Instruction Sheet
for the PASCO
Model CI-6507A
pH electrode
012-06832A
06/98
$1.00
pH Sensor
pH electrode amplifier
cable with DIN
connectors
Connect to a
ScienceWorkshop
interface box
soaker bottle with
buffer solution
Introduction
The PASCO CI-6507A pH Sensor (pH electrode and
amplifier) is designed to be used with PASCO
ScienceWorkshop computer interface systems (300,
500, 700, and 750).
The pH electrode connects to the amplifier box with a
BNC connector, and the amplifier plugs into the
interface box directly or via a connecting cable.
The pH electrode is a standard type, producing an
electrical potential difference depending on the
hydrogen ion concentration (potential of hydrogen) in
a solution. The pH electrode is stored in a small plastic
container that contains a pH 4 buffer solution.
The pH amplifier translates the potential differences
measured by the pH electrode into the voltages
required by the Science Workshop interface. The
amplifier allows any standard pH electrode to be used
with ScienceWorkshop.
Equipment
Included:
PASCO CI-6507A pH amplifier
pH electrode with soaker bottle
connecting cable
soaker bottle containing KCl buffer solution (pH 4)
Additional Equipment Required:
ScienceWorkshop 300, 500, 700, or 750 Interface
Additional Material Required
distilled water (for rinsing the pH electrode)
buffer solutions, such as pH 4 and pH 7 (for
calibration of the electrode)
© 1998 PASCO scientific
pH Sensor 012-06832A
Using the pH Sensor
Calibrating the pH Sensor
Connecting the pH Sensor to the interface box
1. Connect the 8-pin DIN plug of the pH Sensor
amplifier box into analog channel A, B or C of
the computer interface (Figure 1a).
or
Use the supplied cable to connect the pH
electrode amplifier box to the analog channel of
the interface box (Figure 1b).
ab
ISE
Plug into analog
channel A, B, or C
to analog channel
on the computer
interface
Figure 1
Connecting the pH electrode amplifier to the computer
interface (two methods)
2. Unscrew and remove the protective soaker bottle
from the end of the pH electrode, leaving the Oring and cap in place on the shaft of the pH
electrode.
3. Rinse the electrode tip in distilled water. If
bubbles are seen in the pH electrode bulb, gently
shake the electrode downward (similar to
shaking down a thermometer) until the bubbles
disappear.
4. Connect the pH electrode to the BNC connector
of the pH electrode amplifier (Figure 2). Insert
the plug at the end of the electrode cable over the
BNC connector, and twist the plug one quarter
turn clockwise to lock it onto the BNC
connector.
Two Buffer Method:
One Buffer Method:
➤ Note: Use buffer solutions that are at a
temperature similar to the temperature of the
sample to be measured.
1. Run the ScienceWorkshop program. In the
Experiment Setup window, set up the
ScienceWorkshop interface and sensor so the pH
Sensor is connected into the correct analog
channel of the interface.
2. In the Experiment Setup window, double-click
the pH Sensor icon to open the sensors setup
window. (Note: In ScienceWorkshop version 3,
click the Calibration tab located near the top of
the sensors setup window.
3. Place the pH electrode in a high pH buffer
solution and wait about 30 seconds for the
voltage readings to stabilize.
4. Once the readings have stabilized, click the Take
Reading button. Enter the pH value of the high
pH buffer solution in the High Value box.
5. Rinse the pH electrode with distilled water and
then place the pH electrode in a second solution,
such as a low pH buffer solution. Wait about 30
seconds for the voltage readings to stabilize.
Again, after the readings have stabilized, click
the Take Reading button. Enter the pH value of
the low pH buffer solution in the Low Value
box. Click OK to return to the Experiment Setup
window.
1. Choose a buffer of known pH that is close to the
pH of the unknown.
2. Place the electrode in the buffer solution and
wait about 30 seconds for the voltage readings
stabilize.
Figure 2
Connecting the pH electrode to the amplifier
3. Type the pH of the buffer solution in the High
Value box, and click Take Reading.
4. Type 0 in the Low Value box, and type 0 in the
Volts box next to the Low Value box.
2