Oritron ICS-2100 User Manual
ICS-2100 Ion Chromatography System
Operator’s Manual
Document No. 065291
Revision 01
March 2009
©2009 by Dionex Corporation
All rights reserved worldwide.
Printed in the United States of America.
This publication is protected by federal copyright law. No part of this publication
may be copied or distributed, transmitte d, tr anscribed, stored in a retrieval system, or
transmitted into any human or computer language, in any form or by any means,
electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties
without the express written permission of Dione x Corporation , 1228 Titan Way,
Sunnyvale, California 94088-3603 U.S.A.
DISCLAIMER OF WARRANTY AND LIMITED WARRANTY
THIS PUBLICATION IS PROVIDED “AS IS” WITHOUT WARRANTY OF
ANY KIND. DIONEX CORPORATION DOES NOT WARRANT,
GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED
REPRESENTATIONS REGARDING THE USE, OR THE RESULTS OF T HE
USE, OF THIS PUBLICA TION IN TERMS OF CORRECTNESS, ACCURACY,
RELIABILITY, CURRENTNESS, OR OTHERWISE. FURTHER, DIONEX
CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION
AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT
HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO
NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR
CHANGES.
TRADEMARKS
AES, AMMS ICE, ASRS, Chromeleon, EluGen, IonPac, OnGuard, and SRS are
registered trademarks of Dionex Corporation. AutoNeutraliz ation, MicroMembran e,
MMS, and MonoDisk are trademarks of Dionex Corporation.
Acrobat, Adobe, and Adobe Reader are registered trademarks of Adobe Systems,
Incorporated.
Microsoft and Windows Vista are registered trademarks of Microsoft Corporation.
PEEK is a trademark of Victrex PLC.
Teflon is a registered trademark of E.I. duPont de Nemours & Company.
PRINTING HISTORY
Revision 01, March 2009
Contents
1 • Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 Introduction to Ion Chromatography (IC) . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 Overview of the ICS-2100 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.3 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3.1 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Safety and Regulatory Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.4.1 Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 • Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1 Operating Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.1 Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.2 Top Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.1.3 Component Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.1.4 Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
2.2 Flow Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
2.3 Chromeleon and Chromeleon Xpress . . . . . . . . . . . . . . . . . . . . . . . . . . 30
2.3.1 The Panel Tabset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
2.3.2 Software Control Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
2.3.3 System Wellness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
2.4 System Component Details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
2.4.1 Vacuum Degas Assembly (Optional) . . . . . . . . . . . . . . . . . . . . 32
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ICS-2100 Ion Chromatography System
2.4.2 Eluent Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
2.4.3 Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
2.4.4 Eluent Generator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
2.4.5 Auxiliary Power Supply (Optional) . . . . . . . . . . . . . . . . . . . . . .42
2.4.6 Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42
2.4.7 Auxiliary Valve (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
2.4.8 Column Heater . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
2.4.9 Suppressor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
2.4.10 DS6 Heated Conductivity Cell . . . . . . . . . . . . . . . . . . . . . . . . . 46
3 • Operation and Maintenance . . . . . . . . . . . . . . . . . . . . . . . .49
3.1 Operation Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
3.2 Turning On the System Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
3.3 Connecting to Chromeleon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
3.4 Set Up the Eluent Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
3.4.1 Filter the Deionized Water . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
3.4.2 Fill the Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
3.4.3 Set the Eluent Level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
3.4.4 Connect the Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56
3.5 Check All Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
3.6 Prime the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
3.7 Set System Operating Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
3.8 Equilibrate the System and Verify Operational Status . . . . . . . . . . . . .59
ii Doc. 065291-01 3/09
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3.9 Prepare Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
3.9.1 Collecting and Storing Samples . . . . . . . . . . . . . . . . . . . . . . . . 60
3.9.2 Pretreating Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
