PCRmax Microsporum gypseum Instruction Manual

Beta-tubulin gene

Microsporum gypseum

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Microsporum gypseum is a geophilic fungus with a world-wide distribution and it may
cause infections in animals and humans, particularly children and rural workers during
warm humid weather. They belong to the genus Microsporum that causes tinea capitis,
tinea corpus, ringworm, and other dermatophytoses (fungal infections of the skin).
Microsporum forms both macroconidia (large asexual reproductive structures) and
microconidia (smaller asexual reproductive structures) on short conidiophores.
Macroconidia are hyaline, multiseptate, variable in form, fusiform, spindle-shaped to
obovate, 7–20 by 30–160 um in size, with thin or thick echinulate to verrucose cell walls.
Their shape, size and cell wall features are important characteristics for species
identification. They are hyaline, single-celled, pyriform to clavate, smooth-walled, 2.5–3.5
by 4–7 um in size and are not diagnostic for any one species.

Introduction to Microsporum gypseum

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MIN

MAX

The PCR Max qPCR Kit for Microsporum gypseum (M.gypseum) genomes is designed for
the in vitro quantification of M.gypseum genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the M.gypseum genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
M.gypseum sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• M.gypseum specific primer/probe mix (150 reactions BROWN)

FAM labelled

• M.gypseum positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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