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Mini Microplate Heat Sealer, Fixed Temperature
13204-11
Instructions for Use
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Mini Microplate Heat Sealer
Instructions for Use
2
Contents
1 Symbols Used in this Instruction Manual ......................................................... 3
2 Safety Precautions and Limitations of Use ...................................................... 4
3 Regulatory Limitations of Use .......................................................................... 8
4 Unit Description ............................................................................................... 9
5 Unit Installation .............................................................................................. 11
6 Unit Operation ................................................................................................ 13
6.1 Status Indicator LEDs .................................................................................... 13
6.2 Performing a Sealing Operation .................................................................... 14
7 Maintenance and Servicing............................................................................ 15
7.1 Replacing the Unit Fuse ................................................................................ 15
7.2 Over Temperature Safety Cut-out ................................................................. 16
7.3 Routine Cleaning and Inspection ................................................................... 16
7.4 Decontamination Procedure .......................................................................... 17
7.5 Transportation and Storage ........................................................................... 18
7.6 Product Disposal ............................................................................................ 18
8 Warranty and Returns .................................................................................... 19
9 Technical Specifications ................................................................................ 20
10 Glossary of Terms and Abbreviations ............................................................ 21
Tables
Table 1: Advisory Symbol Meanings ......................................................................... 3
Table 2: Unit Features ............................................................................................... 9
Table 3: Plate and Adapter Types ........................................................................... 10
Table 4: Recommended Film and Foil Types .......................................................... 10
Table 5: Mains Voltage Selection ............................................................................ 11
Table 6: Status Indicator LEDs ................................................................................ 13
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Mini Microplate Heat Sealer
Instructions for Use
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1 Symbols Used in this Instruction Manual
The following advisory symbols are used in this manual.
Table 1: Advisory Symbol Meanings
DANGER
Indicates a Risk of Electric Shock which could, if not avoided,
result in serve injury or death.
DANGER
Indicates a Burn Hazard which could, if not avoided, result in
serve injury or death.
DANGER
Indicates a Risk of Explosion which could, if not avoided,
result in serve injury or death.
WARNING
Indicates a hazardous situation which could, if not avoided,
result in serve injury or death; or severely damage the unit.
CAUTION
Indicates a hazardous situation which could, if not avoided,
result in minor or moderate injury; or degrade or impair the
functionality of the unit.
CAUTION
Indicates a Risk of Crush hazard due to moving parts which
could, if not avoided, result in minor or moderate injury.
Advisory note or other useful information.
NN
Refer to “section NN” for more details.
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Mini Microplate Heat Sealer
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2 Safety Precautions and Limitations of Use
It is essential that all users of this equipment have fully read and understood
the following safety precautions and limitations of use before installing or
operating the Mini Microplate Heat Sealer unit.
IMPORTANT
WARNING
The protection provided by this equipment may be impaired if
it is not used in a manner described in this manual.
WARNING
It is essential that the user of this equipment is aware of the
potential hazards associated with the unit and its accessories.
All operators should be familiar with the safety precautions
and warnings given in these instructions before attempting to
operate the unit.
Improper use of this unit or its accessories may impair their
functionality and invalidate the manufacturer’s warranty.
Unit Handling Precautions
CAUTION
Care should be taken not to drop the unit or subject it to rough
physical handling, both during normal use and during
transportation and storage.
WARNING
The unit should be held by the handle when being lifted or
moved. Do not lift the unit by any other part of the casework.
Care should be taken when lifting due to the unit’s weight.
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Unit Installation and Operating Environment
DANGER
WARNING
The Mini Microplate Heat Sealer unit is designed for indoor
laboratory use only.
The acceptable operating temperature range is 18ºC to 30ºC,
with a relative humidity of 20% to 80% non-condensing, at a
maximum altitude of 2200m above sea level.
If the unit is stored in conditions outside of these ranges, it
must be left to stand unpowered until it has acclimatised to
within these environmental limits before being powered.
DANGER
Use only the AC mains power cord provided with the unit or as
specified in section 9.
The unit must be connected to a suitably earthed mains
supply, with appropriate earth-leakage and over-current
protection.
WARNING
Always ensure that the mains power connector is securely
inserted into the rear of the unit, and any excess power cord
does not pose a potential trip or pull hazard.
DANGER
Do not operate the unit in any area which is, or has been, or is
thought to have been exposed to explosive or flammable
gases, vapours or liquids.