3.9.3 Diluting Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
3.10 Loading and Injecting Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
3.10.1 Loading Samples with a Syringe . . . . . . . . . . . . . . . . . . . . . . . 63
3.10.2 Loading Samples with a Vacuum Syringe . . . . . . . . . . . . . . . . 64
3.10.3 Loading Samples with an Autosampler . . . . . . . . . . . . . . . . . . 64
3.10.4 Injecting Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
3.11 Processing Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
3.11.1 Manual Sample Processing . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
3.11.2 Automatic (Batch) Sample Processing . . . . . . . . . . . . . . . . . . . 66
3.12 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
4 • Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
4.1 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
4.2 Troubleshooting Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
4.3 Liquid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
4.4 Pump Difficult to Prime or Loses Prime . . . . . . . . . . . . . . . . . . . . . . . . 88
4.5 Pump Does Not Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
4.6 No Flow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
4.7 Erratic Flow/Pressure Reading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
4.8 Excessive System Backpressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
4.9 Peak “Ghosting” . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
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ICS-2100 Ion Chromatography System
4.10 Nonreproducible Peak Height or Retention Time . . . . . . . . . . . . . . . . .94
4.11 Abnormal Retention Time or Selectivity . . . . . . . . . . . . . . . . . . . . . . . .94
4.12 No Cell Response . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94
4.13 High Cell Output . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
4.14 Baseline Noise or Drift . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96
4.15 Vacuum Degas Assembly Does Not Run . . . . . . . . . . . . . . . . . . . . . . . .97
5•Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99
5.1 Diagnostic and Calibration Procedures . . . . . . . . . . . . . . . . . . . . . . . . .99
5.1.1 Chromeleon Wellness Panel Overview . . . . . . . . . . . . . . . . . .100
5.1.2 Diagnostic and Calibration Touch Screen Overview . . . . . . .102
5.1.3 Calibrating the Conductivity Cell . . . . . . . . . . . . . . . . . . . . . .103
5.1.4 Calibrating the Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . .106
5.1.5 Calibrating the Vacuum Degas Assembly . . . . . . . . . . . . . . . .108
5.2 Isolating a Restriction in the Liquid Lines . . . . . . . . . . . . . . . . . . . . . .109
5.3 Replacing Tubing and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .113
5.4 Rebuilding the Injection Valve or Auxiliary Valve . . . . . . . . . . . . . . .114
5.5 Replacing an Auxiliary Valve Pod . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
5.6 Cleaning and Replacing the Pump Check Valves . . . . . . . . . . . . . . . .118
5.7 Replacing a Pump Piston Seal and Piston Rinse Seal . . . . . . . . . . . . .120
5.8 Replacing a Pump Piston . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124
5.9 Replacing the Waste Valve or Priming Valve O-Ring . . . . . . . . . . . . .125
5.10 Replacing the Conductivity Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
5.11 Replacing the Suppressor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .130
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5.12 Replacing the Column Heater . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
5.13 Replacing the Column Heater Heat Exchanger . . . . . . . . . . . . . . . . . . 134
5.14 Replacing the Eluent Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
5.15 Replacing the Leak Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
5.16 Priming the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138
5.16.1 Priming the Eluent Lines with a Syringe . . . . . . . . . . . . . . . . 138
5.16.2 Priming with the Prime Button . . . . . . . . . . . . . . . . . . . . . . . . 140
5.17 Priming the Pump with Isopropyl Alcohol . . . . . . . . . . . . . . . . . . . . . 141
5.18 Changing Main Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
5.19 Replacing an EluGen Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
5.19.1 Replacing a KOH, LiOH, MSA, or NaOH
EluGen Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
5.19.2 Replacing a K2CO3 Cartridge . . . . . . . . . . . . . . . . . . . . . . . . 151
5.20 Replacing the CR-TC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
5.21 Replacing the EPM Electrolytic pH Modifier . . . . . . . . . . . . . . . . . . . 169
5.21.1 Recording the EPM Serial Number in Chromeleon . . . . . . . . 170
5.21.2 Plumbing the EPM for Hydrating and Conditioning . . . . . . . 171
5.21.3 Hydrating and Conditioning the EPM . . . . . . . . . . . . . . . . . . 173
5.22 Replacing the EGC CO3 Mixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174
5.22.1 Installing the New EGC CO3 Mixer . . . . . . . . . . . . . . . . . . . 174
5.22.2 Filling the EGC CO3 Mixer with Deionized Water . . . . . . . . 175
