PCRmax Fungi Kingdom (including yeast) Instruction Manual
Ribosomal RNA (18S) gene
Fungi & Amoebozoa
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Fungi, also known as Eumycota, are a diverse range of organisms encompassing yeasts,
moulds and mushrooms. They are eukaryotic organisms such that their cells contain
membrane bound organelles and they have DNA genomes that have both coding and noncoding sequence. They have a rigid cell wall, making them non-motile, which distinguishes
them from animals. The characteristic cell wall components are chitin and glucans,
primarily alpha-glucans. They digest food externally of the organism by releasing enzymes
into the surrounding environment. These enzymes break down organic matter into a form
that the fungus can absorb. Fungi inhabit almost every niche in the environment and
humans are exposed to these organisms in every field of life.
They are characterized by non-motile bodies constructed of apically elongating filaments
called the hyphae. Fungi reproduce sexually, asexually or parasexually. When two mating
types fuse, the two nuclei do not fuse necessarily fuse but can coexist until the right
conditions are present. Prior to mating in sexual reproduction, individual fungi
communicate with other individuals chemically via pheromones. Some of the fungi produce
bioactive compounds called mycotoxins such as alkaloids and polyketides that are toxic to
animals including humans. Several species of fungi are pathogenic which can lead to great
economic loss as they destroy vast acres of crops, cause food spoilage and animal and
human disease. In contrast, they are also widely used as a direct food source, in the
production of food, drink, protein, detergent and antibiotics, as well as being used as
pesticides.
Introduction to Fungi & Amoebozoa
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MIN
MAX
Our kit for fungi has been designed for the specific and exclusive in vitro quantification of
all fungi species including yeasts. The 18S ribosomal gene, is the ideal target to achieve
a broad based detection profile within this Kingdom. The primers and probe sequences
in this kit have 100% homology with a broad range of reference sequences based on a
comprehensive bioinformatics analysis.
The PCR Max qPCR Kit for Fungi & Amoebozoa (Fungi&Amoebozoa) genomes is
designed for the in vitro quantification of Fungi&Amoebozoa genomes. The kit is designed
to have the broadest detection profile possible whilst remaining specific to the
Fungi&Amoebozoa genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
Fungi&Amoebozoa sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• Fungi&Amoebozoa specific primer/probe mix (150 reactions BROWN)
FAM labelled
• Fungi&Amoebozoa positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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