PCRmax Eco 48 Real-Time qPCR System Instruction Manual
Eco Study Software
Operation Manual
PCRmax rev A.1
Intended use
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Contents
Page
Intended use 2
Contents 3
Introduction 5
What is Eco 48 Study? 5
Requirements for a standard curve study 5
Requirements for a Relative Quantification Study 5
Adding an Experiment 6
Designating the Mother Plate 6
Designating Plate Control Wells in your Study 7
Specifying Analysis Settings for your Study 8
Analyzing a Standard Curve Study 8
Analyzing a Relative Quantification Study 8
Reviewing the Data in your Study 10
Well table 10
Amplification plot 11
Melt Curve 12
Results Tab 12
Standard Curve 12
Relative Quantification 13
Exporting Study Data to a Report or Presentation 13
Technical Assistance 14
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PCRmax rev A.1
Introduction
What is Eco 48 Study?
The Eco 48 Study software enables you to combine data from multiple Eco 48 experiments into a study for analysis.
Currently, only Standard Curve and Relative Quantification experiments are supported.
NOTE Cole-Parmer Ltd does not recommend combining experiments from multiple instruments because
differences in flourescence values between instruments might increase the systematic error in your
measurements.
Requirements for a Standard Curve Study
To ensure successful analysis, a Standard Curve study must contain:
• One or more experiments of the same type, with the same thermal profile (except for melt profile) and
number of thermal cycles
• One mother plate (if the study includes more than one experiment)
• At least two standards with different values
• One or more plate control wells in each plate
Requirements for a Relative Quantification Study
To ensure successful analysis, a Relative Quantification study must contain:
• One or more experiments of the same type, with the same thermal profile (except for melt profile) and
number of thermal cycles
• One mother plate (if the study includes more than one experiment)
• One or more plate control wells in each plate
• One reference sample against which the target (non-reference) samples will be analyzed
• At least one target (non-reference) assay
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