PCRmax Klebsiella oxytoca Instruction Manual

Outer membrane protein II
(ompA) gene

Klebsiella oxytoca

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Klebsiella oxytoca is a Gram-negative, rod-shaped bacterium that is closely related to K.
pneumonia and differs in that it is indole-positive and able to grow on melezitose, but not 3­hydroxybutyrate. Klebsiella oxytoca has increasingly been present in the blood samples of
infants suffering from neonatal septicemia. The bacterium has a cylindrical rod shape
measuring 2 µm by 5µm with a genome that ranges in size from 5.28-5.97Mbp with certain
strains lacking a polysaccharide capsule.

In humans, this species tends to colonize along the mucosa membranes of the colon and
nasopharynx, and skin; however, they can be found colonizing on all parts of the body.
Most infections of K. oxytoca are nosocomial, spreading via the hands of medical staff.
Outbreaks occur in patients with immunodeficient diseases and patients who are being
treated with antibiotics. They have the ability to colonize many different areas of the human
body such as the skin, GI tract, sterile wounds, urine, and skin. The bacteria overcome
innate host immunity through several means. They possess a polysaccharide capsule,
which is the main determinant of their pathogenicity. The capsule is composed of complex
acidic polysaccharides. Its massive layer protects the bacterium from phagocytosis by
polymorphonuclear granulocytes. In addition, the capsule prevents bacterial death caused
by bactericidal serum factors. The bacteria also produce multiple adhesins. These may be
fimbrial or nonfimbrial, each with distinct receptor specificity. These help the
microorganism to adhere to host cells, which is critical to the infectious process.

Symptoms of neonatal septicemia include seizures, slow heart rate, temperature changes,
jaundice, vomiting, diarrhea, low blood sugar, breaking difficulties, reduction in movements
and sucking, and swollen abdomen. Urinary Tract Infections (Cystitis) produces symptoms
of burning or pain on urination and a frequent need to urinate even if the bladder is not full.
A bladder infection that worsens may spread to the kidneys, leading to a more serious
condition called pyelonephritis with symptoms that may include lower back pain, nausea
and vomiting. Gastrointestinal infection with Klebsiella oxytoca may also cause severe
cases of colitis that produce gastrointestinal upset and bloody diarrhea. Klebsiella oxytoca
may also cause Respiratory infections such as pneumonia. Symptoms include coughing,
chest pain, fever, shortness of breath and general weakness. The bacteria is also capable
of causing septicemia, which is infection of the blood and allows the bacteria to spread to
other organs.

Introduction to Klebsiella oxytoca

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MIN

MAX

The PCR Max qPCR Kit for Klebsiella oxytoca (K.oxytoca) genomes is designed for the in
vitro quantification of K.oxytoca genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the K.oxytoca genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
K.oxytoca sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• K.oxytoca specific primer/probe mix (150 reactions BROWN)

FAM labelled

• K.oxytoca positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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