PCRmax Legionella longbeachae Instruction Manual

DNA gyrase subunit B (gyrB)
gene

Legionella
longbeachae

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

1

Legionella is a genus of Gram-negative bacterium. This genus contains over 50 species, of
which there are 2 which cause pathogenesis in humans; these being L. pneumophila and
L. longbeachae. All of these bacteria can cause Legionellosis, which can be a relatively
common cause of nosocomial and community acquired pneumonia.

Legionella longbeachae is one species of the family Legionellaceae. It was first isolated
from a patient in California in the United States. It is found mainly in soil and contaminated
potting compost. In humans, the infection can be known as ‘Pontiac Fever.’ This
bacterium is thought to cause pneumonia in people with diseases of the immune system.

The genome organization and gene content of the four L. longbeachae genomes are highly
conserved, indicating strong pressure for niche adaptation

Human infection from L. longbeachaeis particularly common in Australia but cases have
been documented in other countries including the United States, Japan, Greece and the
UK.

The infection can be very serious, often leading to hospitalisation and sometimes death.
Like other Legionella species, person-to-person transmission has not been documented.
However, unlike other species the primary transmission mode is inhalation of dust from
contaminated compost or soil that contains the organism causing legionellosis.

Modes of transmission include poor hand-washing practices after gardening, long-term
smoking and being near dripping hanging flower pots. Exposure to aerosolized organisms
and poor gardening hygiene may be important predisposing factors to L. longbeachae
infection.

If exposure is a concern, contaminated compost must be handled with care, dampened
with water to reduce dust before handling and utilisation of a face mask to reduce the risk
of inhaling the dust.

Introduction to Legionella longbeachae

2

MIN

MAX

The PCR Max qPCR Kit for Legionella longbeachae (L.longbeachae) genomes is
designed for the in vitro quantification of L.longbeachae genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the L.longbeachae

genome.

The primers and probe sequences in this kit have 100% homology with a broad range of L.
longbeachae sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

3

Kit Contents

• L.longbeachae specific primer/probe mix (150 reactions BROWN)

FAM labelled

• L.longbeachae positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

4