B (gyrB)
gene

Mycoplasma bovis

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Mycoplasmas are small, wall-less bacteria which cause disease in all major species of
animals, including man. They are members of the class Mollicutes which also includes
acholeplasmas, ureaplasmas, hemoplasmas and spiroplasmas. There are more than 120
named Mycoplasma species. The majority of Mycoplasma species are host specific and
are not zoonotic.

In the UK Mycoplasma bovisis a major contributor to calf pneumonia and is the
mainMycoplasmaspecies identified from sick cattle in the UK.

However it is also associated with mastitis in cows, arthritis, meningitis, eye infections,
abortion and infertility.

Diagnosis at AHVLA is carried out by serological testing (egELISA) though the molecular
qPCR method is faster and as reliable.

Antibiotic studies onM. bovishave shown that many antibiotics are not effectivein
vitroand this includes the macrolides, lincosamides and tetracyclines, with some
fluoroquinolones becoming less effective. The results of thesein vitrotests is reflected in
the field situation whereM. bovisis difficult to treat.

An autogenousM. bovisvaccine appears to be effective though an isolate must be
obtained from the affected farm and the vaccine is made for use solely on that farm.

Introduction to Mycoplasma bovis

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MIN

MAX

The PCR Max qPCR Kit for Mycoplasma bovis (M.bovis) genomes is designed for the in
vitro quantification of M.bovis genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the M.bovis genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
M.bovis sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• M.bovis specific primer/probe mix (150 reactions BROWN)

FAM labelled

• M.bovis positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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