PCRmax Francisella tularensis Instruction Manual

succinate dehydrogenase (sdhA)
gene

Francisella tularensis

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Francisella tularensis is an aerobic, Gram-negative, non-motile and non-sporing
Coccobacillus which is the causative agent of the disease Tularemia, an infectious disease
which is highly virulent in humans and rabbits. F. tularensis has a DNA genome of almost

1.9M nt in length arranged in a circular conformation and encoding under 2000 genes.
There are several subspecies of this bacterium which vary in their degree of virulence. The
highly virulent Francisella tularensis Type A strain is found mainly in North America
whereas the less virulent F. tularensis palaeartica Type B strain is found in Europe and
Eurasia. Two other non-virulent strains are less well studied and found in central Asia.

This bacterium uses arthropods as vectors to infect humans and animals but can also be
transmitted via the air. Infection occurs via the mucosal membranes of the lung and
gastrointestinal tract and also through the skin using its type 4 pili to adhere to the host
cells. From here it targets organs including the lymph nodes, liver, spleen and lungs.
Within the host, the bacterium infects macrophages by phagocytosis and multiplies until
the host cell bursts open, releasing bacteria to infect other cells.

Infection with this bacterium results in a debilitating disease even at low levels of infection.
Symptoms may vary depending on the mode of infection with the most common type of
infection via insect bite causing an ulcer at the bite site accompanied by fever, chills,
headache and fatigue. Infection via inhalation causes a typhoidal disease with fever,
fatigue, weight loss and pneumonia. There is no vaccine available against this bacterium
with most cases of infection being treated with antibiotics.

Introduction to Francisella tularensis

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MIN

MAX

Our kit for Francisella tularensis has been designed for the specific and exclusive in vitro
quantification of this species including the three sub species holarctica, mediasiatica and
tularensis . The primers and probe sequences in this kit have 100% homology with a
broad range of clinically relevant reference sequences based on a comprehensive
bioinformatics analysis.

The PCR Max qPCR Kit for Francisella tularensis (F.tularensis) genomes is designed for
the in vitro quantification of F.tularensis genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the F.tularensis genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
F.tularensis sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• F.tularensis specific primer/probe mix (150 reactions BROWN)

FAM labelled

• F.tularensis positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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