ORBECO SP600 Instruction Manual

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SP600 Spectrophotometer

Instruction Manual

Safety precautions

CAUTION

Reagents are formulated exclusively for chemical analysis and must not be used for

any other purpose. Reagents must not get into the hands of children. Some of the

reagents contain substances which are not entirely harmless environmentally. Be

aware of the ingredients and take proper care when disposing of the test solution.

CAUTION

Please read this instruction manual before unpacking, setting up or using the

photometer. Please read the method description completely before performing

the test. Be aware of the risks of using the required reagents by reading the MSDS

(Material Safety Data Sheets). Failure could result in serious injury to the operator or

damage to the instrument.

MSDS:

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SP600 Spectrophotometer_Revision 1 01/2008

SP600 Spectrophotometer 01/2008

Table of contents

Part 1 Methods

1.1 Table of Methods .................................................................................................8

Acid demand to pH 4.3 ....................................................................................................14

Alkalinity-p (p-value) ........................................................................................................16

Alkalinity-total (Alkalinity-m, m-Value) ..............................................................................18

Aluminum with Tablets ....................................................................................................20

Aluminum (Powder Pack) ................................................................................................. 22

Ammonium with Tablets ..................................................................................................24

Ammonium (Powder Pack) ...............................................................................................26

Ammonium LR .................................................................................................................28

Ammonium HR ................................................................................................................30

Arsenic ............................................................................................................................32

Boron ..............................................................................................................................36

Bromine ...............................................................................................................38, 40, 42

Cadmium .........................................................................................................................44

Chloride with Tablets .......................................................................................................46

Chloride ...........................................................................................................................48

Chlorine ...........................................................................................................................50

Chlorine with Tablet

differentiated determination (free, combined, total) .........................................52, 55, 58

free Chlorine ....................................................................................................53, 56, 60

total Chlorine ...................................................................................................54, 57, 61

Chlorine with Liquid Reagent

differentiated determination (free, combined, total) .....................................................62

free Chlorine ................................................................................................................64

total Chlorine ...............................................................................................................65

Chlorine (Powder Pack)

differentiated determination (free, combined, total) .....................................................66

free Chlorine ................................................................................................................68

total Chlorine ...............................................................................................................69

Chlorine HR (Kl) ...............................................................................................................70

Chlorine dioxide, in absence of Chlorine ..........................................................................72

Chlorine dioxide ...............................................................................................................74

in presence of Chlorine ................................................................................................76

in absence of Chlorine .................................................................................................79

Chromium (Powder Pack) .................................................................................................80

differentiated determination ..................................................................................82, 86

Chromium (VI) .......................................................................................................84, 88

total (Cr(III) + Cr(VI)) ............................................................................................... 85, 89

....................................................................................................7

SP600 Spectrophotometer 01/2008

COD LR ............................................................................................................................90

COD MR ..........................................................................................................................92

COD HR ...........................................................................................................................94

Copper with tablet ...........................................................................................................96

differentiated determination (free, combined, total) .............................................98, 102

free Copper .......................................................................................................100, 104

total Copper ......................................................................................................101, 105

Copper PP ......................................................................................................................106

Cyanide .................................................................................................................108, 110

Cyanuric acid ................................................................................................................. 112

DEHA T ..........................................................................................................................114

DEHA PP ........................................................................................................................116

Fluoride .........................................................................................................................118

Formaldehyde ................................................................................................ 120, 122, 124

Hardness, total ...............................................................................................................126

Hardness, total HR .........................................................................................................128

Hazen ............................................................................................................................130

Hydrazine ......................................................................................................................132

Hydrazine with Liquid Reagent .......................................................................................134

Hydrogen peroxide ................................................................................................136, 138

Iodine ............................................................................................................................140

Iron ................................................................................................................................142

Iron with Tablets .........................................................................................144, 146, 148

Iron (Powder Packs) ....................................................................................................150

Iron (TPTZ) (Powder Packs) ..........................................................................................152

Lead ..............................................................................................................................154

Lead, Procedure A ..................................................................................................156, 158

Lead, Procedure B .................................................................................................. 156, 159

Mangenese with Tablet ..................................................................................................160

Manganese LR (Powder Packs) .......................................................................................162

Manganese HR (Powder Packs) ......................................................................................164

Molybdate with Tablets ..................................................................................................166

Molybdate HR (Powder Packs) ........................................................................................168

Nickel ....................................................................................................................170, 172

Nitrate ...........................................................................................................................174

Nitrate LR .......................................................................................................................176

Nitrite with Tablets .........................................................................................................178

Nitrite LR (Powder Packs) ................................................................................................180

Nitrite LR ........................................................................................................................182

Nitrite HR .......................................................................................................................184

SP600 Spectrophotometer 01/2008

Nitrogen, total LR ...........................................................................................................186

Nitrogen, total HR ..........................................................................................................188

Nitrogen, total LR 2 ........................................................................................................190

Nitrogen, total HR 2 .......................................................................................................192

Oxygen, active ...............................................................................................................194

Ozone ............................................................................................................................196

in presence of Chlorine ......................................................................................198, 202

in absence of Chlorine .......................................................................................200, 204

pH-Value with Tablet ...................................................................................................... 206

pH-Value with Liquid Reagent ........................................................................................208

Phenol with Tablets ........................................................................................................210

Phosphate ......................................................................................................................212

Phosphate, total (Tube Test) ........................................................................................214

Phosphate, total LR (Tube Test) ................................................................................... 216

