PCRmax Clostridium estertheticum Instruction Manual
triosephosphate isomerase (tpi)
gene
Clostridium
estertheticum
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Cl. estertheticum causes spoilage of (raw) chilled vacuum-packed red meat, including
beef, lamb and venison and renders it completely unacceptable, resulting in substantial
financial losses and reduced consumer confidence.
A number of other species of Clostridium, including Cl. algidicarnis, Cl. putrefaciens, and
Cl. gasigenes are also capable of multiplying at low temperature, and have also been
implicated in this type of red meat spoilage. However, Cl. estertheticum is the most
common. Cl. estertheticum is psychrophilic and capable of growing relatively rapidly in the
chilled, anaerobic conditions used to store vacuum packed meat at temperatures down to
minus 1.5°C. The bacteria metabolise compounds such as glucose and lactic acid, leading
to production of various foul-smelling compounds, and also carbon dioxide and hydrogen thus ‘blown pack’ spoilage.
Meat can become contaminated from the live animals or indirectly (via contaminated
surfaces and equipment) from vacuum-packed meat that has been opened and
reprocessed in the same environment.
Our kit specifically targets the Cl. estertheticum triosephosphate isomerase (tpi) gene.
Introduction to Clostridium estertheticum
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MIN
MAX
The PCR Max qPCR Kit for Clostridium estertheticum (C.estertheticum) genomes is
designed for the in vitro quantification of C.estertheticum genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the C.
estertheticum genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
C.estertheticum sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• C.estertheticum specific primer/probe mix (150 reactions BROWN)
FAM labelled
• C.estertheticum positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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