PCRmax Cryptococcus neoformans Instruction Manual

Hypothetical protein

Cryptococcus
neoformans

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Cryptococcus neoformans is a yeast-like fungi of the Sporidiobolaceae family and is a
known cause of Cyrptococcosis, a disease mainly affecting immune-compromised
individuals. The fungus measures between 4 and 6µm in diameter and is surrounded by a
polysaccharide capsule.

The fungi enter the lungs by inhalation from the environment where it can often be found in
soil. A pulmonary form of the disease can occur at the primary site of infection. However,
dissemination of the fungi from the lungs can lead to infections of the central nervous
system causing meningitis or other areas of the body causing diseases including skin
lesions, conjunctivitis, myocarditis, renal abscesses and arthritis.

Acute pulmonary infection is usually asymptomatic except in immune-suppressed
individuals, especially those with AIDS. Chronic pulmonary infections can lead to nodule
formation, segmental pneumonia and lymphadenopathy. These infections can usually be
treated with a course of anti-fungal medicines.

This species has 3 variants that can be found in geographically varied locations. Variant
grubii and neoformans can be found globally whilst variant gattii is found mostly in the
tropics but has also off the south-eastern coast of Canada.

Introduction to Cryptococcus neoformans

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MIN

MAX

The PCR Max qPCR Kit for Cryptococcus neoformans (C.neoformans) genomes is
designed for the in vitro quantification of C.neoformans genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the C.neoformans
genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
C.neoformans sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• C.neoformans specific primer/probe mix (150 reactions BROWN)

FAM labelled

• C.neoformans positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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