PCRmax Blastocystis genus (all species) Instruction Manual
G elongation factor-1 alpha gene
Blastocystis species
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Blastocystis is a common microscopic organism that inhabits the intestine and is found
throughout the world. A full understanding of the biology of Blastocystis and its relationship
to other organisms is not clear. Infection with Blastocystis is called blastocystosis.
Blastocystis spp have around 18.82 mBp and over 6000 coding genes. Our kit targets the
G elongation factor-1 alpha gene.
How Blastocystis is transmitted is not known for certain, although the number of people
infected seems to increase in areas where sanitation and personal hygiene is not
adequate. Studies have suggested that risk of infection may increase through: ingesting
contaminated food or water, exposure to a day care environment, or exposure to animals.
Symptoms of blastocystis include: Watery or loose stools, diarrhea, abdominal pain, anal
itching, weight loss, constipation, and excess gas have all been reported in persons with
Blastocystis infection. Many people have no symptoms at all. The organism can be found
in both well and sick persons.
Introduction to Blastocystis species
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MIN
MAX
The PCR Max qPCR Kit for Blastocystis species (Blastocystis_spp) genomes is designed
for the in vitro quantification of Blastocystis_spp genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the Blastocystis_spp
genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
Blastocystis_spp sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• Blastocystis_spp specific primer/probe mix (150 reactions BROWN)
FAM labelled
• Blastocystis_spp positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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