Phosphate, total HR (Tube Test) ..................................................................................218

Phosphate, ortho LR with Tablets ................................................................................220

Phosphate, ortho HR with Tablets ............................................................................... 222

Phosphate, ortho (Powder Packs) ...............................................................................224

Phosphate, ortho (Tube Test) ......................................................................................226

Phosphate, ortho (Tube Test) ......................................................................................228

Phosphate, hydrolysable (Tube Test) ............................................................................230

Potassium ......................................................................................................................232

S Abs, Spectral Absorption Coefﬁcient ........................................................................... 234

Silica ..............................................................................................................................236

Silica LR PP .....................................................................................................................238

Silica HR PP ....................................................................................................................240

Sulfate (Powder Pack) ....................................................................................................242

Sulﬁde ...........................................................................................................................244

Sulﬁte ....................................................................................................................246, 248

Surfactants, anionic .......................................................................................................250

TOC HR .........................................................................................................................252

Turbidity ........................................................................................................................254

Urea ..............................................................................................................................256

Zinc ...............................................................................................................................258

1.2 Important notes ...........................................................................................260

1.2.1 Correct use of reagents ..................................................................................260

1.2.2 Cleaning vials and accessories for analysis ........................................................261

1.2.3 Guidelines for photometric measurements .......................................................262

1.2.4 Sample dilution teqniques ...............................................................................263

1.2.5 Correcting for volume additions ......................................................................263

SP600 Spectrophotometer 01/2008

Part 2 Operating manual ...........................................................................265

2.1 Operating ..................................................................................................... 266

2.1.1 Start Up ..........................................................................................................266

2.1.2 Batteries .......................................................................................................... 266

2.1.3 blank because of technical requirements

2.1.4 Cell chamber and cells .....................................................................................267

2.2 Overview of function keys .........................................................................268

2.2.1 Overview ......................................................................................................... 268

2.2.2 Displaying time and date .................................................................................268

2.2.3 User-countdown ..............................................................................................269

2.3 Operation mode ..........................................................................................270

2.3.1 Self-test ..........................................................................................................270

2.3.2 Selecting a method ..........................................................................................271

2.3.2.1 Method-Information ........................................................................................271

2.3.2.2 Chemical Species Information ..........................................................................271

2.3.3 Differentiation ................................................................................................. 272

2.3.4 Performing Zero ..............................................................................................272

2.3.5 Performing Test ...............................................................................................273

2.3.6 Ensuring reaction periods (countdown) ............................................................273

2.3.7 Changing chemical species .............................................................................. 274

2.3.8 Storing results .................................................................................................274

2.3.9 Printing results .................................................................................................275

2.3.10 Perform additional measurements ...................................................................276

2.3.11 Selecting a new method .................................................................................. 276

2.4 Photometer settings <Mode-Menu>. ......................................................... 277

2.4.1 blank because of technical requirements

2.4.2 Instrument basic settings 1 ..............................................................................278

2.4.3 Printing of stored results ..................................................................................282

2.4.4 Recall / delete stored results .............................................................................287

2.4.5 Calibration ......................................................................................................291

2.4.6 Lab function .................................................................................................... 296

2.4.7 User operations ...............................................................................................304

2.4.8 Special functions .............................................................................................314

2.4.9 Instrument basic settings 2 ..............................................................................316

2.4.10 Instrument special functions /service ................................................................ 317

SP600 Spectrophotometer 01/2008

2.5 Data transfer ................................................................................................318

2.5.1 Connection to a printer ...................................................................................319

2.5.2 Data transfer to a personal computer (PC) .......................................................319

2.5.3 Internet-Updates .............................................................................................320

Part 3 Enclosure ................................................................................................321

3.1 Unpacking ......................................................................................................321

3.2 Delivery content .............................................................................................321

3.3 blank because of technical requirements

3.4 Technical data .................................................................................................322

3.5 Abbreviations .................................................................................................323

3.6 Troubleshooting ...............................................................................................324

3.6.1 Operating messages in the display / error display .............................................324

3.6.2 General problems ............................................................................................ 326

3.6.3 Service / Maintenance ......................................................................................327

3.6.3.1 Handling & Cleaning .......................................................................................327

3.6.3.2 Changing the light source ...............................................................................327

3.6.3.3 blank because of technical requirements

3.6.3.4 Changing the batteries .................................................................................... 329

3.7 Declaration of CE-Conformity ..........................................................................330

SP600 Spectrophotometer 01/2008

Part 1

Methods

SP600 Spectrophotometer 01/2008

Part 1 Methods

1.1 Table of Methods

No. Analysis Reagent Range Displayed asMethod

[nm]

