PCRmax Candidatus Mycoplasma turicensis Instruction Manual

16S ribosomal RNA

Candidatus
Mycoplasma turicensis

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Candidatus Mycoplasma turicensis (Mtc) is one of three Haemoplasmas (Candidatus
Mycoplasma haemominutum, Candidatus Mycoplasma turicensis and Mycoplasma
haemofelis) that until recently were collectively classified as Haemobartonella felis. PCR
analysis of 16s rRNA sequences revealed evidence for Candidatus Mycoplasma turicensis
as a separate haemotropic mycoplasma found in domestic cats.

Candidatus M. turicensis has been identified in cats as a cause of Feline Infectious
Anaemia (FIA), but appears to be less pathogenic than Mycoplasma haemofelis. The
mode of transmission for this haemoplasma is not fully understood however blood sucking
arthropod vectors, fleas, mosquitos and ticks are thought to be a primary mode. Biting and
scratching is also a possible route of infection and transmission has been observed to
occur vertically from mother to offspring. Once the parasite enters the blood stream, it
attaches to host red blood cells with polar filaments before invading the cells and triggering
the cat's immune response, releasing antibodies. The antibodies bind to the foreign Mtc
proteins as a coating, which serves to mark the infected red blood cell for removal and
destruction. Due to the large numbers of infected red blood cells being destroyed, the cat
becomes anaemic and pale.

Haemoplasmas are the causative agent for a range of feline diseases such as feline
conjunctivitis, lower respiratory tract infections, feline infectious peritonitis and feline
infectious anaemia (FIA). Clinical signs of the infection include anorexia, depression,
lethargy, weakness, fever and weight loss. Cats that have been infected with a
Haemoplasma may remain permanent carriers and infection can re-emerge through stress.

Introduction to Candidatus Mycoplasma turicensis

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MIN

MAX

The PCR Max qPCR Kit for Candidatus Mycoplasma turicensis (C.M.turicensis) genomes
is designed for the in vitro quantification of C.M.turicensis genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the C.M.turicensis
genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
C.M.turicensis sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• C.M.turicensis specific primer/probe mix (150 reactions BROWN)

FAM labelled

• C.M.turicensis positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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