PCRmax Campylobacter fetus Instruction Manual
putative fibronectin/fibrinogenbinding protein
Campylobacter fetus
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Campylobacter foetus is a rod shaped, motile, Gram-negative bacteria with a characteristic
spiral curve morphology. Its genome has a circular DNA structure and codes for some
1820 genes. The bacterial pathogen is pathologically important in cattle and sheep where it
can cause abortion. There are two distinct subspecies of this bacterium that cause
infection by different means, the subspecies are Venerealis and Fetus.
Campylobacter fetus interacts and disrupts reproduction cycles. It does this by either
causing infertility in the female cattle by infection transmitted from the mucus layer of the
male prepuce as in the case of the subspecies Venerealis. The subspecies Fetus infects
the host when ingested – this subspecies can also infect humans by this method resulting
in symptoms from severe intestinal inflammation to septicemia in immunocompromised
individuals.
Introduction to Campylobacter fetus
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MIN
MAX
The primers have 100% homology with the principal reference sequence within the NCBI
data base (CP000487).
The PCR Max qPCR Kit for Campylobacter fetus (C.fetus) genomes is designed for the in
vitro quantification of C.fetus genomes. The kit is designed to have the broadest detection
profile possible whilst remaining specific to the C.fetus genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
C.fetus sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• C.fetus specific primer/probe mix (150 reactions BROWN)
FAM labelled
• C.fetus positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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