For general laboratory and research use only
Enterococcus faecalis is a Gram-positive commensally bacterium inhabiting the
gastrointestinal tracts of humans and other mammals. Like other species in the genus
Enterococcus, E. faecalis can cause life-threatening infections in humans, especially in the
nosocomial (hospital) environment: the naturally high levels of antibiotic resistance found in
E. faecalis contribute to its pathogenicity. E. faecalis presents as a non-motile
microorganism that is facultatively anaerobic, it ferments glucose without gas production,
and does not produce a catalase reaction with hydrogen peroxide. It produces a reduction
of litmus milk, but does not liquefy gelatin. Growth of nutrient broth is consistent with being
facultatively anaerobic.
E. faecalis can cause endocarditis, as well as bladder, prostate, and epididymal infections;
nervous system infections are less common.
E. faecalis is resistant to many commonly used antimicrobial agents (aminoglycosides,
aztreonam, cephalosporins, clindamycin, the semi-synthetic penicillins nafcillin and
oxacillin, and trimethoprim-sulfamethoxazole). Exposure to cephalosporins is a particularly
important risk factor for colonization and infection with enterococci.
Introduction to Enterococcus faecalis
The primers have 100% homology with all reference sequences included in the NCBI
database (AF335185.1 and AE016830.1) therefore have a very broad quantification
profile.
The PCR Max qPCR Kit for Enterococcus faecalis (E.faecalis) genomes is designed for
the in vitro quantification of E.faecalis genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the E.faecalis genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
E.faecalis sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Kit Contents
• E.faecalis specific primer/probe mix (150 reactions BROWN)
FAM labelled
• E.faecalis positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit is designed to work well with all processes that yield high quality DNA with
minimal PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes