PCRmax Clostridium sporogenes Instruction Manual

16S ribosomal RNA gene

Clostridium
sporogenes

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Clostridium sporogenes is the name given to strains of Clostridium botulinum that do not
produce botulinum neurotoxins (despite being closely related to group 1 C. botulinum
which are toxic) . In essence, they are considered virtually identical except for the
presence of the genes for botulinum toxin.

Being non-toxic they can cause food spoilage but pose no risk to human health. For this
reason it is important to be able to distinguish between C. sporogenes and other
Clostridium which may be toxicogenic.

Like other strains of C. botulinum, it is an anaerobic, motile, Gram-positive, rod-shaped
bacterium that produces oval, subterminal endospores and is commonly found in soil

Similarly, like other clostridium groups I & II, C. sporogenes has a genome of around

4.1Mbp arranged as a single, circular chromosome with a plasmid.
Genomically C. sporogenes is 4.1 million bp long with approx. 4,053 protein-coding genes
and 45 structural RNAs.

Introduction to Clostridium sporogenes

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MIN

MAX

Due to the fact that Clostridium sporogenes are simply strains of Clostridium botulinum
that do not produce the botulinum neurotoxins, this kit will also detect C.botulinum.

The PCR Max qPCR Kit for Clostridium sporogenes (C.sporogenes) genomes is designed
for the in vitro quantification of C.sporogenes genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the C.sporogenes genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
C.sporogenes sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• C.sporogenes specific primer/probe mix (150 reactions BROWN)

FAM labelled

• C.sporogenes positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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