PCRmax Ajellomyces capsulatus Instruction Manual

Internal transcribed spacer region
of the (ITS1) rRNA

Ajellomyces
capsulatus

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Ajellomyces capsulatus is a fungus of the Ajellomycetaceae family. This species has a
DNA genome of over 30M nucleotides and is a known cause of Histoplasmosis, a disease
of the lungs.

This dimorphic fungus changes through distinct stages controlled by environmental
temperature. In temperature around 25°C, such as in soil where the fungus is commonly
found, it is in a filamentous mycelia state. In the soil, the fungus produces spore which can
be inhaled as aerosols. In the lungs at 37°C it changes into budding yeast of up to 2µm in
length inside the host cell after phagocytosis.

Histoplamosis can infect anybody but especially the very young and very old or immuno­supressed individuals. This infection may be asymptomatic or can cause flu like symptoms
including fever and chest pains after an incubation period of around 10 days. The infection
can be treated with anti-fungal medicines and can be fatal if left untreated.

Introduction to Ajellomyces capsulatus

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MIN

MAX

The target sequence within the internal transcribed spacer region of the rRNA gene
complex has previously been shown to be a good genetic marker for A. capsulatus in
other clinical real-time PCR based studies (Jose Martagon-Villamil, et.al 2003). The
primers and probe sequences in this kit have 100% homology with a broad range of
clinically relevant reference sequences based on a comprehensive bioinformatics
analysis.

The PCR Max qPCR Kit for Ajellomyces capsulatus (A.capsulatus ) genomes is designed
for the in vitro quantification of A.capsulatus genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the A.capsulatus genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
A.capsulatus sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• A.capsulatus specific primer/probe mix (150 reactions BROWN)

FAM labelled

• A.capsulatus positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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