WARNING
The unit must be installed and operated on a solid, stable and
level working surface.
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General Operating Precautions
DANGER
Ensure that the power is switched off at both the AC mains
supply outlet and at the back of the unit before inserting or
removing the mains power cord.
DANGER
The heater plate reaches a temperature of 170ºC and will
remain hot for a considerable time after being turned off.
Extreme care must be taken not to touch the heater plate as it
will cause a serve burn injury.
DANGER
The unit is intended for use with plates containing biological
samples only.
Never use the unit to seal any explosive, volatile or highly
reactive substances or chemicals.
CAUTION
There is a possible finger crush hazard due to the moving
parts of the handle and plate carrier.
Care should be taken when operating the handle.
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Unit Maintenance and Serviceability
DANGER
WARNING
There are no user or operator serviceable parts inside the
unit.
Do not remove the unit casework.
Removal of the unit's casework will void the manufacturer’s
warranty and may expose the user to a Risk of Electric Shock
resulting in serious injury or death.
DANGER
Once installed, the externally accessible unit fuse will only
blow under a fault condition. This fuse should only be changed
after the unit has been thoroughly inspected by a qualified
engineer. See section 7.1 for details.
DANGER
Always switch off the unit and disconnect the power cord
before performing any cleaning or decontamination procedure.
If liquid is spilt into or over the unit, switch off and disconnect
the power from the AC mains outlet before attempting to deal
with the spillage.
CAUTION
Ensure that the heater plate has cooled down to room
temperature before performing any cleaning operation and
before moving or storing the unit.
CAUTION
The use of harsh chemicals and cleaning agents may damage
the unit and degrade its performance.
Always follow the cleaning and decontamination procedures
specified in sections 7.3 and 0 of this instruction manual.
CAUTION
Do not autoclave any part of the unit or its accessories.
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Mini Microplate Heat Sealer
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3 Regulatory Limitations of Use
Declaration of Conformity
This product fulfils the essential requirements of the Low
Voltage Directive (LVD) 2006/95/EC and the EMC Directive
2004/108/EC, when installed and operated in accordance with
the instructions in this manual.
The Mini Microplate Heat Sealer unit has been type tested by
Trac Global (UKAS approved test facility and UK appointed
Notified Body) against the EMC Requirements listed below,
and issued Certificate No 6660/10 and 6661/10.
Safety and EMC Requirements
SAFETY
• EN 61010-1:2010, EN 61010-2-051:2003
• UL 61010-1:2001 2nd Edition (CAN C22.2 CSA 61010-1)
EMC
• EN 61326:2006, Class B
• FCC CFR 47 Parts 15.107 and 15.109, Class B
WEEE Directive Compliance
WEEE
Where applicable, the Mini Microplate Heat Sealer unit should
be disposed of in accordance with the European Union WEEE
Directive 2002/96/EC on Waste Electrical and Electronic
Equipment.
Do not dispose of this product into unsorted municipal waste
or public landfill. Please refer to section 7.6 for details of how
to correctly dispose of this product.
The Mini Microplate Heat Sealer unit is designed and manufactured under ISO 9001 by:
Integrated Technologies Limited
Viking House, Ellingham Way, Ashford, Kent, TN23 6NF
United Kingdom
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4 Unit Description
The Mini Microplate Heat Sealer fixed temperature sealer provides a safe
and controlled method for sealing plastic sample plates to protect the
samples from evaporation and contamination during storage.
The unit has the following external features:
Table 2: Unit Features
Seal Activation Handle
6.2
Status Indicator LEDs
6.1
Heater Plate (Internal)
6.2
Adapter Plate Carrier
Table 3
Power Inlet, On/Off Switch and Fuse Holder
5
Mains Voltage Selector
5
Product Information Label
9
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The Mini Microplate Heat Sealer unit is designed to be used with a range of
sample plates and consumables – some of which are listed below:
Table 3: Plate and Adapter Types
Adapter Type
Suitable Plate Types
Product Number 13204-13
96 Well Microtiter Plate
384 Well Microtiter Plate
PCR 96 Well Skirted Plate
Product Number 13204-15
PCR 96 Well Semi-skirted Plate
PCR 96 Well Unskirted Plate
Product Number 13204-17
Deep-well Plates
Plate Material Types
Polypropylene, Polyethylene or Polystyrene
Other specialty adapter plates may be available on request. Please contact
Cole-Parmer for details.
