PCRmax Aureobasidium pullulans Instruction Manual
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Aureobasidium
pullulans
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Aureobasidium pullulans is a yeast-like fungus of the family Dothioraceae. It is a common
outdoor mold, found on a wide range of plant species as an epiphyte or endophyte. The
spores have no negative effect on the plant and do not cause any symptoms of disease.
Indoors, A. pullulans prefers wet surfaces with water such as shower curtains, tile grout
and caulking. It will grow on wood, leather and cloth, and also water-based paints. It can
grow on certain foods as well, such as grains and fruits. Clinically, A. pullulans is
recognized as a serious allergen.
While in the mildew form, A. pullulans can only become airborne if the water it is in
becomes aerosolized. However, it can produce airborne allergens once it dries out, and
has been found in high concentrations in home environments. A study conducted found
over 60% of patients with an allergy and/or asthma produced a positive skin test response
to A. pullulans. Sensitivity to A. pullulans is significantly associated with more severe
asthma.
Introduction to Aureobasidium pullulans
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MIN
MAX
The PCR Max qPCR Kit for Aureobasidium pullulans (A.pullulans) genomes is designed
for the in vitro quantification of A.pullulans genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the A.pullulans genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
A.pullulans sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• A.pullulans specific primer/probe mix (150 reactions BROWN)
FAM labelled
• A.pullulans positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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