20 Acid demand

tablet 0.1-4 mmol/l Acid /Indicator

1,2, 5

615 14

to pH 4.3 T

35 Alkalinity-p T tablet 5-300 mg/l

CaCO

Alkalinity, total T

tablet 5-200 mg/l

CaCO

Acid/Indicator

40 Aluminum T tablet 0.01-0.3 mg/l Al Eriochrome

Cyanine R

50 Aluminum PP

powder pack

0.01-0.25 mg/l Al Eriochrome Cyanine R

60 Ammonium T tablet 0.02-1 mg/l N

Ammonium PP

Ammonium LR TT

Ammonium HR TT

68 Arsenic

powder pack

0.01-0.8 mg/l N Salicylate

tube test 0.02-2.5 mg/l N Salicylate

tube test 1-50 mg/l N Salicylate

see test

0.02-0.6 mg/l As

instructions

Indophenol blue

Silverdiethyldithiocarbamate

85 Boron T tablet 0.1-2 mg/l B Azomethine

78 Bromine 10 T tablet 0.1-3 mg/l Br

79 Bromine 50 T tablet 0.05-1 mg/l Br

80 Bromine T tablet 0.05-6.5 mg/l Br

87 Cadmium TT tube test

0.025-0.75

mg/l Cd Cadion

90 Chloride T tablet 0.5-25 mg/l Cl

91 Chloride L liquid 5-60 mg/l Cl

Chlorine 10 T *

Chlorine 50 T *

tablet 0.1-6 mg/l Cl2DPD

tablet 0.02-0.5 mg/l Cl2DPD

100 Chlorine T * tablet 0.02-3 mg/l Cl

101 Chlorine L * liquid 0.02-3 mg/l Cl

110 Chlorine PP *

105 Chlorine HR

powder pack

0.01-2 mg/l Cl2DPD

tablet 5-200 mg/l Cl

(Kl) T

119 Chlorine

tablet 0.05-1 mg/l ClO

dioxide 50 T

120 Chlorine

tablet 0.05-2.5 mg/l ClO

dioxide T

DPD

Silver nitrate/turbidity

Iron(III)-thiocyanate

1, 2, 3

DPD

1, 2, 3

DPD

1, 2

KI/Acid

DPD, Glycine

1, 2, 5

551 16

1, 2, 5

615 18

535 20

535 22

2,3

676 24

655 26

655 28

655 30

507 32

450 36

510 38

510 40

510 42

525 44

450 46

455 48

510 50, 52

510 50, 55

510 50, 58

510 50, 62

510 50, 66

1,2

1, 2

470 70

510 72

510 74, 76

Page

* = free, combined, total; PP = powder pack; T = tablet; L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range;

SP600 Spectrophotometer 01/2008

Part 1 Methods

1.1 Table of Methods

No. Analysis Reagent Range Displayed asMethod

[nm]

124 Chromium

powder pack

50 PP

125 Chromium PP

powder pack

130 COD LR TT tube test 0-150 mg/l O

131 COD MR TT tube test 0-1500 mg/l O

132 COD HR TT tube test 0-15 g/l O

149 Copper 50 T tablet 0.05-1 mg/l Cu Biquinoline

150 Copper T * tablet 0.5-5 mg/l Cu Biquinoline

153 Copper PP

powder pack

156 Cyanide 50 L powder +

liquid

157 Cyanide L

powder pack + liquid

Cyanuric acid T

160

tablet 2-160 mg/l Cys Melamine 530 112

165 DEHA T tablet +

0.005-0.5 mg/l Cr 1,5-Diphenylcarbohydrazide

0.02-2 mg/l Cr 1,5-Diphenylcarbohydrazide

Dichromate/H2SO

542 80, 82

1,2

542 80, 86

1,2

1, 2

420 90

1, 2

620 92

1, 2

620 94

559 96, 98

559 96, 102

0.05-5 mg/l Cu Bicinchoninate 560 106

0.005-0.2

0.01-0.5 mg/l CN Pyridine barbituric

20-500 μg/l DEHA PPST

mg/l CN Pyridine barbituric

acid

585 108

585 110

562 114

liquid

167 DEHA PP powder +

20-500 μg/l DEHA PPST

562 116

liquid

170 Fluoride L liquid 0.05-1.5 mg/l F SPADNS

175 Formaldehyde 10powder +

liquid

176 Formaldehyde 50powder +

liquid

1-5 mg/l

HCHO

0.02-1 mg/l

HCHO

177 Formaldehyde TTtube test 0.1-5 mg/l

HCHO

200 Hardness,

total T

201 Hardness, total

HR T

203 Hazen 50

tablet 2-50 mg/l

CaCO

tablet 20-500 mg/l

CaCO

direct

0-500 mg/l Pt-Co Pt-Co-Scale

H2O2/Chromotropic

acid

H2O2/Chromotropic

acid

H2O2/Chromotropic

acid

Metallphthalein3 571 126

Metallphthalein3571 128

580 118

585 120

585 122

575 124

1,2

455 130

reading

205 Hydrazine P powder 0.05-0.5 mg/l N

206 Hydrazine L liquid

0.005-0.6

mg/l N2H44-(Dimethylamino)-

4-(Dimethylamino)-

2H4

benzaldehyde

455 132

455 134

Page

* = free, combined, total; PP = powder pack; T = tablet; L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range;

SP600 Spectrophotometer 01/2008

Part 1 Methods

1.1 Table of Methods

No. Analysis Reagent Range Displayed asMethod

Page

[nm]