Table 4: Recommended Film and Foil Types
Films
Clear Polyester/Polypropylene Laminate
Clear Polymer
Thin Clear Polymer
Foils
Foil Polypropylene Laminate
Foil Laminate
Foil
A comprehensive range of clear films and foils are available. Please contact
Cole-Parmer for details.
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Mini Microplate Heat Sealer
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5 Unit Installation
Before installing the Mini Microplate Heat Sealer unit, please check that the
delivery is complete and that the unit and any accessory parts are intact and
free from any signs of transportation damage. Also, ensure that all external
and internal packaging has been removed from the unit before installation.
Please retain all packaging for future transportation and storage of
the unit and its accessories.
The Mini Microplate Heat Sealer unit should be installed in a location which
meets the following requirements:
• Safe and suitable operating environment (see section 2)
• Solid, stable, level working surface
• At least 10cm clearance around the unit to adjacent objects and walls
• Earthed AC mains power connection (see section 9)
WARNING
Please also observe and abide by the Unit Installation and
Operating Environment safety precautions and preconditions
listed in section 2.
It is important the check that the Mains Voltage Selector switch on the rear of
the unit has been set to the correct position for the desired operating voltage,
and the correct value of fuse has been fitted (see Table 5).
Table 5: Mains Voltage Selection
Operating Voltage
110 to 120 VAC
220 to 230 VAC
Voltage Selection
See Section 4 for
switch location ()
on unit rear panel.
115
230
Fuse Rating
3.15AH
1.6AH
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WARNING
Ensure that the correct fuse type has been fitted in the mains
inlet fuse holder for the desired voltage selector switch value.
See Table 5 for details.
▼
▲
1. Remove the AC plug from the power inlet module
2. Use a small flat bladed screwdriver to push the two
securing tabs inwards (highlighted in red) and pull
out the fuse holder
3. Fit the correct fuse in the lower position only
4. Push fuse holder back into mains inlet module
Install and test the Mini Microplate Heat Sealer unit using the following
procedure:
1) Place the unit on the suitably selected working surface (as specified
above).
2) Connect the unit to the AC mains power outlet using the mains
power cord supplied or as specified in section 9.
3) Switch the mains power on at supply outlet first, and then switch the
unit on using the power switch located at the rear of the unit.
4) Check that the unit is stable and safe by performing a trial sealing
operation, as described in section 6.2.
DANGER
If the unit has been stored in a cool environment, it must be
left to stand unplugged until it has acclimatized to the new
room temperature before being powered.
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6 Unit Operation
WARNING
Please ensure that you have read and fully understood all the
Safety Precautions and Limitations of Use listed in section
2 before attempting to operate the Mini Microplate Heat Sealer
unit.
6.1 Status Indicator LEDs
The Mini Microplate Heat Sealer unit operators at a fixed sealing temperature
and requires no user set-up or controls. Hence, there are just two status
indicator LEDs on the front of the unit with the functions listed in Table 6.
Table 6: Status Indicator LEDs
Amber Heater Indicator
On or Off – Heater Plate is warming up
Flashing – Heater Plate at 170ºC ±2ºC
Green Power Indicator
On – Unit switched on
Off – Unit switched off (or no power)
When the unit is switched on, the green Power LED will illuminate and the
unit will automatically start heating to 170ºC. This is indicated by the amber
Heater LED being on.
Once the heater plate has reached the correct temperature to perform
sealing (within 2ºC of the 170ºC set-point) the Heater LED will start flashing,
and the Mini Microplate Heat Sealer is ready for use.
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6.2 Performing a Sealing Operation
Various types of plastic sample plates may be loaded onto a plate carrier and
thermal sealed using a suitable foil or clear film. See section 4 for details.
A typical operating sequence is:
1) Switch the Mini Microplate Heat Sealer on
at the rear of the unit and allow sufficient
time for it to reach temperature (the
Heater LED will start flashing).
2) Using a suitable adapter plate (listed in
Table 3), load the sample plate onto the
plate carrier and add the sealing film on
top, the correct way up.
WARNING
Care should be taken not to touch the
surface of the heater plate whilst
loading the sample plate.
3) Grasp the handle with one or two hands,
as appropriate, and pull the handle
forward and down until the heater plate
contacts the sample plate.
CAUTION
Do not to apply more force to the
handle than is necessary to keep the
heater in contact with the sample.
4) When the required amount of time has
passed, return the handle to its upmost.
