PCRmax Lactobacillus plantarum Instruction Manual

Plantaricin F (plnF) gene

Lactobacillus
plantarum and
Lactobacillus
paraplantarum

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

1

Lactobacillus plantarum (L. plantarum) is a gram positive bacterium that is found in the
human gastrointestinal tract. It is a lactic acid bacterium that utilizes an extensive range of
fermentatable carbon sources. It is a non-spore forming, fermentative bacteria that grows
anaerobically. L. plantarum has been identified as a probiotic and as such can have a
positive impact on human health, especially to those who have gastrointestinal problems.
The cells are small (0.5 – 1.2 x 1.0 – 10μm), elongated rod-like bacilli assembled in pairs
or in chains of variable length.

L. plantarum has one of the largest genomes among lactic acid bacteria containing
3,308,274 base pairs arranged in a circular chromosome with approximately 2100 genes
putatively identified. The average GC content is 45%. The genome and plasmids are
geared to ensure adaptability to a wide range of environmental conditions. It can grow in­between 15-45 °C and pH levels of 3.2 and higher. This versatility allows L. plantarum
isolates to be found in human saliva, fermenting dairy products, plant material and silage.

Because of its ability to inhibit growth of harmful bacteria, L. plantarum is marketed as a
probiotic and benefits may include an improvement of microbe balance, stabilization of
digestive enzyme patterns, and immunomodulation by activating and regulating mucosa­associated and systemic immune system responses. Due to its facultative hetero­fermenter nature and its ability to digest a wide range of plant substrates, it is also being
explored as a possible producer of biofuels and bioproducts.

Introduction to Lactobacillus plantarum and
Lactobacillus paraplantarum

2

MIN

MAX

Due to the similarities between L.plantarum and L.paraplantarum, the kit will detect both
species.

The PCR Max qPCR Kit for Lactobacillus plantarum and Lactobacillus paraplantarum (L.
plantarum/L.paraplantarum) genomes is designed for the in vitro quantification of L.
plantarum/L.paraplantarum genomes. The kit is designed to have the broadest detection
profile possible whilst remaining specific to the L.plantarum/L.paraplantarum genome.

The primers and probe sequences in this kit have 100% homology with a broad range of L.
plantarum/L.paraplantarum sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

3

Kit Contents

• L.plantarum/L.paraplantarum specific primer/probe mix (150 reactions BROWN)

FAM labelled

• L.plantarum/L.paraplantarum positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

4