209 Hydrogen

peroxide 50 T

210 Hydrogen

peroxide T

tablet 0.01-0.5 mg/l H

tablet 0.03-1.5 mg/l H

2O2

DPD/Catalyst

215 Iodine T tablet 0.05-3.6 mg/l I DPD

218 Iron 10 T tablet 0.1-1 mg/l Fe PPST

219 Iron 50 T tablet 0.01-0.5 mg/l Fe PPST

220 Iron LR T tablet 0.01-1 mg/l Fe PPST

222 Iron PP

223 Iron (TPTZ) PP

powder pack

0.1-3 mg/l Fe

1,10-Phenantroline3510

0.1-1.8 mg/l Fe TPTZ 590

232 Lead 10 liquid 0.1-5 mg/l Pb 4-(2-Pyridylazo)-

resorcin

234 Lead (A) TT tube test 0.1-5 mg/l Pb 4-(2-Pyridylazo)-

resorcin

235 Lead (B) TT tube test 0.1-5 mg/l Pb 4-(2-Pyridylazo)-

resorcin

510 136

510 138

510 140

562

142, 144

562

142, 146

562

142, 148

142, 150

142, 152

520 154

515

156, 158

515

156, 159

240 Manganese T tablet 0.2-4 mg/l Mn Formaldoxime 450 160

242 Manganese

LR PP

243 Manganese

HR PP

250 Molybdate T tablet 1-30 mg/l

252 Molybdate

HR PP

255 Nickel 50 L powder +

256 Nickel L powder +

265 Nitrate TT tube test 1-30 mg/l N

267 Nitrate LR TT tube test 0.5-14 mg/l N 2,6-Dimethyl-

270 Nitrite T tablet 0.01-0.5 mg/l N N-(1-Naphthyl)-

272 Nitrite PP

powder pack + liquid

powder pack

liquid

powder pack

0.01-0.7 mg/l Mn PAN 558 162

0.1-18 mg/l Mn Periodate oxidation

Thioglycolate

MoO

0.5-66 mg/l

MoO

Mercaptoacetic acid

0.02-1 mg/l Ni Dimethylglyoxime

0.2-7 mg/l Ni Dimethylglyoxime

Chromotropic acid

2,3

phenol

ethylendiamine

2,3

525 164

366 166

420 168

443 170

443 172

410 174

340 176

545 178

2,3

0.01-0.3 mg/l N Diazotization 507 180

* = free, combined, total; PP = powder pack; T = tablet; L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range;

SP600 Spectrophotometer 01/2008

Part 1 Methods

1.1 Table of Methods

No. Analysis Reagent Range Displayed asMethod

275 Nitrite LR TT tube test 0.03-0.6 mg/l N Sulfanilic acid/

Naphthylamine

276 Nitrite HR TT tube test 0.3-3 mg/l N Sulfanilic acid/

Naphthylamine

total

280 Nitrogen,

LR TT

281 Nitrogen,

HR TT

283 Nitrogen, total

LR 2 TT

284 Nitrogen, total

HR 2 TT Oxygen, active T

290 299 Ozone

(DPD) 50

300 Ozone

(DPD) T

tube test 0.5-25 mg/l N Persulfate

digestion method

total

tube test 5-150 mg/l N Persulfate

digestion method

tube test 0.5-14 mg/l N 2,6 Dimethyl-

phenol

tube test 5-140 mg/l N 2,6 Dimethyl-

phenol tablet 0.1-10 mg/l O2DPD 510 194 tablet 0.02-0.5 mg/l O

tablet 0.02-1 mg/l O

DPD/Glycine

330 pH-Value T tablet 6.5-8.4 — Phenol red 331 pH-Value L liquid 6.5-8.4 — Phenol red 315 Phenol T tablet 0.1-5 mg/l

326 Phosphate,

tube test 0.02-1.1 mg/l P

6H5

total TT

317 Phosphate,

tube test 0.07-3 mg/l P

total LR TT

4-Aminoantipyrine 1507 210

Acid persulf. digestion,

Ascorbic acid

Phosphomolybdic

acid /

Ascorbic acid

318 Phosphate,

total HR TT

tube test 1.5-20 mg/l P

Phosphomolybdic

acid/

Ascorbic acid

320 Phosphate,

ortho LR T

321 Phosphate,

ortho HR T

323 Phosphate,

tablet 0.05-4 mg/l PO

tablet 1-80 mg/l PO

powder pack

0.06-2.5 mg/l PO4Ascorbic acid

Ammonium-

molybdate

Vanando

molybdate

2,3

2, 3

ortho PP

324 Phosphate,

tube test 0.06-5 mg/l PO4Ascorbic acid

ortho TT

322 Phosphate,

ortho (VM) TT

tube test 3-60 mg/l PO

Vanadomolybdate 2438 212,

Page

[nm]

545 182

545 184

410 186

410 188

340 190

340 192

510 196,

198

510 196,

202 558 206 558 208

890 212,

214 690 212,

216

690 212,

218

710 212,

220 470 212,

222 890 212,

224 890 212,

226

228

* = free, combined, total; PP = powder pack; T = tablet; L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range;

SP600 Spectrophotometer 01/2008

Part 1 Methods

1.1 Table of Methods

No. Analysis Reagent Range Displayed asMethod

[nm]

325 Phosphate,

hydr. TT

340 Potassium T tablet 1-10 mg/l K

345 S Abs 436 nm

(Colour)

346 S Abs 525 nm

(Colour)

347 S Abs 620 nm

(Colour)

