PCRmax Herpes simplex type 1 and 2 (HHV1&2) Instruction Manual
DNA polymerase (UL30) gene
Herpes Simplex Virus 1
and 2
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
1
The herpes simplex virus (HSV) is a virus that manifests itself in two common viral
infections (HSV1 and HSV 2), each marked by painful, watery blisters on the skin, mucous
membranes (such as the mouth or lips) or on the genitals. The double-stranded DNA virus
belongs to the family Herpesviridae, subfamily Alphaherpesviridae and has a circular
genome of 155 kb. HSV-1 and HSV-2 share the same overall structure, as well as very
high similarity in most genes. Both HSV-1 and HSV-2 have two unique regions, named UL
and US surrounded and defined by three sets of inverted repeats, named LTRa, LTRb and
LTRc. The UL and US regions are found in both forward and inverted directions and wild
type HSV occurs equally in all four possible arrangements (isomers) of the UL and US
regions. The coordinate system is defined to begin at the first nucleotide of the shortest
terminal repeat, LTRa, followed by LTRb, and the long unique region, UL.
The disease is contagious, particularly during an outbreak, and is currently incurable. HSV
-1 and HSV-2 are closely related but differ in epidemiology. HSV-1 is traditionally
associated with facial disease while HSV-2 is traditionally associated with genital disease.
HSV is transmitted by close personal contact, and infection occurs via inoculation of virus
into susceptible mucosal surfaces (eg, oropharynx, cervix, conjunctiva) or through small
cracks in the skin. The virus is readily inactivated at room temperature and by drying;
hence, aerosol and fomitic spread are rare.
The clinical course of herpes simplex infection depends on the age and immune status of
the host, the anatomic site of involvement, and the antigenic virus type. Primary herpes
simplex virus HSV–1 and HSV-2 infections are accompanied by systemic signs, longer
duration of symptoms, and higher rate of complications. Recurrent episodes are milder and
shorter. Both HSV-1 and HSV-2 can cause similar genital and orofacial primary infections
after contact with infectious secretions containing the virus or can be asympomatic.
Symptoms of genital herpes may include itching or tingling sensations in the genitals,small
fluid-filled blisters that burst leaving sores, headaches, backache and flu-like symptoms,
including swollen glands or fever.
Introduction to Herpes Simplex Virus 1 and 2
2
MIN
MAX
The PCR Max qPCR Kit for Herpes Simplex Virus 1 and 2 (HSV1&2) genomes is
designed for the in vitro quantification of HSV1&2 genomes. The kit is designed to have
the broadest detection profile possible whilst remaining specific to the HSV1&2 genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
HSV1&2 sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• HSV1&2 specific primer/probe mix (150 reactions BROWN)
FAM labelled
• HSV1&2 positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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