PCRmax Mycobacterium tuberculosis Instruction Manual

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Mycobacterium
Tuberculosis

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Mycobacterium tuberculosis is the bacterium that causes most cases of tuberculosis I

M. tuberculosis is an obligate aerobe (Gram positive mycobacterium).] M. tuberculosis
divides every 15 to 20 hours—extremely slowly compared to other bacteria, which tend to
have division times measured in minutes (for example, E. coli can divide roughly every 20
minutes). It is a small, rod-like bacillus that can withstand weak disinfectants and can
survive in a dry state for weeks but can grow only within a host organism.

Introduction to Mycobacterium Tuberculosis

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MIN

MAX

The primers have 100% homology with all other reference sequences in the NCBI
database.

Accession numbers for detected TB isolates:
CP000717.1, CP000611.1, AM408590.1, U43540.1, AE000516.2, BX842583.1,
BX842577.1, BX842572.1, BX248339.1, U35021.1, U35017.1, AF041819.1,
BX248346.1, BX248334.1,

The kit also detects strains of M. bovis including the M. bovis BCG strains

The PCR Max qPCR Kit for Mycobacterium Tuberculosis (TB) genomes is designed for
the in vitro quantification of TB genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the TB genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
TB sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• TB specific primer/probe mix (150 reactions BROWN)

FAM labelled

• TB positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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