Oroboros Instruments O2k User Manual

Oroboros Instruments www.oroboros.at

Oroboros O2k-Manual

Mitochondrial Physiology Network 07.08(09):1-7 (2017)

Version 09: 2017-11-16 O2k ©2010-2017 Oroboros

Updates: http://wiki.oroboros.at/index.php/O2k-Manual

O2k-Manual:
user information

1

Oroboros Instruments

High-Resolution Respirometry
Schöpfstrasse 18, A-6020 Innsbruck, Austria
Email: [e-mail]

www.oroboros.at

Section ..............................................................................................

1. About the O2k-Manual ............................................................... 1

2. General introduction .................................................................. 2

3. Scope of application .................................................................. 2

4. Warranty.................................................................................. 2

5. Important safety information ..................................................... 3

5.1. Technical requirements for safe operation .................................................................................. 3

5.2. Qualified personnel ...................................................................................................................... 3

5.3. Safety symbols and terms ............................................................................................................ 4

6. Electrostatic discharge (ESD): Damage and protection ................... 4

7. Maintenance ............................................................................. 6

8. Disclaimer ................................................................................ 6

9. Insurances ............................................................................... 6

10. Limitation of liability .................................................................. 6

11. Copyright and software license ................................................... 6

12. International O2k-Workshops ..................................................... 6

13. Cooperation and feedback in science ........................................... 7

PLEASE READ THIS MANUAL CAREFULLY BEFORE ATTEMPTING TO USE THIS
INSTRUMENT. The Oroboros O2k, O2k-MultiSensor Modules, Titration­Injection microPump (TIP2k) and Oroboros DatLab software constitute a
modular integrated scientific equipment, collectively abbreviated as the O2k­System or O2k.

1. About the O2k-Manual

This document provides the users of the Oroboros O2k system with
instructions for setting up the instrument, performing experiments and

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evaluation data with the O2k system. This manual comprises different
chapters that may be available (printed or in electronic form) together or
seperately. The term manual as used herein refers to all documents available
as printed material, or provided on the USB flash drive ‘Oroboros O2k-Course
on HRFR’, and updated on the websites of Oroboros Instruments.

» www.oroboros.at

» http://wiki.oroboros.at/index.php/OROBOROS_info

O2k-Manual:
»wiki.oroboros.at/index.php/MiPNet22.11_O2k-FluoRespirometer_manual

2. General introduction

The O2k-Manual describes the safe and appropriate handling of the O2k. The
mentioned safety indications and instructions as well as the safety regulations
have to be followed.

Before starting to operate the machine, every person going to handle
the O2k has to read the O2k-User manual carefully and get used to the
operation of the device before using the instrument. This is also true for
persons already working with similar machines or persons who have received
an introduction to the instrument by the producer.
The knowledge of the content of the O2k-User Manual is required to
protect the employees from hazards as well as to avoid mistakes to guarantee
safe and undisturbed operation of the instrument.

The O2k-User manual is an integral part of the O2k and should be kept
near the machine to be available to every person who is going to work with
the machine in case of doubt. The instruction manual has to be delivered to
all persons working with the O2k.

3. Scope of application

The O2k-System is not intended for in vivo applications, except for
considering living cells as in vivo models. In particular, the TIP2k is not to be
used for infusion on humans and live animals.

The O2k is tested in a wide range of measurements in vitro, with
particular emphasis on high-resolution detection of changes of oxygen
concentration in the O2k-Chambers. The TIP2k is intended for automatic
titrations and injections into the O2k. For applications beyond this scope of
applications and without following the necessary instructions and safety
precautions, no warranty is granted and no responsibility is assumed by
Oroboros Instruments GmbH (Corp).

Applications of the Oroboros O2k beyond the scope of applications
described above are not allowed and are classified as not in accordance with
the regulations.

4. Warranty

The company Oroboros Instruments warrants the appropriate function of the
O2k-System within the limits of specifications, if no manipulations are
effected on the instrument (mechanics, electronics and DatLab software), if
the specified types of PC, voltage, and syringes are used, and all instructions

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of the operation manual (O2k-Manual) are observed. These include
instructions and warnings, adherence to which is essential for proper function
and secure conditions of the O2k-System.
While every reasonable effort has been made in the preparation of this
manual, Oroboros Instruments cannot assume any responsibility for errors or
omissions. Neither is any liability assumed for damages resulting from the use
of the information contained in this document.
DatLab Limited Warranty: If you report, in writing, a significant
defect in the DatLab software which cannot be corrected within 90 days of the
date of the report, you may return the program and be refunded the purchase
price of the program. Defective USB flash drives will be replaced at no charge
within 90 days of the date of purchase.

