PCRmax Encephalitozoon (all species) Instruction Manual

16S ribosomal RNA (18S) gene

Encephalitozoon

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Encephalitozoon is a genus of fungi of the microsporidium phylum containing species with
very small genomes of just over 2.4Mbp in length. Species within this genus form spores of
1-3um in diameter containing a posterior vacuole, anterior anchoring disc and polaroplast.
Microsporidia species infect many animals including humans and can cause
microsporidiosis and keratoconjunctivitis, most commonly in immunocompromised
individuals.
Spores of the encephalitozoon species are hardy and can survive in the environment for
long periods of time. Spores are inhaled or ingested and once within the host, extends a
polar tubule which is used to inject the sporoplasm into the host cell. The sporoplasm
replicates by merogony or sporogony and does so in parasitophorous vacules within the
cytoplasm. Meronts are formed around the edge of the vacuole and differentiate into
sporoblast and spores as they migrate to the center of the vacuole. As the spores multiply
and fill the host cell, the cell membrane is disrupted, releasing the spores to infect other
cells and shed in excrement.
There are four species which commonly infect humans with some resulting clinical
symptoms including diarrhea and cholecystits (E. intestinalis) while others causes
encephalitis (E. cuniculi) and Keratoconjunctivitis, an inflammation of the cornea and
conjunctiva (E. hellum). Treatment with antimicrobial agents such as albendazole and
fumagillin usually clears the infection.

Introduction to Encephalitozoon

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MIN

MAX

Our kit for Encephalitozoon has been designed for the specific and exclusive in vitro
quantification of this genus . The 16S ribosomal gene, is the ideal target to achieve a
broad based detection profile for all species within this genus. The primers and probe
sequences in this kit have 100% homology with a broad range of clinically relevant
reference sequences based on a comprehensive bioinformatics analysis. Other closely
related species within the Unikaryonidae family are not detected.

The PCR Max qPCR Kit for Encephalitozoon (Encephalitozoon) genomes is designed for
the in vitro quantification of Encephalitozoon genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the Encephalitozoon
genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
Encephalitozoon sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• Encephalitozoon specific primer/probe mix (150 reactions BROWN)

FAM labelled

• Encephalitozoon positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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