5.22.3 Filling the EGC CO3 Mixer with Eluent . . . . . . . . . . . . . . . . 178
5.23 Replacing the EGC Holder and Degas Assembly . . . . . . . . . . . . . . . . 185
5.23.1 Disconnecting and Removing the EluGen Cartridge . . . . . . . 185
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ICS-2100 Ion Chromatography System
5.23.2 Removing the CR-TC and Reinstalling it
in the New EGC Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . .188
5.23.3 Removing the EPM and Reinstalling it
in the New EGC Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . .190
5.23.4 Installing the New EGC Holder Without a CR-TC
or EPM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192
5.23.5 Reinstalling the EluGen Cartridge . . . . . . . . . . . . . . . . . . . . . .193
A • Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195
A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195
A.2 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195
A.3 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .196
A.4 Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .196
A.5 Analytical Pump and Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .196
A.6 Eluent Generation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .198
A.7 Detector Electronics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .200
A.8 Conductivity Cell with Heat Exchanger . . . . . . . . . . . . . . . . . . . . . . . .200
A.9 Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .202
A.10 Auxiliary Valve (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .202
A.11 Vacuum Degas Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .202
A.12 Column Heater . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .202
A.13 Auxiliary Power Supply (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . .202
A.14 Suppressors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .203
A.15 Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .204
A.16 System Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .204
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B • Touch Screen Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . 207
B.1 Using the Touch Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 207
B.2 Using the Touch Screen with Chromeleon . . . . . . . . . . . . . . . . . . . . . 210
B.3 Overview of ICS-2100 Touch Screen Pages . . . . . . . . . . . . . . . . . . . . 211
B.4 Home Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 212
B.4.1 Home Page Pump Controls . . . . . . . . . . . . . . . . . . . . . . . . . . 212
B.4.2 Home Page EGC Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . 214
B.4.3 Home Page Column Heater . . . . . . . . . . . . . . . . . . . . . . . . . . 214
B.4.4 Home Page Injection Valve Controls . . . . . . . . . . . . . . . . . . . 215
B.4.5 Home Page Detector Controls . . . . . . . . . . . . . . . . . . . . . . . . 215
B.4.6 Other Home Page Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . 216
B.5 Plot Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217
B.6 Status Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 218
B.6.1 Viewing Other Status Parameters . . . . . . . . . . . . . . . . . . . . . . 220
B.6.2 Status Parameter Details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221
B.7 Pump Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223
B.7.1 Setting Pump Pressure Limits and Selecting
the Pressure Unit 223
B.7.2 Setting Degas Operating Parameters (Optional) . . . . . . . . . . 224
B.7.3 Controlling the Eluent Valve . . . . . . . . . . . . . . . . . . . . . . . . . 225
B.8 Eluent Generator Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 226
B.8.1 EGC Serial Number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 226
B.8.2 Setting the Eluent Concentration . . . . . . . . . . . . . . . . . . . . . . 227
B.8.3 Controlling the Eluent Generator Power . . . . . . . . . . . . . . . . 228
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B.8.4 Monitoring the EluGen Cartridge Life . . . . . . . . . . . . . . . . . .228
B.8.5 Controlling the CR-TC Power . . . . . . . . . . . . . . . . . . . . . . . . .229
B.9 Suppressor Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .230
B.10 Detector Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .231
B.10.1 Setting the Data Rise Time . . . . . . . . . . . . . . . . . . . . . . . . . . .232
B.10.2 Selecting the Conductivity Polarity . . . . . . . . . . . . . . . . . . . . .232
B.10.3 Setting Analog Out Options . . . . . . . . . . . . . . . . . . . . . . . . . .233
B.11 Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .234
B.12 Module Setup Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235
B.13 Input/Output Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .236
B.14 Diagnostic and Calibration Pages . . . . . . . . . . . . . . . . . . . . . . . . . . . . .236
C • TTL and Relay Control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239
C.1 TTL and Relay Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .239
C.1.1 Selecting TTL Input Functions and Control Types . . . . . . . . .242
C.2 Controlling TTL and Relay Outputs . . . . . . . . . . . . . . . . . . . . . . . . . . .245
C.3 Example Setup for Stand-Alone Operation . . . . . . . . . . . . . . . . . . . . .246
D • Reordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . .251
E•FAQ. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255
E.1 How do I hook up an autosampler? . . . . . . . . . . . . . . . . . . . . . . . . . . .255
E.2 How do I print? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255
E.3 Why are the retention times moving? . . . . . . . . . . . . . . . . . . . . . . . . . .255
viii Doc. 065291-01 3/09
Contents
E.4 How do I adjust retention times? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
E.5 When should I remake standards? . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
E.6 When should I replace the eluent generator cartridge? . . . . . . . . . . . 256
E.7 How do I start Chromeleon? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
E.8 How do I delete data? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
E.9 How do I back up data? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
E.10 How do I shut off the system? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
E.11 How do I store columns? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
E.12 How do I know when a column is dirty? . . . . . . . . . . . . . . . . . . . . . . . 257
E.13 How do I clean a column? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
E.14 Why is the conductivity high? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
E.15 How do I configure and operate the auxiliary valve? . . . . . . . . . . . . . 257
F • Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
Index
Doc. 065291-01 3/09 ix
ICS-2100 Ion Chromatography System
x Doc. 065291-01 3/09
1 • Introduction
1.1 Introduction to Ion Chromatography (IC)
The Dionex ICS-2100 Ion Chromatography System (ICS-2100) performs ion
analyses using suppressed or non-suppressed conductivity detection. An ion
chromatography system typically consists of a liquid eluent, a high-pressure
pump, a sample injector, a guard and separator column, a chemical suppressor, a
conductivity cell, and a data collection system.
Before running a sample, the ion chromatography system is calibrated using a
standard solution. By comparing the data obtained from a sample to that obtained
from the known standard, sample ions can be identified and quantitated. The data
collection system, typically a computer running chromatography software,
produces a chromatogram (a plot of the detector output vs. time). The
chromatography software converts each peak in the chromatogram to a sample
concentration and produces a printout of the results.
Doc. 065291-01 3/09 1
ICS-2100 Ion Chromatography System
n
6
A typical IC analysis consists of six stages (see Figure 1-1).
. Data Analysis
Conductivity
Cell
Suppressor
5. Detectio
1. Eluent
Eluent
Delivery
Pump
2. Sample
Injection
1. Eluent Delivery
• Eluent, a liquid that helps to separate the sample ions, carries the
sample through the ion chromatography system. The ICS-2100
includes an eluent generator, which generates eluent online from
deionized water.
• When the ICS-2100 is controlled from the front panel, only isocratic
eluent delivery is possible. This means that the eluent composition
and concentration remain constant throughout the run. Gradient
delivery (a change in concentration over time) is possible when the
ICS-2100 is controlled by Chromeleon
Management System (the data collection system for the ICS-2100).
4. Suppression
Separator
Column
3. Separation
Guard Column
Injection
Valve
Sample Loop
Sample
Figure 1-1. Ion Analysis Process
®
Chromatography
2. Sample Injection
• The liquid sample is loaded into a sample loop either manually or
automatically (if an automated sampler is installed). When triggered,
the ICS-2100 injects the sample into the eluent stream.
2 Doc. 065291-01 3/09
• The pump pushes the eluent and sample through the guard and
separator columns (chemically-inert tubes packed with a polymeric
resin). The guard column removes contaminants that might poison the
separator column.
3. Separation
• As the eluent and sample are pumped through the separator column,
the sample ions are separated. In the ICS-2100, the mode of
separation is called ion exchange. This is based on the premise that
different sample ions migrate through the IC column at different rates,
depending upon their interactions with the ion exchange sites.
4. Suppression
• After the eluent and sample ions leave the column, they flow through
a suppressor that selectively enhances detection of the sample ions
while suppressing the conductivity of the eluent.
5. Detection
• A conductivity cell measures the electrical conductance of the sample
ions as they emerge from the suppressor and produces a signal based
on a chemical or physical property of the analyte.
1 • Introduction
6. Data Analysis
• The conductivity cell transmits the signal to a data collection system.
• The data collection system (for the ICS-2100, this is the
Chromeleon
ions based on retention time, and quantifies each analyte by
integrating the peak area or peak height. The data is quantitated by
comparing the sample peaks in a chromatogram to those produced
from a standard solution. The results are displayed as a chromatogram
and the concentrations of ionic analytes can be automatically
determined and tabulated.
