Pall 5017, 5015, 5014, 5016 Instruction Manual

Multi-Well Plate Vacuum Manifold

Description

The Multi-well Plate vacuum manifold is an anodized aluminum manifold that has
been designed and optimized for the vacuum filtration of the AcroPrep

™

AcroWell
with the necessary O-ring and gasket. The control block includes the vacuum
pressure gauge, vacuum metering valve, vacuum release valve and the 1/4 inch
hose barb for vacuum line attachment. Included with the vacuum manifold unit is a
Delrin spacer block designed to accommodate standard 350 µL receiver plates.
The spacer block has been optimized to reduce the space between the receiver
plate and the filter plate during vacuum filtration. A spacer block that has been
optimized to work with our 1 mL plate is available separately. An accessory kit that
contains an additional O-ring, gasket and allen wrench is also included with the
vacuum manifold unit.

lines of multi-well filter plates. The vacuum manifold comes complete

Ordering Information

Prod. No. Description Packaging

5017 Multi-well Plate Vacuum Manifold 1/pkg
5014 1 mL Receiver Plate Spacer Block 1/pkg
5015 350 µL Receiver Plate Spacer Block 1/pkg
5016 Replacement Accessory Kit (Includes O-Ring, 1/pkg

Note: the 350 µL Receiver Plate Spacer Block can be used to accomodate 384
well receiver plates as well.

gasket and allen wrench)

Specifications

Materials of Construction

Vacuum Manifold: Anodized aluminum
Gasket: EDPM (Ethylene propylene)
O-ring: Silicone
Spacer blocks: Delrin

Dimensions

Length: 17.48 cm (6.88 in)
Width: 12.37 cm (4.87 in)
Height: 8.05 cm (3.17 in)
Weight: 6.27 lbs

Maximum Operating Vacuum

71.12 cm Hg (28 in Hg).
Note: The multi-well plate vacuum manifold can be used with multi-well filter plates

that meet the specifications set forth by the Society for Biomolecular Screening
(SBS).

Instructions for Use

To set the vacuum manifold to a desired negative pressure setting prior to filtration,
follow the steps listed below.

11. Switch the on/off valve to the off position (See Figure 3).

12. Attach the vacuum line to the 1/4 inch hose barb.

13. Place a receiver plate on top of the vacuum manifold.

14. Turn on the vacuum pressure. You may need to press lightly on the receiver

plate to engage the vacuum seal.

15. Adjust the negative pressure to the desired setting by adjusting the metering

valve.

16. Turn off the vacuum pressure and remove the receiver plate. The vacuum

manifold is now set at the desired setting.

To filter the AcroPrep or AcroWell filter plates follow the steps listed below.

11. If collecting the filtrate, remove the top chamber of the vacuum manifold. The

indentations on the sides of the manifold allow for the top portion of the
manifold to be easily removed. If collecting the retentate, skip to step 3.

12. Place the appropriate spacer block into the lower chamber of the vacuum

manifold. The vacuum manifold can be used with any size receiver plate.
350 µL receiver plates: To use a 350 µL 96 well or 384 well receiver plate,

place the large spacer block (PN 5015) into the lower chamber of the vacuum
manifold. Place the 350 µL 96 well or 384 well receiver plate on top of the
spacer block.

1 mL receiver plates: To use 1 mL receiver plates, place the small spacer block
(PN 5014) into the lower chamber of the vacuum manifold. Place the 1 mL
receiver plate on top of the spacer block. (You can also use a standard 350 µL
receiver plate in place of the 1 mL spacer block.)

2 mL receiver plates: To use 2 mL receiver plates, place the 2 mL receiver plate
directly into the lower chamber of the vacuum manifold.

™

and

Instructions for Use (cont.)

13. Before replacing the top chamber of the vacuum manifold, ensure that all

surfaces are free from dirt, debris and any particulate matter that may have
accumulated on the vacuum manifold, O-ring and gasket.

Note: If the O-ring and gasket are not clean, you will not obtain a proper seal.

14. Replace the top chamber of the vacuum manifold.

15. Place the filter plate on the gasket located on the top chamber of the vacuum

manifold. Ensure that the gasket is clean.