350 Silica T tablet 0.05-3 mg/l SiO

351 Silica LR PP

352 Silica HR PP

360 Sulfate PP

365 Sulﬁde tablet 0.04-0.5 mg/l S

368 Sulﬁte 10 T tablet 0.1-10 mg/l SO

370 Sulﬁte T tablet 0.05-4 mg/l SO

375

Surfactants

tube test 0.02-1.6 mg/l P

direct

0-50 m-1 EN ISO

reading

direct

0-50 m-1 EN ISO

reading

direct

0-50 m-1 EN ISO

reading

powder pack

tube test 0.05-2

0.1-1.6 mg/l SiO2Heteropolyblue 2815 238

1-100 mg/l SiO2Silicomolybdate 452 240

2-100 mg/l SO4Bariumsulfate-

mg/l MBAS Methylene blue

Acid digestion/ Ascorbic acid

TetraphenylborateTurbidity

7887:1994

Silicomolybdate

Turbidity

–

DPD/Catalyst

DTNB 405 246

DTNB 405 248

2, 3

3,4

6,1

890 212,

730 232

436 234

525 234

620 234

820 236

450 242

668 244

653 250

(anionic) TT

381 TOC HR TT tube test 50-800 mg/l TOC

385 Turbidity 50 direct

5-500 FAU Attenuated

reading

390 Urea T tablet +

0.1-2 mg/l Urea Indophenol/

liquid

400 Zinc T tablet 0.02-0.5 mg/l Zn Zincon

H2SO4 /Persulfate /

Indicator

Radiation Method

Urease

596 252

860 254

676 256

616 258

Page

230

* = free, combined, total; PP = powder pack; T = tablet; L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range;

SP600 Spectrophotometer 01/2008

1.1 Methods

Literature

The reagent formulations are based on internationally recognised test methods. Some are described in national and/or international guidelines.

1) Deu tsche Einh eitsverfahren zur Wasser-, Abw asser- und Sc hlammuntersuchung

2) Standard methods for the Examination of Water and Wastewater; 18th Edition, 1992

3) Photometrische Analysenverfahren, Schwedt, Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart 1989

4) Photometrische Analyse, Lange / Vejdelek, Verlag Chemie 1980

5) Colorimetric Chemical Analytical methods, 9th Edition, London

6) adapted from Merck, for more information see instructions delivered with the test

Notes for searching:

Active Oxygen -> Oxygen, activ Alkalinity-m -> Alkalinity, total Alkalinity, total -> Alkalinity, total Colour -> Hazen or Spectral Absorption Coefﬁcient (S Abs) Total Hardness -> Hardness, total m-Value -> Alkalinity, total p-Value -> Alkalinity-p Phosphate, reactive -> Phosphate, ortho Silicon dioxide -> Silica

Langelier Saturation -> Mode function 70 Index (Water Balance)

SP600 Spectrophotometer 01/2008

1.1 Methods

Acid demand to pH 4.3

with Tablet

0.1 – 4 mmol/l

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

2. Place the vial in the sample chamber making sure that the

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one ALKA-M-PHOTOMETER tablet straight from the foil to the water sample and crush the tablet using a clean stirring rod.

6. Close the vial tightly with the cap and swirl several times until the tablet is dissolved.

7. Place the vial in the sample chamber making sure that the

8. Press TEST key.

The result is shown in the display as Acid demand to pH 4.3 in mmol/l.

marks are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. The terms total Alkalinity, Alkalinity-m, m-Value and Acid demand to pH 4.3 are identical.

2. For accurate results exactly 10 ml of water sample must be taken for the test.

SP600 Spectrophotometer 01/2008

1.1 Methods

Alkalinity-p = p-value

with Tablet

5 – 300 mg/l CaCO

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that marks are aligned.

the

prepare Zero press ZERO

Zero accepted prepare Test press TEST

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one ALKA-P-PHOTOMETER tablet straight

from the foil to the water sample and crush the tablet using a clean stirring rod.

6. Close the vial tightly with the cap and swirl several

times until the tablet is dissolved.

7. Place the vial in the sample chamber making sure that marks are aligned.

the

8. Press TEST key.

The result is shown in the display as Alkalinity-p.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes

1. The terms Alkalinity-p, p-Value and Alkalinity to pH 8.2 are identical.

2. For accurate test results exactly 10 ml of water sample must be taken for the test.

3. This method was developed from a volumetric procedure for the determination of Alkalinity-p. Due to undeﬁned conditions, the deviations from the standardised method may be greater.

4. Conversion table:

1 mg/l CaCO

mg/l CaCO

---- 0.056 0.10 0.07

1 °dH 17.8 ---- 1.78 1.25

1 °fH 10.0 0.56 ---- 0.70

1 °eH 14.3 0.80 1.43 ----

CaCO

°dH °eH °fH °aH

5. By determining Alkalinity-p and Alkalinity-m it is possible to classify the alkalinity as

Hydroxide, Carbonate and Hydrogencarbonate. The following differentiation is only valid if: a) no other alkalis are present and b) Hydroxide und Hydrogen are not present in the same water sample. If condition b) is not fulﬁlled please get additional information from

”Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung, D 8”. Case 1: Alkalinity-p = 0

Hydrogen carbonate = m Carbonate = 0 Hydroxide = 0

Case 2: Alkalinity-p > 0 and Alkalinity-m > 2p Hydrogen carbonate = m – 2p Carbonate = 2p Hydroxide = 0

Case 3: Alkalinity-p > 0 and Alkalinity-m < 2p Hydrogen carbonate = 0 Carbonate = 2m – 2p Hydroxide = 2p – m

°dH °fH °eH

SP600 Spectrophotometer 01/2008

1.1 Methods

Alkalinity, total = Alkalinity-m =

m-Value with Tablet

5 – 200 mg/l CaCO

Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

2. Place the vial in the sample chamber making sure that the

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one ALKA-M-PHOTOMETER tablet straight from the foil to the water sample and crush the tablet using a clean stirring rod.

