PCRmax Campylobacter fetus subspecies venerialis Instruction Manual

insertion sequence ISCfe1
C.fetus-venerialis
PCRmax Ltd qPCR test
TM
For general laboratory and research use only
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Campylobacter foetus is a rod shaped, motile, Gram-negative bacteria with a characteristic spiral curve morphology. Its genome has a circular DNA structure and codes for some 1820 genes. The bacterial pathogen is pathologically important in cattle and sheep where it can cause abortion. There are two distinct subspecies of this bacterium that cause infection by different means, the subspecies are Venerealis and Fetus.
Campylobacter venerialis is adapted specifically to the bovine genital tract where it is the causative agent of bovine genital campylobacteriosis. This is a venereal disease that is characterized by early embryonic death, infertility, an extended calving season and abortion. The bacterium resides in the mucus layer of the male pupuce and is sexually transmitted by natural mating or by artificial insemination.
Introduction to C.fetus-venerialis
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MIN
MAX
The target sequences selected are based on the work of C, Abril et,al., 2007 and are therefore highly specific. The primers have 100% homology with the three principal reference sequences within the NCBI database (AM260752.1, AM286431.1, AM286430.1).
The PCR Max qPCR Kit for C.fetus-venerialis (C.fetus-venerialis) genomes is designed for the in vitro quantification of C.fetus-venerialis genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the C.fetus-venerialis genome.
The primers and probe sequences in this kit have 100% homology with a broad range of C.fetus-venerialis sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will answer your question.
Specificity
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Kit Contents
• C.fetus-venerialis specific primer/probe mix (150 reactions BROWN)
FAM labelled
• C.fetus-venerialis positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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