PCRmax Chlamydophila abortus Instruction Manual

Outer membrane protein A
(ompA) gene

Chlamydophila abortus

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Chlamydophila abortus is an obligate Gram-negative bacterium of the Chlamydiaceae
family with two membranes and no flagella. The genome of this species is circular in
conformation with a total length of 1.14 Mbp and contains 961 genes, not all of which have
a known function. C. abortus infection can be found in livestock such as cows, goats, pigs
and sheep causing abortion and therefore presenting a significant zoonotic risk.

The metabolically inactive, infectious form (elementary body) bind to host oviduct cells
triggering phagocytosis. This results in the bacterium residing in a protective vacuole which
enables differentiation of the elementary body to a reticulate body and evasion of the host
immune system. Within this inclusion vacuole, binary fission occurs over several days and
is followed by transformation back to elementary bodies which are subsequently release by
cell lysis or exocytosis enabling infection of neighbouring cells.

In females livestock infection can cause abortion which may be associated with retained
placenta and uterine discharge or sterility. In males, C. abortus infections may lead to
formation of atrophic testes. Other symptoms can also include cough, keratoconjunctivitis,
arthritis and encephalitis.

Introduction to Chlamydophila abortus

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MIN

MAX

Our kit for C. abortus has been designed for the specific and exclusive in vitro
quantification of this species and does not detect other Chlamydophila. A highly
conserved sequence within the ompA gene has previously been shown to be a good
target sequence in other clinical real time PCR based studies (Pantchev A et.al.,2009).
The primers and probe sequences in this kit have 100% homology with a broad range of
clinically relevant reference sequences based on a comprehensive bioinformatics
analysis.

The PCR Max qPCR Kit for Chlamydophila abortus (C.abortus) genomes is designed for
the in vitro quantification of C.abortus genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the C.abortus genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
C.abortus sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• C.abortus specific primer/probe mix (150 reactions BROWN)

FAM labelled

• C.abortus positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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