5. Important safety information

To guarantee safe operation of the O2k-System, it is absolutely necessary to
follow the instructions of the O2k-Manual.
Prior to start of operation, take notice of the instructions for installation
and operating instructions. Furthermore, check the technical intactness as
well as the operating reliability of the machine before starting the instrument.
The mentioned safety indications and instructions of the O2k-User
Manual may be followed to avoid injury of employees as well as damage to
property when handling the maschine. Nonobservance of these indications
and instructions can endanger employees and damage or destroy the
instrument.
Noncompliance of the safety indications and instructions of this user
manual as well as the accident prevention regulations and safety regulations
of the scope of application will eliminate the claim for liability and damage
towards the producer.

5.1. Technical requirements for safe operation

The O2k is built according to the safety regulations for electronic instruments,
control devices and laboratory equipment and is operationally reliable as long
as no manipulations are effected on the instrument (mechanics, electronics
and DatLab software). Furthermore, the operating safety is only ensured
under the intended usage of the instrument.
The accessories used in conjunction with the O2k must be those
specified or delivered by Oroboros Instruments, in particular the syringes for
the TIP2k and injection needles which have to be maintained clean. For the
control with a computer, a registered version of the Oroboros software DatLab
must be used, else no warranty will be given for the specified quality of
performance.
During application of the O2k, any condition of the instrument which
could lead to damage of material or respiratory irritation of humans must be
avoided. The instrument can only be operated with the components shipped
with the O2k.

5.2. Qualified personnel

The O2k can be hazardous for untrained persons due to inappropriate
handling which is not in accordance with the regulations of the machine.
The chapters of this O2k-User Manual comprise precise safety
indications, marked with specific symbols, to avoid hazards to all persons
dealing with the O2k. The O2k described in this documentation may be
operated only by personnel qualified for the specific task in accordance with

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the relevant documentation for the specific task, in particular its warning
notices and safety instructions. Qualified personnel are those who,

a) based on their training and experience, are capable of identifying risks and

avoiding potential hazards when working in a labarory environment in general
and with the described system in particular;

b) are able to read and understand this manual (which implies a working

knowledge of English);

c) have familiarized themselves with this manual and the safety precautions

outlined in it.
The appropriate handling of the instrument includes the observation of
the conditions of operation as well as the information and instructions
included in the O2k-User Manual.

5.3. Safety symbols and terms

WARNING with a safety alert symbol: explains dangers that could result in
severe personal injury or death if proper precautions are not taken.

CAUTION with a safety alert symbol: explains hazards that could result in
minor personal injury if proper precautions are not taken.

CAUTION without a safety alert symbol: explains hazards that may result in
damage to the instrument or other property.

CAUTION: Electrostatic discharge may result in damage to electronic

equipment: Observe general electrostatic discharge (ESD) protection
procedures.

Warning: Wear safety goggles! This symbol indicates that safety goggles
have to be worn to avoid a specific hazard of the O2k system. Independent of
the O2k system, general laboratory safety procedure that may require to wear
safety goggles have to be observed at all times.

6. Electrostatic discharge (ESD): Damage and protection

What is ESD? General electrostatic discharge (ESD)

protection procedures should be observed when handling
any kind of electronic equipment, including the
OROBOROS Oxygraph-2k. While just slightly unpleasant
or even unrecognizable to us, ESD sparks may cause
severe damage to integrated circuits and other electronic
devices.
The best known experience of an ESD is feeling a
spark when touching some object or another person. ESD
is the sudden and momentary electric current that flows between two objects
at different electrical potentials. Such a potential difference may commonly
build up by

• Walking across the floor,

• Handling packaging material such as styrofoam and other plastic materials,

• Removing adhesive tape from a roll or container,

• Transporting equipment on trays or carts,

• Sliding items on a work bench.

More details: http://en.wikipedia.org/wiki/Electrostatic_discharge

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Potential Damage of the Oxygraph-2k by ESD

Within the stainless steel Oxygraph-2k housing, internal electronic
components are well protected from ESD. However, during chamber
assembly, membrane change, or trouble shouting, the polarographic
oxygen sensor (POS) has to be disconnected from the POS connector
and the POS connector is disconnected from the main unit. This
means that one or both electric connections of the POS connector
are exposed to the environment and ESD damage is possible.
During such operations special care must be taken to observe
ESD prevention procedures.

For oxygraphs with MultiSensor extension the additional
channels by definition open up additional routes how an ESD
discharge can reach the inside of the oxygraph. BNC type connectors

are used frequently for attaching potentiometric electrodes (pH) and
therefore also used for the pX channel in the MultiSensor extension.
By the nature of the BNC plug it is possible for the operator to touch
the outside channel and thereby cause an ESD.

