PCRmax Geosmithia argillacea Instruction Manual

Calmodulin (Cmd) gene

Geosmithia argillacea

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Geosmithia argillacea is a filamentous thermophilic fungus commonly isolated from hot
environments such as geothermal springs and mine shafts. They belong to the genus
Geosmithia which includes species that were formerly classified as Penicillum. Due to its
morphological similarities to Penicillium, G. argillacea has often previously been
overlooked and misidentified as other pathogens such as members of the Paecilomyces
genus. The fungus has rough septate conidiophores bearing monoverticillate, biverticillate,
or terverticillate penicilli. It has cylindrical phialides with tapered tips, producing smooth­walled cylindrical or box-shaped conidia measuring 3.0–4.5 by 1.5–2.7 μm, which later
appear ovoid upon maturity.

G. argillacea has been repeatedly isolated from sputum samples of patients with cystic
fibrosis (CF). They form chronic colonies in the airways of CF patients with potential to
cause invasive mycosis and disseminated or systemic disease in patients that have
become immunocompromised. It has also been revealed that all fungal strains isolated
from patients with chronic granulomatous disease (CGD) that were previously identified as
Paecilomyces variotii were in fact G. argillacea. G. argillacea shows a high level of
resistance to standard antimycotics leading to the requirement of surgical intervention in
some cases and a high fatality rate.

Introduction to Geosmithia argillacea

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MIN

MAX

The PCR Max qPCR Kit for Geosmithia argillacea (G.argillacea) genomes is designed for
the in vitro quantification of G.argillacea genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the G.argillacea genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
G.argillacea sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• G.argillacea specific primer/probe mix (150 reactions BROWN)

FAM labelled

• G.argillacea positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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