PCRmax Herpes simplex type 1 (HHV1) Instruction Manual

Capsid assembly and DNA
maturation gene

Human Herpes Virus 1
(Herpes simplex type 1)

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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The herpes simplex virus (HSV) (also known as Cold Sore, Night Fever, or Fever Blister) is
a virus that manifests itself in two common viral infections (HSV1 and HSV 2), each
marked by painful, watery blisters in the skin or mucous membranes (such as the mouth or
lips) or on the genitals. The disease is contagious, particularly during an outbreak, and is
incurable with present technology. An infection on the lips is commonly known as a "cold
sore" or "fever blister". These are sometimes confused with canker sores or Apthous ulcers
which have a similar appearance; these appear inside the mouth and are not caused by
the herpes simplex virus.

Introduction to Human Herpes Virus 1 (Herpes
simplex type 1)

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MIN

MAX

Sequence data is limited. However the primers have 100% homology with the reference
sequence in the NCBI database. The primers have low homology to the HSV-2
sequences in this regions and the kit therefore has a highly specific quantification profile.
It is not possible guarantee the quantification of all clinical isolates.

The PCR Max qPCR Kit for Human Herpes Virus 1 (Herpes simplex type 1) (HSV1)
genomes is designed for the in vitro quantification of HSV1 genomes. The kit is designed
to have the broadest detection profile possible whilst remaining specific to the HSV1
genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
HSV1 sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• HSV1 specific primer/probe mix (150 reactions BROWN)

FAM labelled

• HSV1 positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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