PCRmax Lyophilised OneStep 2X RT-qPCR Master Mix Instructions for use

PCRMaxTM

Lyophilised OneStep
2X RT-qPCR Master Mix

Instructions for use of Lyophilised OneStep Master Mix

Lyophilised OneStep 2X RT-qPCR Master Mix Handbook HB04.04.05

Published Date: 17 Nov 2017

Contents

Introduction 3

Kit contents 4

Kit storage 4

Suitable sample material 4

Licensing agreement and limitations of use 4

Resuspension protocol 5

OneStep RT-qPCR detection protocol 6

OneStep RT-qPCR amplification protocol 7

Lyophilised OneStep 2X RT-qPCR Master Mix Handbook HB04.04.05

Published Date: 17 Nov 2017

2

Introduction

Our Lyophilised OneStep 2X RT-qPCR Master Mix is an optimised complete system
for use in OneStep real-time PCR. Removal of a separate Reverse Transcription step,
reduces handling errors, and greatly reduces the time taken to obtain results. The
product is stable at ambient temperatures for at least 18 months and can be
conveniently shipped and stored at room temperature. The master mix contains a
thermo-stable TAQ Polymerase and MMLV as well as buffer, dNTPs, MgCl2 and
stabilisers at concentrations optimised for the enzymes. Once resuspended, only the
template RNA and primer and probe mix are required to complete the experimental set
up for a perfect single tube reaction. The master mix has been freeze-dried to produce
a room temperature stable preparation.

The kit includes the lyophilised master mix, resuspension buffer and a tube of ROX dye
which can be added as required when the master mix is to be used on hardware
platforms that use ROX as a passive reference dye.

Lyophilised OneStep 2X RT-qPCR Master Mix Handbook HB04.04.05

Published Date: 17 Nov 2017

3