Doc. 065291-01 3/09 3
®
Chromatography Management System) identifies the
ICS-2100 Ion Chromatography System
1.2 Overview of the ICS-2100
The ICS-2100 is an integrated ion chromatography system containing an eluent
generator, pump, injection valve, column heater, and conductivity detector. Other
system components, including a guard column, separator column, and suppressor
vary, depending on the analyses to be performed.
The ICS-2100 can be configured with a vacuum degas assembly for online eluent
degassing. If desired, the second eluent generator power supply can be configured
to power an auxiliary electrolytic device, such as a water purifier.
An optional second high-pressure valve (6-port or 10-port) can be installed for
sample preparation applications.
ICS-2100 operation can be controlled in one of two ways:
• Remotely, with a personal computer running Microsoft
Windows XP and Chromeleon software (version 6.80 SR6a or later).
Chromeleon also provides data acquisition and data processing functions.
• Locally, with the front panel LCD touch screen. The touch screen is used for
instrument control only. It does not provide data acquisition or data
processing functions. An analog output on the rear panel can be connected to
a separate data acquisition device.
®
Windows®Vista or
For communication between the ICS-2100 and Chromeleon, the ICS-2100 is
connected to a USB (Universal Serial Bus) port on the computer or a USB hub.
For details, see the ICS-2100 installation instructions. Also refer to Installing the
Chromeleon Chromatography Management System with a Dionex Ion
Chromatograph (IC) (Document No. 031883).
4 Doc. 065291-01 3/09
1.3 About This Manual
The electronic version (i.e., PDF file) of this operator’s manual contains numerous
hypertext links that can take you to other locations within the file. These links
include:
• Table of contents entries
• Index entries
• Cross-references (underlined in blue) to sections, figures, tables, etc.
1 • Introduction
If you are not familiar with how to navigate PDF files, refer to the Help system for
Adob
e® Acrobat® or Adobe Reader
Chapter 1
Introduction
Introduces ion analysis and the ICS-2100; explains the
conventions used in this manual, including safety-related
®
for assistance
information.
Chapter 2
Features
Provides an overview of ICS-2100 operating features and
system components; introduces the Chromeleon user
interface.
Chapter 3
Operation and
Maintenance
Chapter 4
Troubleshooting
Chapter 5
Service
Appendix A
Specifications
Appendix B
Touch Screen
Operation
Appendix C
TTL and Relay
Control
Appendix D
Reordering
Information
Provides operating instructions and describes routine
preventive maintenance procedures.
Lists problems, and presents step-by-step procedures for
how to isolate and eliminate the cause of each problem.
Provides step-by-step instructions for routine service and
parts replacement procedures that the user can perform.
Lists the ICS-2100 specifications and installation site
requirements.
Describes the operating features available from the front
panel touch screen.
Describes the ICS-2100 TTL and relay control features.
Lists spare parts for the ICS-2100.
Doc. 065291-01 3/09 5
ICS-2100 Ion Chromatography System
Appendix E
FAQ
Appendix F
Provides answers to frequently asked questions about ICS2100 operation.
Defines terms commonly used in ion analysis.
Glossary
1.3.1 Safety Messages and Notes
This manual contains warnings and precautionary statements that, when
properly followed, can prevent personal injury and/or damage to the
instrument. Safety messages appear in bold type and are accompanied by
icons, as shown below.
Indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury.
Indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
Indicates a potentially hazardous situation which, if not avoided, may
result in minor or moderate injury. Also used to identify a situation or
practice that may seriously damage the instrument, bu t will not c ause
injury.
Indicates that the function or process of the instrument may be
impaired. Operation does not constitute a hazard.
Messages d'avertissement en français
Signale une situation de danger immédiat qui, si elle n'est pas évitée,
entraînera des blessures graves à mortelles.
Signale une situation de danger potentiel qui, si elle n'est pas évitée,
pourrait entraîner des blessures graves à mortelles.
