PCRmax Campylobacter jejuni Instruction Manual

CadF gene

Campylobacter jejuni

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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The genus Campylobacter are Gram-negative, spiral, microaerophilic bacteria. Motile, with
either uni- or bi-polar flagella, the organisms have a somewhat curved, rod-like
appearance, and are oxidase-positive. Campylobacter jejuni is now recognised as one of
the main causes of bacterial foodborne disease in many developed countries. At least a
dozen species of Campylobacter have been implicated in human disease, with C. jejuni
and C. coli the most common.

Campylobacteriosis is an infection by campylobacter. The common routes of transmission
are fecal-oral, person-to-person sexual contact, ingestion of contaminated food or water. It
produces an inflammatory, sometimes bloody, diarrhea, periodontitis or dysentery
syndrome, mostly including cramps, fever and pain. The infection is usually self-limiting
and in most cases, symptomatic treatment by reposition of liquid and electrolyte
replacement is enough in human infections. The use of antibiotics, on the other hand, is
controversial.

This is most commonly caused by C. jejuni, a spiral and comma shaped bacterium
normally found in cattle, swine, and birds, where it is non-pathogenic. But the illness can
also be caused by C. coli (also found in cattle, swine, and birds)

One cause of the effects of campylobacteriosis is tissue injury in the gut. The sites of
tissue injury include the jejunum, the ileum, and the colon. C jejuni appears to achieve this
by invading and destroying epithelial cells.

Introduction to Campylobacter jejuni

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MIN

MAX

The primers have 100% homology with all reference sequences included in the NCBI
database and therefore have a very broad detection profile for these two strains.

Accession number of detect C.jejuni strains
CP000814.1, AL111168.1, CP000025.1, AF104303.1, CP000538.1, CP000768.1,
AF104302.1,

The PCR Max qPCR Kit for Campylobacter jejuni (C.jejuni) genomes is designed for the in
vitro quantification of C.jejuni genomes. The kit is designed to have the broadest detection
profile possible whilst remaining specific to the C.jejuni genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
C.jejuni sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• C.jejuni specific primer/probe mix (150 reactions BROWN)

FAM labelled

• C.jejuni positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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