PCRmax Acinetobacter baumannii Instruction Manual

hypothetical protein sequence
gene

Acinetobacter
baumannii

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Acinetobacter baumannii is a Gram-negative bacterium of the Moraxellaceae family. The
genome of this bacterium is around 4Mbp in length, arranged in a single, circular
chromosome. The bacterium usually resides in soil or water but increasingly is found in
nosocomial infections where antibiotic resistance is increasing. Porins in the outer cell
membrane of the bacterium are greatly reduced in size compared to other Gram-negative
species and contribute to increased antibiotic resistance.

A. baumannii has been found to be the cause of up to 10% of Gram-negative bacterial
infections in Europe and the US. Individuals with weakened immune systems are
particularly susceptible to infection by this species. Due to the ability of the bacterium to
proliferate on many surfaces and environments including dry and static surfaces,
nosocomial infections may occur by patient contact with hospital equipment, especially
invasive apparatus where the bacterium can easily be introduced into the blood stream.

A. baumanni infections are known to be involved in respiratory and urinary tract infections
as well as causing pneumonia, septicaemia and meningitis.

Introduction to Acinetobacter baumannii

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MIN

MAX

The primers and probe sequences in this kit have 100% homology with a broad range of
clinically relevant reference sequences based on a comprehensive bioinformatics
analysis.

The PCR Max qPCR Kit for Acinetobacter baumannii (A.baumannii) genomes is designed
for the in vitro quantification of A.baumannii genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the A.baumannii genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
A.baumannii sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• A.baumannii specific primer/probe mix (150 reactions BROWN)

FAM labelled

• A.baumannii positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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