Signale une situation de danger potentiel qui, si elle n'est pas évitée,
pourrait entraîner des blessures mineures à modérées. Également
utilisé pour signaler une situation ou une pratique qui pourrait
gravement endommager l'instrument mais qui n'entraînera pas de
blessures.
6 Doc. 065291-01 3/09
1 • Introduction
Warnhinweise in Deutsch
Bedeutet unmittelbare Gefahr. Mißachtung kann zum Tod oder
schwerwiegenden Verletzungen führen.
Bedeutet eine mögliche Gefährdung. Mißachtung kann zum Tod oder
schwerwiegenden Verletzungen führen.
Bedeutet eine mögliche Gefährdung. Mißachtung kann zu kleineren
oder mittelschweren Verletzungen führen. Wird auch verwendet, wenn
eine Situation zu schweren Schäden am Gerät führen kann, jedoch
keine Verletzungsgefahr besteht.
Notes
Informational messages also appear throughout this manual. These are
labeled NOTE and are in bold type:
NOTE NOTES call attention to certain information. They
alert you to an unexpected result of an action,
suggest how to optimize instrument performance,
etc.
Doc. 065291-01 3/09 7
ICS-2100 Ion Chromatography System
1.4 Safety and Regulatory Information
The ICS-2100 was manufactured by Dionex Corporation at the following
location: 527 Lakeside Drive, Sunnyvale, CA 94088-3603 U.S.A. The ICS-2100
is designed for IC (ion chromatography) applications and should not be used for
any other purpose. If there is a question regarding appropriate usage, contact
Dionex at 1-800-346-6390 before proceeding. Outside the United States, call the
nearest Dionex office.
1.4.1 Safety Labels
The TUV T-Mark and cTUVus Mark safety labels and the CE Mark label
on the system indicate that it is in compliance with the following
standards:
EMC Susceptibility and Emissions
• EN 61326-1:2006
Safety
• CAN/CSA-C22.2 61010-1:2004
• UL 61010-1:2004
• EN 61010-1:2001
The symbols below appear on the ICS-2100 or on labels affixed to the
ICS-2100.
Alternating current
Primary protective conductor terminal
Secondary protective conductor terminal
8 Doc. 065291-01 3/09
1 • Introduction
Power supply is on
Power supply is off
Hot surface
Indicates a potential hazard. Refer to the operator’s manual for
an explanation of the hazard and how to proceed.
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ICS-2100 Ion Chromatography System
10 Doc. 065291-01 3/09
This chapter describes key ICS-2100 features and introduces the Chromeleon user
interface.
2.1 Operating Features
2.1.1 Front Panel
Figure 2-1 illustrates the front panel of the ICS-2100.
Touch
Screen
Power
LED
Injection
Port
2•Features
Injection Port
The sample to be analyzed can
be injected manually into the
injection port, using a syringe.
For automated sample
injection, the ICS-2100 must be
connected to an autosampler.
For more information about
sample injection, see
Section 3.10
Touch Screen
The LCD touch screen provides
local control of most ICS-2100
functions. Yo u can control
many of these functions
directly on the touch screen’s
HOME page (see Figure 2-2).
From the
also go to a series of pages that
provide access to all other
locally controlled ICS-2100
operating functions.
.
HOME page, you can
Figure 2-1. ICS-2100 Front Panel
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ICS-2100 Ion Chromatography System
Summary of Touch Screen Operation
Edit Field
Command
Button
(selected)
Command
Button
(unselected)
Figure 2-2. ICS-2100 Touch Screen Home Page
Status Field
NOTE To adjust the screen contrast, open the front door and
adjust the knurled knob under the screen (see
Figure 2-8
).
• Edit fields and command buttons have blue text on a shaded
background. Fields that display status information are not shaded.
• Edit fields have square corners, while command buttons are rounded.
• To select a command button or edit a field, touch and release the
command button or edit field with your fingertip.
NOTE When you touch a button or edit field, the action is
taken when you lift your finger. If you unintentionally
touch a button or field, you can cancel the action by
sliding your finger away from the button or field before
lifting.
• Touching a command button (for example, ) executes the
command immediately.
12 Doc. 065291-01 3/09
2 • Features
• Selecting a command button changes the button’s appearance to
white text on a dark backgound (for example, ).
