PCRmax Moraxella catarrhalis Instruction Manual
major outer membrane protein
(copB) gene
Moraxella catarrhalis
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Moraxella catarrhalis is a Gram-negative bacterium of the Moraxellaceae family. M.
Catarrhalis is an aerobic bacterium that infects the upper respiratory tract and is known to
cause pneumonia and bronchitis. This bacterium is also known to be involved in
nosocomial infections.
M. catarrhalis can be transmitted via infected respiratory droplets and once inhaled, attach
to the epithelial cells of the respiratory tract via pili. Infection of the upper respiratory tract
can spread to the lower respiratory tract, usually occurring in individuals with underlying
respiratory problems such as Chronic Obstructive Pulmonary Disease (COPD) where it
can exacerbate the disease.
Antibiotics such as penicillin are only effective in the treatment of infections caused by
around 5% of M. catarrhalis strains as the remaining 95% of strains produce betalactamase. Therefore other antimicrobial therapies must be used to treat infections caused
by the vast majority of strains.
Introduction to Moraxella catarrhalis
2
MIN
MAX
The target sequence within the copB gene has previously been shown to be a good
genetic marker for M. catarrhalis in other clinical real-time PCR based studies (Greiner O,
et.al 2003). This gene has no known homology with other species in the Moraxella
genus. The primers and probe sequences in this kit have 100% homology with a broad
range of biologically relevant reference sequences based on a comprehensive
bioinformatics analysis.
The PCR Max qPCR Kit for Moraxella catarrhalis (M.catarrhalis) genomes is designed for
the in vitro quantification of M.catarrhalis genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the M.catarrhalis genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
M.catarrhalis sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
3
Kit Contents
• M.catarrhalis specific primer/probe mix (150 reactions BROWN)
FAM labelled
• M.catarrhalis positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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