CAUTION
Leaving the handle down for too long
could damage the sample plate and
degrade the seal quality.
5) Remove the sealed plate from the unit and
review the seal integrity.
WARNING
The sample plate and film/foil may
remain hot for several seconds after
being removed from the unit.
The time taken to successfully seal a sample plate may vary with both plate
and film/foil type. For optimum seal integrity, the sealing time may need to be
adjusted empirically to achieve the desire seal quality.
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Instructions for Use
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7 Maintenance and Servicing
Although the Mini Microplate Heat Sealer unit does not require scheduled
maintenance or servicing, the operator should regularly clean and inspect the
unit for any detects, as described in section 7.3 below.
DANGER
WARNING
Please observe and comply with all the Unit Maintenance
and Serviceability precautions listed in section 2.
Never removed the unit casework. There are no user or
operator serviceable parts inside the unit.
Always switch off and unplug the unit before performing any
cleaning or disinfecting tasks.
For technical and service related enquiries, please contact Cole-Parmer.
7.1 Replacing the Unit Fuse
The unit fuse should only be replaced by a suitably qualified technician.
DANGER
The unit fuse will only blow as a result of an internal unit fault
or if the voltage selector switch has been incorrectly set (see
section 5). This fuse should only be changed after the unit has
been thoroughly inspected, and must be replaced with the
exact type specified in section 9.
Thoroughly inspect the unit for any signs of damage, loose components or
liquid spillage or ingress. If in doubt, please contact Cole-Parmer.
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DANGER
The fuse holder is removed by disconnecting the mains cord
and then using a small flat bladed screwdriver to carefully
unclip and pull open the fuse holder (see section 5).
After replacing the fuse with the corrected rated one for the
operating voltage being used (see Table 5), push the fuse
holder firmly back into the inlet module.
The unit must be electrically safety tested for excess leakage
current before being repowered from the mains supply.
7.2 Overtemperature Safety Cut-out
The unit is fitted with a non-resettable thermal fuse which blows if the heater
plate temperature exceeds 195 ºC.
In the unlikely event of a fault condition, this fuse will permanently disable the
heater to protect the user and unit from injury and damage. If this occurs, the
Heater indicator will light but heater plate will remain cold and the Mini
Microplate Heat Sealer will need to be returned for repair.
7.3 Routine Cleaning and Inspection
The unit casework should be cleaned and inspected at regular intervals, and
whenever contamination or spillage occurs, as follows:
1. Switch off the unit and disconnect the power before performing any
inspection checks or cleaning.
2. Before cleaning, always inspect the unit casework, heater plate and
moving parts for any signs of wear, damage, cracks or other defects.
3. Clean the casework using a damp cloth soaked with a disinfectant
solution (such as Virkon), whilst wearing suitable PPE.
4. Carefully clean the heater plate surface to remove any debris or
sealing material.
5. Remove any debris or fluff from around or between the moving parts
of the handle mechanism.
6. Check and clean the adapter blocks.
DANGER
After cleaning, ensure that the unit is thoroughly dry,
especially around the mains power inlet, before reconnecting
the power cord and switching the unit on.
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7.4 Decontamination Procedure
The unit and accessories should be decontaminated using the following
procedure before being stored or transported.
Certificate of Decontamination
We respect the health and safety of our clients and employees, and request
that any products or accessories being returned are decontaminated in
accordance with the procedure below.
1. Decontamination Procedure
Thoroughly clean all outside surfaces of the product (including any
accessories, power cords, manuals, packaging, etc) with a damp cloth
soaked with suitable disinfectant solution (such as Virkon).
Allow to dry fully before packing.
2. Decontamination Declaration
Company Name:
Address:
Product Code:
13204-11
Serial Number:
Reason for Return:
Where Product Used:
Please tick the appropriate option(s) below:
I certify that I have decontaminated the product as per the above procedure.
Decontaminant Used:
I certify that the product has not been exposed to any chemical or biological materials.
Title:
Name:
Signature:
Date:
Telephone:
Email:
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7.5 Transportation and Storage
The Mini Microplate Heat Sealer unit and its accessories should be
thoroughly decontaminated using the procedure detailed in section 0 before
being placed in its original packaging for transportation or storage.
WARNING
Refer to section 9 for the acceptable range of Storage and
Transportation environmental conditions.
Always ensure that the unit and accessories are completely
dry and free of any condensation before being packed.
7.6 Product Disposal
At end-of-life, this product must be disposed of in accordance with your local
authority regulations for the disposal of potentially hazardous waste and
electronic equipment.