• Touching an edit field (for example, ) opens a page
with a number keypad (see Figure 2-3
desired numerical value for the field and then touch the
button.
Touch a
number to
enter the
value.
). Use the keypad to enter the
ENTER
Touch CANCEL
to cancel the
entry and return
to the previous
page.
Touch ENTER to
confirm the
entry and return
to the previous
page.
Figure 2-3. Number Keypad
Doc. 065291-01 3/09 13
ICS-2100 Ion Chromatography System
• Touching the page name (for example, ) in the
bottom right corner opens a menu of pages (see Figure 2-4
Figure 2-4. ICS-2100 Touch Screen Menu of Pages
).
Touch a page
name to display
the page.
• Touching a page name on the menu of p ages displays the selected
page. For example, touching
SUPPRESSOR displays the
14 Doc. 065291-01 3/09
Touch to
return to the
Home page.
2 • Features
SUPPRESSOR page (see Figure 2-5). See Appendix B for details
about each touch screen page.
Touch to
select a
different
option.
Figure 2-5. Suppressor Page
• Touching returns you to the HOME page.
• Touching opens a list of options (see Figure 2-6).
Figure 2-6. Suppressor Page: Selecting an Option
Touch an
option to
select it.
Doc. 065291-01 3/09 15
ICS-2100 Ion Chromatography System
2.1.2 Top Cover
Figure 2-7 illustrates the top cover of the ICS-2100.
EGC, EPM,
CR-TC, and
Auxiliary
Power
Supply
Connectors
Tubing
Chase
(under
connectors)
EGC Service
Area
Figure 2-7. ICS-2100 (Top View)
EluGen
Cartridge
and Holder
Second
EluGen
Cartridge or
Reservoir
Area
Deionized
Water
4 L
Reservoir
Area
EluGen Cartridge and Reservoir
Storage
The top cover holds one or two
®
EluGen
cartridges and up to
two 2-L plastic reservoirs
(P/N 044129) or one 4-L plastic
reservoir (P/N 039164).
EluGen Cartridges
The EluGen cartridges are
installed in holders that fit into
the two back reservoir areas.
See Section 2.4.4
for details
about the eluent generator.
Tubing Chase
The tubing chase under the
EGC and CR-TC connectors
routes tubing from the eluent
reservoir and EGC holder to the
front of the ICS-2100.
EGC, EPM, CR-TC, and Auxiliary Power Supply Connectors
The cable from the EluGen cartridge (EGC) connects to the EGC-1
connector. The cable from the Continuously Regenerated Trap Column
(CR-TC) connects to the
installed, it connects to the
CR-TC connector. If a second EluGen cartridge is
EGC-2 connector. If the second eluent
generator power supply is configured to power an auxiliary electrolytic
device, it connects to the
EGC-2 connector. For details, refer to the ICS-
2100 installation instructions.
When operating with an EGC II K
EluGen Cartridge and the EPM
2CO3
Electrolytic pH Modifier, the EluGen cartridge is typically connected to
the
EGC-1 connector. The pH modifier, which mounts on the side of the
16 Doc. 065291-01 3/09
2 • Features
EluGen cartridge holder, is connected to the EGC-2 connector. However,
the connections can be reversed, if desired.
EGC Service Area
The EGC service area holds the cartridge during installation and
replacement. For details, refer to the ICS-2100 installation instructions.
Doc. 065291-01 3/09 17
ICS-2100 Ion Chromatography System
2.1.3 Component Panel
Figure 2-8 shows the use r-accessible components installed on the
component panel behind the ICS-2100 front door.
12
11
10
8
9
6
7
8
7
6
4
5
5
3
3
Pressure Transducer
1
Leak Sensor
2
Pump Heads
3
Injection Valve
4
Auxiliary Valve
5
(Optional)
DS6 Conductivity Cell
6
Suppressor
7
Column Heater and
8
Columns
Eluent Valve
9
Mounting Brackets for
10
Second Suppressor
Screen Adjustment
11
Knob (under display)
Tubing Chase (2)
12
12
1
1
2
2
Figure 2-8. ICS-2100 Component Panel
18 Doc. 065291-01 3/09
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