16. Ensure that the on/off switch is in the position (See Figure 3).

17. Connect the vacuum line to the 1/4 inch hose barb.

18. When ready to evacuate the filter plate, turn the on/off switch on the vacuum

manifold to the on position. You may need to press lightly on the filter plate to
engage the vacuum seal.

19. The wells will begin to evacuate/empty once the vacuum has been applied to

the chamber.

10. If you need to adjust the vacuum pressure up or down, adjust by moving the
metering valve located to the right of the vacuum pressure gauge.

11. When all of the wells have completely evacuated, turn the on/off valve to the off
position. To release the residual vacuum pressure that remains in the chamber,
push the release valve located to the left of the vacuum gauge. The release
valve will allow the pressure within the manifold chamber to return to
atmospheric pressure and reduce the potential for cross contamination and
spraying of the filtrate. Do not release the vacuum by pulling the corner of
the plate as it will degrade the manifold gasket. You can also tap the top of
the filter plate prior to removing it to release any hanging drops that may be
attached to the outlet tips.

12. Remove the filter plate and place it aside for further processing or dispose of
properly.

13. If collecting the filtrate, remove the top chamber of the vacuum manifold from
the lower portion of the vacuum manifold.

14. Remove the receiver plate from the lower chamber of the vacuum manifold and
utilize the filtrate for further processing.

To r eplace the O-ring follow the steps below.

11. Remove existing O-ring from the bottom of the upper chamber.

12. Ensure that the new O-ring and O-ring groove are free from dirt, debris and

particulate matter.

13. Place new O-ring into place.

To r eplace the gasket follow the steps below.

11. With the allen wrench provided, remove the 12 screws located on the bottom

portion of the upper chamber.

12. Separate the top ring from the bottom section of the upper chamber.

13. Remove the old gasket and clean the gasket pocket area of any dirt and

debris.

14. Place the new gasket into the gasket pocket. Place the top ring back onto the

bottom section of the upper chamber. Ensure that the alignment posts on the
bottom section fit into the alignment holes in the top ring.

15. Invert the upper chamber and replace the 12 screws.

16. Lightly tighten the four corner screws with the allen wrench, then tighten all 12

screws. Once this is completed recheck the screws with the allen wrench to
ensure all screws are tightly secured.

17. The manifold is now ready for use.

Figure 1

Gasket

1/4 inch hose barb

On/Off switch

Vacuum metering valve

Vacuum pressure gauge

Figure 1

O-ring

350 µL spacer block

Control block

Vacuum release valve

Instructions for Use (cont.)

Figure 2Figure 2

gasket

1 mL receiver
plate

1 mL receiver plate
spacer block (PN 5014)

2 mL receiver
plate

350 µL receiver

plate

350 µL receiver plate

spacer block (PN 5015)

Lower chamber

Upper chamber

Figure 2

Note: A 384 well receiver plate can be interchanged with the 350 µL receiver plate
if using a 384 well filter plate.

On/Off valve

On position

Complementary Products

™

AcroWell

Membranes exhibit high binding capacities for proteins and nucleic acids.

AcroPrep

variety of molecular biology, analytical and high throughput sample preparation and
detection applications.

96-well Filter Plates with BioTrace™NT and BioTrace PVDF

™

96 and 384-well Filter Plates are an excellent platform for a wide

WARNING

Employment of the products in applications not specified, or failure to follow
all instructions contained in this product information insert, may result in
improper functioning of the product, personal injury, or damage to property
or the product. See Statement of Warranty in our most recent catalog.

Figure 3

Vacuum relief valve,

push down on button

to activate.

Off position

Metering valve fully open when
knob is turned completely down.
Turn counter clockwise to lower
vacuum (minimum vacuum
setting is 3" Hg).

Pall Life Sciences

600 South Wagner Road
Ann Arbor, MI 48103-9019 USA

For ordering or technical information:
Tel: [phone]
800-521-1520 (in USA)
Fax: [phone]

Visit Pall Life Sciences on the
Internet: www.pall.com/Lab or
e-mail us at: [e-mail]

Offices:

Australia, Lane Cove, NSW, 02-9428 2333
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Pall, , AcroPrep, AcroWell, and BioTrace are trademarks of Pall Corporation.

® indicates a registered trademark in the USA.

©2003, Pall Corporation, 09/03 PN 88293A