Oroboros Instruments O2k User Manual

Oroboros Instruments www.oroboros.at

Oroboros O2k-Manual

Mitochondrial Physiology Network 07.08(09):1-7 (2017)

Version 09: 2017-11-16 O2k ©2010-2017 Oroboros

Updates: http://wiki.oroboros.at/index.php/O2k-Manual

O2k-Manual:
user information

1

Oroboros Instruments

High-Resolution Respirometry
Schöpfstrasse 18, A-6020 Innsbruck, Austria
Email: [e-mail]

www.oroboros.at

Section ..............................................................................................

1. About the O2k-Manual ............................................................... 1

2. General introduction .................................................................. 2

3. Scope of application .................................................................. 2

4. Warranty.................................................................................. 2

5. Important safety information ..................................................... 3

5.1. Technical requirements for safe operation .................................................................................. 3

5.2. Qualified personnel ...................................................................................................................... 3

5.3. Safety symbols and terms ............................................................................................................ 4

6. Electrostatic discharge (ESD): Damage and protection ................... 4

7. Maintenance ............................................................................. 6

8. Disclaimer ................................................................................ 6

9. Insurances ............................................................................... 6

10. Limitation of liability .................................................................. 6

11. Copyright and software license ................................................... 6

12. International O2k-Workshops ..................................................... 6

13. Cooperation and feedback in science ........................................... 7

PLEASE READ THIS MANUAL CAREFULLY BEFORE ATTEMPTING TO USE THIS
INSTRUMENT. The Oroboros O2k, O2k-MultiSensor Modules, Titration­Injection microPump (TIP2k) and Oroboros DatLab software constitute a
modular integrated scientific equipment, collectively abbreviated as the O2k­System or O2k.

1. About the O2k-Manual

This document provides the users of the Oroboros O2k system with
instructions for setting up the instrument, performing experiments and

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evaluation data with the O2k system. This manual comprises different
chapters that may be available (printed or in electronic form) together or
seperately. The term manual as used herein refers to all documents available
as printed material, or provided on the USB flash drive ‘Oroboros O2k-Course
on HRFR’, and updated on the websites of Oroboros Instruments.

» www.oroboros.at

» http://wiki.oroboros.at/index.php/OROBOROS_info

O2k-Manual:
»wiki.oroboros.at/index.php/MiPNet22.11_O2k-FluoRespirometer_manual

2. General introduction

The O2k-Manual describes the safe and appropriate handling of the O2k. The
mentioned safety indications and instructions as well as the safety regulations
have to be followed.

Before starting to operate the machine, every person going to handle
the O2k has to read the O2k-User manual carefully and get used to the
operation of the device before using the instrument. This is also true for
persons already working with similar machines or persons who have received
an introduction to the instrument by the producer.
The knowledge of the content of the O2k-User Manual is required to
protect the employees from hazards as well as to avoid mistakes to guarantee
safe and undisturbed operation of the instrument.

The O2k-User manual is an integral part of the O2k and should be kept
near the machine to be available to every person who is going to work with
the machine in case of doubt. The instruction manual has to be delivered to
all persons working with the O2k.

3. Scope of application

The O2k-System is not intended for in vivo applications, except for
considering living cells as in vivo models. In particular, the TIP2k is not to be
used for infusion on humans and live animals.

The O2k is tested in a wide range of measurements in vitro, with
particular emphasis on high-resolution detection of changes of oxygen
concentration in the O2k-Chambers. The TIP2k is intended for automatic
titrations and injections into the O2k. For applications beyond this scope of
applications and without following the necessary instructions and safety
precautions, no warranty is granted and no responsibility is assumed by
Oroboros Instruments GmbH (Corp).

Applications of the Oroboros O2k beyond the scope of applications
described above are not allowed and are classified as not in accordance with
the regulations.

4. Warranty

The company Oroboros Instruments warrants the appropriate function of the
O2k-System within the limits of specifications, if no manipulations are
effected on the instrument (mechanics, electronics and DatLab software), if
the specified types of PC, voltage, and syringes are used, and all instructions

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Oroboros Instruments Oroboros O2k-Manual

of the operation manual (O2k-Manual) are observed. These include
instructions and warnings, adherence to which is essential for proper function
and secure conditions of the O2k-System.
While every reasonable effort has been made in the preparation of this
manual, Oroboros Instruments cannot assume any responsibility for errors or
omissions. Neither is any liability assumed for damages resulting from the use
of the information contained in this document.
DatLab Limited Warranty: If you report, in writing, a significant
defect in the DatLab software which cannot be corrected within 90 days of the
date of the report, you may return the program and be refunded the purchase
price of the program. Defective USB flash drives will be replaced at no charge
within 90 days of the date of purchase.

5. Important safety information

To guarantee safe operation of the O2k-System, it is absolutely necessary to
follow the instructions of the O2k-Manual.
Prior to start of operation, take notice of the instructions for installation
and operating instructions. Furthermore, check the technical intactness as
well as the operating reliability of the machine before starting the instrument.
The mentioned safety indications and instructions of the O2k-User
Manual may be followed to avoid injury of employees as well as damage to
property when handling the maschine. Nonobservance of these indications
and instructions can endanger employees and damage or destroy the
instrument.
Noncompliance of the safety indications and instructions of this user
manual as well as the accident prevention regulations and safety regulations
of the scope of application will eliminate the claim for liability and damage
towards the producer.

5.1. Technical requirements for safe operation

The O2k is built according to the safety regulations for electronic instruments,
control devices and laboratory equipment and is operationally reliable as long
as no manipulations are effected on the instrument (mechanics, electronics
and DatLab software). Furthermore, the operating safety is only ensured
under the intended usage of the instrument.
The accessories used in conjunction with the O2k must be those
specified or delivered by Oroboros Instruments, in particular the syringes for
the TIP2k and injection needles which have to be maintained clean. For the
control with a computer, a registered version of the Oroboros software DatLab
must be used, else no warranty will be given for the specified quality of
performance.
During application of the O2k, any condition of the instrument which
could lead to damage of material or respiratory irritation of humans must be
avoided. The instrument can only be operated with the components shipped
with the O2k.

5.2. Qualified personnel

The O2k can be hazardous for untrained persons due to inappropriate
handling which is not in accordance with the regulations of the machine.
The chapters of this O2k-User Manual comprise precise safety
indications, marked with specific symbols, to avoid hazards to all persons
dealing with the O2k. The O2k described in this documentation may be
operated only by personnel qualified for the specific task in accordance with