ESD Protection Procedures: The simplest and most effective way to

prevent damage by ESD is to get rid of your electric charge before handling
the POS connector, the BNC plug of the pX channel or other electronic
connections. Preventive ESD is achieved simply by touching a well grounded
object, such as the stainless steel housing of the O2k. Touching the O2k
housing does not cause any damage, and should be done before handling the
electrical connectors. This will be sufficient in most cases to prevent damage.
Leaving POC connectors attached to the main unit while cleaning or
servicing them and while membrane mounting will, to a certain degree, also
protect the electronics inside from damage by ESD. Protecting POS connectors
from ESD is especially important for oxygraphs Series A to C, because more
electronic modules are in these connectors.
Do not touch the BNC plug of oxygraphs equipped with a MultiSensor
extension, unless necessary. Hypothetically an ESD might also transmitted via
the pX channel by touching the needles of the TIP syringes while a electrode
in the chamber is connected to the pX electronics. Therefore the oxygraph
housing should be touched before touching the TIP syringes when working
with an electrode attached to the pX channel (pH, TPP+, Ca2+).

For oxygraph Series A to C requiring a MultiSensor Conntector, the
guidelines also apply to the BNC port on the MultiSensor Connector.
It is general good laboratory practice to avoid excessive build­up of electrostatic charges. If you experience frequent sparks while
touching objects or other persons in your lab, it might be a good
idea to consult an electronic expert in your institution, to protect all

kind of electronic equipment with preventive measures. The magnitude of the
ESD problem depends strongly on conditions in a lab, such as floor material or
humidity on one hand and clothing - especially footwear - on the other.
A special topic are the shoes known as “Crocs” and similar products.
With these shoes it is possible to generate extremely high potentials. In some
hospitals such shoes have already been banned, because they apparently
destroyed a lot of expensive equipment. Therefore, such shoes should not be
worn in any laboratory.

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7. Maintenance

Maintenance and repairs may be carried out only by qualified personnel.
Repairs beyond the maintenance described in the O2k-Manual may be carried
out only by the producer or service authorized by Oroboros Instruments. The
stainless steel instrument housing must not be opened, otherwise any
warranty claims will be rejected.

8. Disclaimer

The O2k is a tool intended to be used by trained professionals only. The
DatLab software is not a substitute for your personal professional judgement.
This software has been carefully designed. In such an elaborate computer
program, defects cannot be completely ruled out. Under no circumstances
may this software be used for tasks involving human safety. Persons using
this software are responsible for the supervision, management, and control of
this software. This responsibility includes, but is not limited to, the
establishment of independent procedures for testing the reliability and
accuracy of any program output.

9. Insurances

Oroboros Instruments GmbH holds product and public liability insurances with
worldwide coverage.

10. Limitation of liability

The manufacturer and distributor of this software will not be held liable for
damages including loss of data, lost profits, or other special, incidental or
consequential or indirect damages arising from the use or inability to use the
O2k.

11. Copyright and software license

All rights reserved. Neither this manual nor any part of the Oroboros USB
Flash Drive may be reproduced or transmitted in any form or by any means,
electronic or mechanical, including photocopying, microfilming and recording,
or by any information storage and retrieval system, without written
permission from Oroboros Instruments.

All rights to the Oroboros DatLab program, including the rights to copy,
distribute, and translate this software remain with Oroboros® Instruments
GmbH, Innsbruck, Austria. You may make copies of DatLab for Acquisition
and Analysis of data related to your O2k. You may not make copies of the
program or of any file of the Oroboros USB Flash Drive or from the Oroboros
website for the distribution, lease or sale to another party.

12. International O2k-Workshops

Although the O2k-Manual provides necessary and sufficient information on
application of the O2k-System, it is highly recommended to register for an
International O2k-Workshop organized by Oroboros Instruments.

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13. Cooperation and feedback in science

Since product development and improvement is a continuous process,
Oroboros Instruments reserves the right to make changes in the specification
without notice.
O2k-Network Reference Laboratories build a WorldWide Network. O2k­Network Reference Laboratories may be contacted for local technical advice
on applications of the Oroboros O2k and High-Resolution FluoRespirometry.
The O2k-Network is an initiative for combining the expertise of various
laboratories to a joint development for expansion of the O2k into the O2k­MultiSensor System, from prototypes to series production.
MiPNet Application Notes and publications in scientific journals provide
information about current developments complementary to the O2k-Manual.
If you have any comments on the O2k and the O2k-Manual, we will be
pleased to receive them at:

Oroboros Instruments
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: [phone] | Fax: [phone] | [e-mail]

http://www.oroboros.at

©2007-2017 Oroboros® Instruments GmbH.
Printed in Austria. Subject to change without prior notice.

Oroboros O2k: Development – Production - Distribution

Oroboros Instruments, Schöpfstrasse. 18, A-6020 Innsbruck, Austria;

[e-mail]

Development of O2k High-Resolution FluoRespirometry in collaboration with:

• Philipp Gradl, WGT-Elektronik GmbH & Co KG, A-6114 Kolsass/Tirol, Austria.

Electromechanics development and production of the Oroboros O2k, TIP2k,

ISS, and polarographic oxygen sensor (OroboPOS).

• Lukas Gradl, software security networks, A-6020 Innsbruck, Austria.

Software development: DatLab.

O2k-WorldWide - User innovation – the O2k-Network
» www.oroboros.at