PCRmax Aspergillus Instruction Manual

18S ribosomal RNA gene,
transcribed spacer 1, 5.8S
ribosomal RNA gene

Aspergillus all
pathogenic subtypes

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Aspergillosis is the name given to a wide variety of diseases caused by the genus of fungi
Aspergillus. The most common forms are allergic bronchopulmonary aspergillosis,
pulmonary aspergilloma and invasive aspergillosis. Compromised immune systems often
allow Aspergillus to colonize.
A. fumigatus has a stable haploid genome, with no known sexual cycle, and reproduces by
forming conidiospores that are released into the environment. Capable of growth at 37°C
(human body temperature), spores are common inhalation pollutants; typically, however,
these are quickly eliminated by the immune system in healthy individuals.
A flavus is a mold fungus associated with aspergillosis of the lungs and sometimes
believed to cause corneal, otomycotic, and nasoorbital infections. It is believed to be
allergenic. It is particularly common on corn and peanuts, as well as water damaged
carpets, and is one of several species of mold known to produce aflatoxin which is a
carcinogenic substance.
A. clavatus is a species of Aspergillus with conidia dimensions 3-4.5 x 2.5-4.5
micrometres. It is found in soil and animal manure and can produce the toxin patulin which
may be associated with disease in humans and animals. This species is only occasionally
pathogenic but is link to human allergic disease.

Introduction to Aspergillus all pathogenic subtypes

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MIN

MAX

The ribosomal gene, is the ideal target to achieve a broad based detection profile within
this genus. The primers and probe sequences in this kit have 100% homology with a
broad range of clinically relevant reference sequences based on a comprehensive
bioinformatics analysis.

In vitro testing has also found that this kit will detect certain strains of Candida albicans.

The PCR Max qPCR Kit for Aspergillus all pathogenic subtypes (Aspergillus_spp)
genomes is designed for the in vitro quantification of Aspergillus_spp genomes. The kit is
designed to have the broadest detection profile possible whilst remaining specific to the
Aspergillus_spp genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
Aspergillus_spp sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• Aspergillus_spp specific primer/probe mix (150 reactions BROWN)

FAM labelled

• Aspergillus_spp positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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