PCRmax Candidatus Mycoplasma haemominutum Instruction Manual

gapA gene

Candidatus
Mycoplasma
haemominutum

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Candidatus Mycoplasma haemominutum (Mhm) is one of three Haemoplasmas
(Candidatus Mycoplasma haemominutum, Candidatus Mycoplasma turicensis and
Mycoplasma haemofelis) that until recently were collectively classified as Haemobartonella
felis. There is evidence to suggest that the Ohio variant and California variant of H. felis
are in fact two distinct species, M. haemofelis and C. M. haemominutum respectively.

Candidatus M. haemominutum has been identified in cats as a cause of Feline Infectious
Anaemia (FIA), but appears to be less pathogenic than Mycoplasma haemofelis. The
mode of transmission for this haemoplasma is not fully understood however blood sucking
arthropod vectors, fleas, mosquitos and ticks are thought to be a primary mode. Biting and
scratching is also a possible route of infection and transmission has been observed to
occur vertically from mother to offspring. Once the parasite enters the blood stream, it
attaches to host red blood cells with polar filaments before invading the cells and triggering
the cat's immune response, releasing antibodies. The antibodies bind to the foreign Mhm
proteins as a coating, which serves to mark the infected red blood cell for removal and
destruction. Due to the large numbers of infected red blood cells being destroyed, the cat
becomes anaemic and pale.

Haemoplasmas are the causative agent for a range of feline diseases such as feline
conjunctivitis, lower respiratory tract infections, feline infectious peritonitis and feline
infectious anaemia (FIA). Clinical signs of the infection include anorexia, depression,
lethargy, weakness, fever and weight loss. Cats that have been infected with a
Haemoplasma may remain permanent carriers and infection can re-emerge through stress.

Introduction to Candidatus Mycoplasma
haemominutum

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MIN

MAX

The PCR Max qPCR Kit for Candidatus Mycoplasma haemominutum (C.M.haem)
genomes is designed for the in vitro quantification of C.M.haem genomes. The kit is
designed to have the broadest detection profile possible whilst remaining specific to the C.
M.haem genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
C.M.haem sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• C.M.haem specific primer/probe mix (150 reactions BROWN)

FAM labelled

• C.M.haem positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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