PCRmax Enterococcus caseliflavus Instruction Manual
Superoxide dismutase A (SodA)
gene
Enterococcus
caseliflavus
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Enterococcus casseliflavus is a Gram-positive, motile, catalase-negative bacterium
(originally termed as Streptococcus faecium var casseliflavus). Its genome is a linear DNA
chromosome about 3.4Mnt long consisting of 3283 protein coding genes. This
opportunistic pathogen can cause urinary tract infections, bacteraemia and infective
endocarditis.
E. casseliflavus can normally be found in the gastrointestinal tract but is capable of causing
infection by colonisation especially after hospitalisation. Transmission can be via direct
contact with contaminated faeces, blood or urine or via indirect contact. The pathogenesis
of E. casseliflavus is not well established but it is thought that the plasmid encoded
proteins haemolysin or bacteriocin may be virulence factors causing pathogenesis by
lysing erythrocytes.
This bacterium has intrinsic resistance to vancomycin and acquired resistance to several
other antibiotics and so if the strain is susceptible, ampicillin and penicillins are the drugs of
choice. Efforts to prevent direct contact with infected individuals are standard practice in
hospitals.
Introduction to Enterococcus caseliflavus
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MIN
MAX
Our kit for E. casseliflavus has been designed for the specific and exclusive in vitro
quantification of all E. casseliflavus strains. Super oxide dismutase has previously been
shown to be a good target sequence for the differential detection of enterococcus species
in other clinical real time PCR based studies (B.A. Layton et.al., 2010). The primers and
probe sequences in this kit have 100% homology with a broad range of clinically relevant
reference sequences based on a comprehensive bioinformatics analysis.
The PCR Max qPCR Kit for Enterococcus caseliflavus (E.caseliflavus) genomes is
designed for the in vitro quantification of E.caseliflavus genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the E.caseliflavus
genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
E.caseliflavus sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• E.caseliflavus specific primer/probe mix (150 reactions BROWN)
FAM labelled
• E.caseliflavus positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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