6. Close the vial tightly with the cap and swirl several times until the tablet is dissolved.

7. Place the vial in the sample chamber making sure that the

8. Press TEST key.

The result is shown in the display as total Alkalinity.

marks are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. The terms total Alkalinity, Alkalinity-m, m-Value and Alkalinity to pH 4.3 are

identical.

2. For accurate results exactly 10 ml of water sample must be taken for the test.

3. Conversion table:

1 mg/l CaCO

Acid demand to pH 4.3

DIN 38 409 (KS4.3)

0.02 0.056 0.07 0.1

German

°dH*

*Carbonate hardness (reference = Hydrogencarbonate-anions)

Example: 10 mg/l CaCO

= 10 mg/l x 0.056 = 0.56 mg/l °dH

10 mg/l CaCO3 = 10 mg/l x 0.02 = 0.2 mmol/l

CaCO

°dH °eH °fH °aH

English

°eH*

French

°fH*

SP600 Spectrophotometer 01/2008

1.1 Methods

Aluminum

with Tablet

0.01 – 0.3 mg/l Al

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that marks are aligned.

the

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 5:00

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one ALUMINIUM No. 1 tablet straight from the

foil to the water sample and crush the tablet using a clean stirring rod (dissolve the tablet).

6. Add one ALUMINIUM No. 2 tablet straight from the

foil to the same water sample and crush the tablet using a clean stirring rod.

7. Close the vial tightly with the cap and swirl gently

several times until the tablets are dissolved.

8. Place the vial in the sample chamber making sure that marks are aligned.

the

9. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in mg/l Aluminium.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Before using clean the vials and the measuring beaker with Hydrochloric acid (approx. 20%). Rinse then thoroughly with deionized water.

2. To get accurate results the sample temperature must be between 20°C and 25°C.

3. A low test result may be given in the presence of Fluorides and Polyphosphates. The effect of this is generally insigniﬁcant unless the water has ﬂuoride added artiﬁcially. In this case, the following table should be used:

Fluoride [mg/l F]

Displayed value: Aluminium [mg/l Al]

0.05 0.10 0.15 0.20 0.25 0.30

0.2 0.05 0.11 0.16 0.21 0.27 0.32

0.4 0.06 0.11 0.17 0.23 0.28 0.34

0.6 0.06 0.12 0.18 0.24 0.30 0.37

0.8 0.06 0.13 0.20 0.26 0.32 0.40

1.0 0.07 0.13 0.21 0.28 0.36 0.45

1.5 0.09 0.20 0.29 0.37 0.48 ---

Example: If the result of Aluminium determination is 0.15 mg/l Al and the Fluoride concentration is known to be 0.4 mg/l F, the true concentration of Aluminium is

0.17 mg/l Al.

Al2O

SP600 Spectrophotometer 01/2008

1.1 Methods

1 2

3 4

Aluminum

with Powder Pack

0.01 – 0.25 mg/l Al

Use two clean vials (24 mm Ø) and mark one as blank for zeroing.

1. Fill 20 ml of water sample in a 100 ml beaker.

2. Add one Vario Aluminum ECR F20 powder pack straight from the foil to the water sample.

3. Dissolve the powder using a clean stirring rod.

Countdown 1 0:30 start:

Countdown 2 5:00 start:

4. Press [ Wait for a reaction period of 30 seconds.

After reaction period is ﬁnished proceed as follows:

5. Add one Vario Hexamine F20 powder pack straight from the foil to the same water sample.

6. Dissolve the powder using a clean stirring rod.

7. Add 1 drop of Vario Aluminum ECR Masking Re- agent in the vial marked as blank.

8. Add 10 ml of the prepared water sample to the vial (this is the blank).

9. Add the remaining 10 ml of the prepared water sample in the second clean vial (this is the sample).

10. Close the vials tightly and swirl several times to mix the contents.

11. Press [ Wait for a reaction period of 5 minutes.

] key.

SP600 Spectrophotometer 01/2008

1.1 Methods

After reaction period is ﬁnished proceed as follows:

12. Place the vial (the blank) in the sample chamber marks are aligned.

prepare Zero press ZERO

making sure that the

13. Press ZERO key.

14. Remove the vial from the sample chamber.

15. Place the vial (the sample) in the sample chamber

making sure that the

marks are aligned.

Zero accepted prepare Test

16. Press TEST key.

press TEST

The result is shown in the display in mg/l Aluminium.

Notes:

1. Before using clean the vials and the measuring beaker with Hydrochloric acid (approx. 20%). Rinse then thoroughly with deionized water.

2. To get accurate results the sample temperature must be between 20°C and 25°C.

Fluoride [mg/l F]

Displayed value: Aluminium [mg/l Al]

0.05 0.10 0.15 0.20 0.25 0.30

0.2 0.05 0.11 0.16 0.21 0.27 0.32

0.4 0.06 0.11 0.17 0.23 0.28 0.34

0.6 0.06 0.12 0.18 0.24 0.30 0.37

0.8 0.06 0.13 0.20 0.26 0.32 0.40

1.0 0.07 0.13 0.21 0.28 0.36 0.45

1.5 0.09 0.20 0.29 0.37 0.48 ---

Example: If the result of Aluminium determination is 0.15 mg/l Al and the Fluoride concentration is known to be 0.4 mg/l F, the true concentration of Aluminium is

0.17 mg/l Al.

Al2O

SP600 Spectrophotometer 01/2008

1.1 Methods

Ammonium

with Tablet

0.02 – 1 mg/l N

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that marks are aligned.

the

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 10:00

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one AMMONIA No. 1 tablet straight from the

foil to the water sample and crush the tablet using a clean stirring rod.

