PCRmax Ancylostoma duodenale Instruction Manual

5.8S ribosomal RNA

Ancylostoma
duodenale

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

1

Ancylostoma duodenale is a species of nematode worm of the Ancylostomatidae family.
This species is known to infect humans predominately in North Africa, the Middle East and
India causing Ankylostomiasis, a progressive anaemia. This species, more commonly
known as hookworm, are approximately 10mm in length with the females being longer than
the males. The anterior end accommodates a buccal capsule with two pairs of teeth used
for attaching to the host cells.

In ideal conditions, moist soil above 18°C, the eggs of this species can hatch revealing an
infective larva which can remain viable in the soil for several weeks. These larvae
penetrate the skin of humans, mainly on the foot when walking through contaminated soil.
Once inside the body, they migrate through the circulatory system to the lungs, up the
trachea and down the oesophagus through the stomach ending in the small intestine. The
larvae may mature into adults here and cause an infection over several months or may
become dormant resulting in a prolonged infection. In the intestine the adults mate and
new eggs are passed in the faeces.

An allergic reaction at the entry site is often seen in infected individuals as is an
inflammation of the lungs as the larvae penetrate the alveoli. Diarrhoea and stomach pains
are the result of the hookworm in the intestine. High parasitic levels can result in anaemia
due to blood loss and in children this can result in growth retardation.

Before the larvae migrate away from the skin, it can be treated with cryotherapy. Once it is
moving through the body Benzimidazole based therapies can be used to target the
infection and act by preventing nicrotuble polymerisation within the parasite. If anaemia
occurs then patients are also treated with furrous sulphate.

Introduction to Ancylostoma duodenale

2

MIN

MAX

Our kit for ancylostoma has been designed for the specific and exclusive in vitro
quantification of species within this genus and excluding members of the uncinaria
genus . The primers and probe sequences in this kit have 100% homology with a broad
range of clinically relevant reference sequences based on a comprehensive
bioinformatics analysis.

The PCR Max qPCR Kit for Ancylostoma duodenale (A.duodenale) genomes is designed
for the in vitro quantification of A.duodenale genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the A.duodenale genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
A.duodenale sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

3

Kit Contents

• A.duodenale specific primer/probe mix (150 reactions BROWN)

FAM labelled

• A.duodenale positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

4