The unit and its accessories should be decontaminated using the procedure
detailed in section 0 before disposal or shipping.
Do not dispose of this product into unsorted municipal waste
or public landfill.
Please contact Cole-Parmer for details of how to correctly dispose of this
product.
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Mini Microplate Heat Sealer
Instructions for Use
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8 Warranty and Returns
Cole-Parmer warrants the Mini Microplate Heat Sealer product, when
purchased new and installed and operated in accordance with the
instructions of this manual, to be free from defects in materials and
workmanship, and will repair or replace, at their discretion, any unit or
accessory which exhibits such defects.
In no event will Cole-Parmer be liable for any indirect, incidental or
consequential damages resulting from any defect or warranty claim.
NOTE
Unspecified use or unauthorized modification of any part of the
Mini Microplate Heat Sealer unit or its accessories or the use or
attachment of any adapter or peripheral not supplied, specified or
sanctioned by Cole-Parmer will invalidate this warranty.
This warranty is provided to the original purchaser of the product for one year
from the date of purchase.
Under the terms of this warranty, the product must be returned in its original
packaging, transportation prepaid by the sender, with a copy of the Proof of
Purchase and a detailed description of the problem.
WARNING
The product must be decontaminated using the procedure
detailed in section 0 and a Certificate of Decontamination
supplied with any return. If the product is considered too
hazardous to be shipped, please contact Cole-Parmer for
further instructions.
Please contact Cole-Parmer to receive authorization to return the product.
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Mini Microplate Heat Sealer
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9 Technical Specifications
Product Information
Name
Product Number
Mini Microplate Heat Sealer
13204-11
Accessories
Adapter plate, microtiter
Adapter plate, 96 well
Adapter plate, deep well
13204-13
13204-15
13204-17
Physical Unit Properties
Dimensions (W x D x H)
Weight (without adapter)
220 mm x 250 mm x 230 mm
4 kg
Mains Supply
Power Cord Rating
Inlet Module Type
Supply Voltage Selections
Supply Frequency Range
Power Consumption
Fuse Ratings and Sizes
IEC C13, 3-Core, 5A min
IEC C14, DPST, Single Fuse
115 110 to 120 VAC ±10%
230 220 to 230 VAC ±10%
50 to 60 Hz ±5%
350 W max
115 T3.15AH 250V 20x5mm
230 T1.6AH 250V 20x5mm
Operating Environment
Temperature Range
Relative Humidity Range
Maximum Operating Altitude
+18 to +30 C
20% to 80% non-condensing
2200 m above sea-level
Storage and Transportation
Temperature Range
Relative Humidity Range
-10 to +50 C
20% to 95% non-condensing
Sealing Parameters
Temperature Set-point
Temperature Accuracy
170 C (Non-adjustable)
±2 C
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10 Glossary of Terms and Abbreviations
ANSI
American National Standards Institute
Deep-well Plate
Plate with an SBS footprint featuring 48, 96 or 384
wells with a larger volume than microplates
DWP
Deep-well plate
EMC
Electro-Magnetic Compatibility
Microtiter Plate
Plate with an SBS footprint featuring 24, 48, 96 or
384 wells
MTP
Microtiter plate
PCR
Polymerase Chain Reaction
PPE
Personal Protective Equipment
SBS
Society for Bio Molecular Screening
Semi-skirted PCR
Plate
PCR plate with an outer surrounding half edge
Skirted PCR Plate
PCR plate with an outer surrounding edge
Unskirted PCR Plate
PCR plate without an outer surrounding edge
Well
A single sample cavity in a Microtiter plate, PCR
plate or Deep-well plate
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Instructions for Use
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In the US
625 E. Bunker Court
Vernon Hills, IL 60061
Call toll-free 1-800-323-4340
Phone: 1-847-549-7600
Fax: 1-847-247-2929
www.coleparmer.com
In Canada
Call toll-free 1-800-363-5900
Phone: 1-514-355-6100
Fax: 1-514-355-7119
www.coleparmer.ca
In the United Kingdom
Free phone: 0500-345-300
Phone: 020-8574-7556
Fax: 020-8574-7543
www.coleparmer.co.uk
In India
Phone: 91-22-6716-2222
Fax: 91-22-6716-2211
www.coleparmer.in
International customers
Call 1-847-549-7600 to reach
our International Sales
Department or contact your
local dealer
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