PCRmax Brucella genus (all species) Instruction Manual
L-glutamine: 2-deoxy-scylloinosose amino-transferase
Brucella genus (all
species)
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
1
The Brucella genus is a genus of Gram-negative, non-motile bacteria that are rod shaped.
Species within this genus cause Brucellosis in many species of mammals. Brucella
species have a genome of around 3.2M nucleotides in length split over two circular
chromosomes.
All Brucella species cause infections in specific animal groups that lead to similar diseases.
Differences between the bacterium within this genus can be found in genes coding for
surface exposed proteins. B. suis infects swine, whereas B. melitensis infects goats and
camels B. canis infects dogs. Cattle are mainly infected by B. abortis, with B. ovis infecting
sheep and B. pinnipediae recently though to infect marine mammals. These bacterial
species cause male infertility resulting from orchitis and epididymitis and may also cause
abortion in females due to andometritis and placentitis.
These bacteria are also capable of infecting humans and can be transmitted via inhalation
of infected animal waste products, direct contact with open wounds or ingestion of
contaminated food subatnaces such as un-pasteurised milk. In humans, Brucella infection
can result in headache, fatigue, fever, sweating, weight loss and muscle pain. The human
infection can be treated by a course of antibiotics in combination to prevent symptoms.
Introduction to Brucella genus (all species)
2
MIN
MAX
The primers have 100% homology with all Brucella reference sequences included in the
NCBI database and therefore have a very broad detection profile (CP001488.1,
AE008917.1, P000887.1, CP000911.1, CP000872.1, CP000708.1,
AE014291.4AF047478.1, AM040264.1, AE017223.1).
The PCR Max qPCR Kit for Brucella genus (all species) (Brucella_spp) genomes is
designed for the in vitro quantification of Brucella_spp genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the Brucella_spp
genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
Brucella_spp sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
3
Kit Contents
• Brucella_spp specific primer/probe mix (150 reactions BROWN)
FAM labelled
• Brucella_spp positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
4
Loading...