6. Add one AMMONIA No. 2 tablet straight from the

foil to the same water sample and crush the tablet using a clean stirring rod.

7. Close the vial tightly with the cap and swirl several

times until the tablets are dissolved.

8. Place the vial in the sample chamber making sure that marks are aligned.

the

9. Press TEST key.

Wait for a reaction period of 10 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in mg/l Ammonium.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. The tablets must be added in the correct sequence.

2. The AMMONIA No. 1 tablet will only dissolve completely after the AMMONIA No. 2 tablet has been added.

3. The temperature of the sample is important for full colour development. At a temperature below 20°C the reaction period is 15 minutes.

4. Sea water samples: Ammonia conditioning reagent is required when testing sea water or brackish water samples to prevent precipitations of salts.

Fill the test tube with the sample to the 10 ml mark and add one level spoonful of Conditioning Powder. Mix to dissolve, then continue as described in the test instructions.

5. Conversion: mg/l NH4 = mg/l N x 1.29 mg/l NH3 = mg/l N x 1.22

6. N NH NH

SP600 Spectrophotometer 01/2008

1.1 Methods

1 2

3 4

Countdown 3:00 start:

Ammonium

with Powder Pack

0.01 – 0.8 mg/l N

Use two clean vials (24 mm Ø) and mark one as blank for zeroing.

1. Fill a clean vial (24 mm Ø) with 10 ml of deionized

water (this is the blank).

2. Fill the other clean vial (24 mm Ø) with 10 ml of the

water sample (this is the sample).

3. Add one Vario Ammonium Salicylate F10 powder

pack straight from the foil to each vial.

4. Close the vials with the caps and shake to mix the con-

tents.

5. Press [

Wait for a reaction period of 3 minutes.

After reaction period is ﬁnished proceed as follows:

6. Add one Vario Ammonium Cyanurate F10 powder

] key.

pack straight from the foil to each sample.

Countdown 15:00 start:

prepare Zero press ZERO

Zero accepted prepare Test press TEST

7. Close the vials with the caps tightly and shake to mix

the contents.

8. Press [ ] key.

Wait for a reaction period of 15 minutes.

After reaction period is ﬁnished proceed as follows:

9. Place the vial (the blank) in the sample chamber making

sure that the marks are aligned.

10. Press ZERO key.

11. Remove the vial from the sample chamber.

12. Place the vial (the sample) in the sample chamber

making sure that the marks are aligned.

13. Press TEST key.

The result is shown in the display in mg/l Ammonium.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Extremely basic or acidic water samples should be adjusted with 0.5 mol/l (1 N) Sulfuric acid solution or 1 mol/l (1 N) Sodium hydroxide solution to pH 7.

2. Interferences:

Interfering substance Interference levels and treatments

Calcium greater than 1000 mg/l CaCO

Iron Interferes at all levels. Correct as follows:

a) determine the concentration of iron present in the sample by performing a total Iron test

b) add the same iron concentration as determined to the deionized water (step 1).

The interference will be blanked out successfully.

Magnesium greater than 6000 mg/l CaCO

Nitrate greater than 100 mg/l NO3-N

Nitrite greater than 12 mg/l NO2-N

Phosphate greater than 100 mg/l PO4-P

Sulfate greater than 300 mg/l SO

Sulﬁde intensiﬁes the colour

Glycine, Hydrazine, Colour, Turbidity

Less common interferences such as Hydrazine and Glycine will cause intensiﬁed colours in the prepared sample. Turbidity and colour will give erroneous high values. Samples with severe interferences require distillation.

N NH NH

SP600 Spectrophotometer 01/2008

1.1 Methods

1 2

3 4

Ø 16 mm

Ammonium LR

with Tube Test

0.02 – 2.5 mg/l N

1. Open one white capped reaction vial and add 2 ml deionized water (this is the blank).

2. Open another white capped reaction vial and add 2 ml of water sample (this is the sample).

3. Add one Vario AMMONIA Salicylate F5 powder pack straight from the foil into each vial.

4. Add one Vario AMMONIA Cyanurate F5 powder pack straight from the foil into each vial.

5. Close the vials tightly and swirl several times to dissolve the powder.

Countdown 20:00 start:

prepare Zero press ZERO

Zero accepted prepare Test press TEST

6. Press [ Wait for a reaction period of 20 minutes. After reaction period is ﬁnished proceed as follows:

7. Place the vial (the blank) in the sample chamber making sure that the marks are

8. Press ZERO key.

9. Remove the vial from the sample chamber.

10. Place the vial (the sample) in the sample chamber making sure that the marks are

11. Press TEST key.

The result is shown in the display in mg/l Ammonium.

] key.

aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Strong alkaline or acidic water samples must be adjusted to approx. pH 7 before analysis (use 1 mol/l Hydrochloric acid resp. 1 mol/l Sodium hydroxide).

2. If chlorine is known to be present, add one drop of 0.1 mol/l Sodium thiosulfate for each 0.3 mg/l Cl2 in a one litre water sample.

3. Iron interferes with the test. The interferences will be eliminated as follows: Determine the amount of total iron present in the water sample. To produce the blank add an iron standard solution with the same iron concentration to the vial (point 1) instead of deionized water

4. Conversion: mg/l NH4 = mg/l N x 1.29 mg/l NH3 = mg/l N x 1.22

5. N NH NH

SP600 Spectrophotometer 01/2008

1.1 Methods

1 2

3 4

Ø 16 mm

Ammonium HR

with Tube Test

1 – 50 mg/l N

1. Open one white capped reaction vial and add 0.1 ml deionized water (this is the blank).

2. Open another white capped reaction vial and add

0.1 ml of water sample (this is the sample).

