PCRmax Burkholderia cepacia complex (all species) Instruction Manual
RecA (recA) gene
Burkholderia cepacia
complex
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
1
The Burkholderia cepacia complex (Bcc) is a collection of nine genotypically distinct but
phenotypically similar species within the genus Burkholderia. They are Gram- negative,
non-spore forming β-proteobacteria and exhibit an extraordinary metabolic versatility,
allowing them to adapt to a wide range of environments. The genomes of Bcc bacteria
range from 6.2 to 8.7 Mbp, and are organized as 3 circular chromosomal replicons and one
to five megaplasmids.
Several strains of Bcc bacteria have the ability to degrade pollutants of water and soil like
crude oil, herbicides and xenobiotics. Some strains are also capable of producing
antifungal compounds and fixing atmospheric nitrogen. However these bacteria are still
considered as important human pathogens because of their ability to develop chronic
infections in immunocompromised patients.
In patients with cystic fibrosis, Bcc strains can colonise the major airways leading to
aggravation of pulmonary infection. The outcome of Bcc infections in patients with cystic
fibrosis can vary from asymptomatic carriage, cepacia syndrome, that is characterised by
recurrent episodes of fever and bacteraemia, or even death. Bcc bacteria can also cause
lung infections in chronic granulomatous patients leading to pneumonia and septicaemia
and are often lethal. In additon, they are seen to cause bacteraemia in patients with
diabetes mellitus, pharyngeal infections, congestive heart failure and malignancy.
Bcc infections can be spread patient to patient, both within and outside the hospital and
often the infected patients are kept isolated to limit the spread. The treatment usually
involves use of multiple antibiotics including doxycycline, piperacillin and chloramphenicol
Introduction to Burkholderia cepacia complex
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MIN
MAX
Burkholderia cepacia complex (BCC), is a group of catalase-producing, non-lactosefermenting Gram-negative bacteria composed of at least seventeen different species,
including B. cepacia, B. multivorans, B. cenocepacia, B. vietnamiensis, B. stabilis, B.
ambifaria, B. dolosa, B. anthina, and B. pyrrocinia
The PCR Max qPCR Kit for Burkholderia cepacia complex (B.cepacia_complex) genomes
is designed for the in vitro quantification of B.cepacia_complex genomes. The kit is
designed to have the broadest detection profile possible whilst remaining specific to the B.
cepacia_complex genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
B.cepacia_complex sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• B.cepacia_complex specific primer/probe mix (150 reactions BROWN)
FAM labelled
• B.cepacia_complex positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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