PCRmax Fusarium (all species) Instruction Manual

Translation elongation factor
alpha (EF-1a) gene

Fusarium_spp

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

1

The genus Fusarium collectively represents an important group of fungal plant pathogens,
causing various diseases on nearly every economically important plant species. Of equal
concern is the health hazard posed to humans and livestock by the plethora of Fusarium
mycotoxins. Besides their economic importance, species of Fusarium also serve as key
model organisms for biological and evolutionary research.

The genus includes a number of economically important plant pathogenic species.
Genome size varies amongst species; e.g. FV3 ~41.7 Mbp, FG3 ~36Mbp, FO ~60Mbp.
Our kit targets the Translation elongation factor alpha (EF-1a) gene across all Fusarium
species.

Members of the Fusarium oxysporum are the most common phytopathogenic Fusaria.
They cause wilts of over 100 cultivated plant species, including tomato, potato, sugarcane,
bean, cowpea, date and oil palm, as well as cooking and dessert bananas. The primary
solution to control such diseases is through the development of disease resistant plant
cultivars.

Plant disease resistance genes have been identified for the effective control of tomato wilt
but new races of the pathogen continue to develop overcoming deployed resistance and
thwarting tomato breeding efforts. Because Fusarium is a long-lived, soil-borne pathogen,
infested soil remains contaminated indefinitely, so only resistant varieties can be grown on
that site.

Fusarium graminearum commonly infects barley if there is rain late in the season. It is of
economic impact to the malting and brewing industries, as well as feed barley. Fusarium
contamination in barley can result in head blight, and in extreme contaminations, the barley
can appear pink. F. graminearum can also cause root rot and seedling blight.

F. verticillioides is a pathogen of maize and sorghum, and produces carcinogenic
mycotoxins known as fumonisins.

Some species may cause a range of opportunistic infections in humans. In humans with
normal immune systems, fusarial infections may occur in the nails and in the cornea. In
humans whose immune systems are weakened via neutropenia aggressive infections
penetrating the entire body and bloodstream may be caused by members of the Fusarium
solani complex, Fusarium oxysporum, Fusarium verticillioides, Fusarium proliferatum and,
rarely, other fusarial species.

Introduction to Fusarium_spp

2

MIN

MAX

The PCR Max qPCR Kit for Fusarium_spp (Fusarium_spp) genomes is designed for the in
vitro quantification of Fusarium_spp genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the Fusarium_spp genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
Fusarium_spp sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

3

Kit Contents

• Fusarium_spp specific primer/probe mix (150 reactions BROWN)

FAM labelled

• Fusarium_spp positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

4