PCRmax Anaplasma centrale Instruction Manual
major surface protein 2 (msp2)
gene
Anaplasma centrale
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
1
Anaplasma centrale is a Gram-negative bacterium of the Rickettsiales order lacking a
traditional cell wall. The double-stranded, circular genome of this species is around 1.2 Mb
in length, coding for over 900 proteins. Infection causes a mild form of anaplasmosis in
cattle and has been used as a vaccine against A. marginale. It occurs primarily in warm
tropical and subtropical areas.
A. centrale is most commonly transmitted by ticks such as Boophilus spp. and
Dermacentor spp but can also be transmitted via contaminated needles, dehorning
equipment, castrating knives, tattoo instruments, biting flies and mosquitoes.
It multiplies within the tick vectors before being passed to cattle when the ticks feed. The
bacteria infect the erythrocytes, thrombocytes and leukocytes of the cattle and mainly
multiply within the cells of the spleen and liver. The host immune system destroys infected
cells leading to anemia.
Cattle infection presents with fever, loss of appetite, jaundice, anemia and respiratory
problems. The infected animal may recover from the clinical symptoms of the infection but
will remain a carrier of the bacteria, passing it back to ticks during feeding. Immunity can
be passed to calves via milk feeding and offers protection to infants. In severe case, the
infection may result in animal fatality.
Introduction to Anaplasma centrale
2
MIN
MAX
The PCR Max qPCR Kit for Anaplasma centrale (A.centrale) genomes is designed for the
in vitro quantification of A.centrale genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the A.centrale genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
A.centrale sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
3
Kit Contents
• A.centrale specific primer/probe mix (150 reactions BROWN)
FAM labelled
• A.centrale positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
4
Loading...