PCRmax Helicobacter pylori Instruction Manual

RNA polymerase beta-subunit
(rpoB) gene

Helicobacter Pylori

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Helicobacter pylori (previously named Campylobacter pylori) is Gram-negative,
microaerophilic a bacterium that infects the mucus lining of the stomach and duodenum.
Many cases of peptic ulcers, gastritis, and duodenitis are caused by H. pylori infection.
However, many who are infected do not show any symptoms of disease. Helicobacter
bacteria are the only known microorganisms that can thrive in the highly acidic
environment of the stomach. Its helical shape (hence the name helicobacter) is thought to
have evolved to penetrate and colonize the mucus lining.
It is also linked to the development of duodenal ulcers and stomach cancer. However, over
80 percent of individuals infected with the bacterium are asymptomatic and it has been
postulated that it may play an important role in the natural stomach ecology
Infection with H. pylorican can be confirmed by a test done on a sample of faeces (stool) or
in a breath test, or from a blood test, or from a biopsy sample taken during an endoscopy.

Introduction to Helicobacter Pylori

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MIN

MAX

This kit will detect all Helicobacter pylori strains.

The PCR Max qPCR Kit for Helicobacter Pylori (H.pylori) genomes is designed for the in
vitro quantification of H.pylori genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the H.pylori genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
H.pylori sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• H.pylori specific primer/probe mix (150 reactions BROWN)

FAM labelled

• H.pylori positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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