3. Add one Vario AMMONIA Salicylate F5 powder pack straight from the foil into each vial.

4. Add one Vario AMMONIA Cyanurate F5 powder pack straight from the foil into each vial.

5. Close the vials tightly and swirl several times to dissolve the powder.

Countdown 20:00 start:

prepare Zero press ZERO

Zero accepted prepare Test press TEST

6. Press [ Wait for a reaction period of 20 minutes. After reaction period is ﬁnished proceed as follows:

7. Place the vial (the blank) in the sample chamber making sure that the marks are

8. Press ZERO key.

9. Remove the vial from the sample chamber.

10. Place the vial (the sample) in the sample chamber making sure that the marks are

11. Press TEST key.

] key.

aligned.

The result is shown in the display in mg/l Ammonium.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Strong alkaline or acidic water samples must be adjusted to approx. pH 7 before analysis (use 1 mol/l Hydrochloric acid resp. 1 mol/l Sodium hydroxide).

2. If chlorine is known to be present, add one drop of 0.1 mol/l Sodium thiosulfate for each

0.3 mg/l Cl2 in a one litre water sample.

3. Iron interferes with the test. The interferences will be eliminated as follows: Determine the amount of total iron present in the water sample. Add an iron standard solution with the same concentration to the vial (point 1) instead of deionized water to produce the blank.

4. Conversion: mg/l NH4 = mg/l N x 1.29 mg/l NH3 = mg/l N x 1.22

5. N NH NH

SP600 Spectrophotometer 01/2008

1.1 Methods

Arsen

0.02 – 0.6 mg/l As

Reagents (note 2):

• 40%SulfuricAcid(H2SO4) p.a.

• Dissolve8.33gPotassiumIodide(KI)p.a.in50mlof

deionized water Note: stored in a dark bottle it can be used for 1 week

•

Dissolve 4.0 g Tin(II)-chloride-Dihydrate (SnCl2•2H2O) p.a. in 10 ml Hydrochloric Acid (HCl) 25 % p.a.

• 2.0gZinccoarsepowder (Zn; particle size about 0.3-1.5 mm) p.a.

• Absorptionsolution:

Dissolve 0.25 g Silver diethyldithiocarbamate (C

AgNS2) p.a.

5H10

and

0.02 g Brucine (C23H26N2O4) p.a. in 100 ml 1-Methyl-2-pyrrolidone extra pure (C and store in a dark bottle. If it is not possible to dissolve completely, stir for min. 1 hour and ﬁltrate to get a clear solution.

5H9

NO)

Notes:

• useonlydryglassvessels

• storedinadarkglassbottleatmax.20°Ctheabsorp-

tion solution can be used for about 1 week

• storeSilverdiethyldithiocarbamateat4°C.

Part list for glass apparatus:

•100mlErlenmeyerask(NS29/32) Ordercode:370501

•glassstopper(NS29/32) Ordercode:370502

•absorptiontube(NS29,2/32) Ordercode:370503

Assembling of apparatus:

SP600 Spectrophotometer 01/2008

1.1 Methods

Sample preparation: Reaction times must be exactly kept !

1. Prepare the dry reaction apparatus (note 4) and place it under a fume hood (toxic fumes!).

2. Pipette 50 ml of water sample into a 100 ml Erlenmeyer ﬂask (NS 29/32).

3. 30 ml Sulfuric Acid,

2.0 ml Potassium Iodide solution and

0.3 ml Tin(II)chloride are added to the water sample.

4. Close the flask and shake, wait for a period of 15 minutes.

5. Prepare 2.0 g Zinc.

6. Fill the absorption tube with exact 5.0 ml of absorption solution

(see picture

7. After end of the 15 minutes reaction time add the 2 g Zinc to the Erlenmeyer ﬂask and immediately as- semble the apparatus with the prepared absorption

tube (see picture

8. The reaction starts (fume hood!). Wait for 60 minutes reaction time.

and ; use pipette).

Performing test procedure:

prepare Zero press ZERO

Zero accepted prepare Test press TEST

11. Press ZERO key.

12. After zeroing remove the cell from the sample chamber.

13. Fill the cell with the colored absorption solution. (see

14. Place the cell in the sample chamber making sure that

15. Press TEST key.

The result is shown in the display in mg/l Arsenic

Fill a clean 20 mm cell (note 1) with deionized water.

Place the cell in the sample chamber making sure that the positioning is correct.

Empty the cell and dry completely.

picture

the marks are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Appropriate safety precautions and a good lab technique must be used during the whole procedure.

2. Reagents are commercially and should ordered locally. MSDS: please refer to your local reagent supplier. Ensure proper disposal of reagent

solution.

3. Use a cell with 20 mm path length. Order code: 60 10 50. Positioning: insert cell on the left side in the sample chamber (c = clip).

4. According to literature (G. Ackermann, J. Köthe: Fresenius Z. Anal. Chem. 323 (1986), 135) Sb, Se and Te interfere due to the same reaction; Thiosulfate interferes differently.

SP600 Spectrophotometer 01/2008

1.1 Methods

Boron

with Tablet

0.1 – 2 mg/l B

Fill a clean vial (24 mm Ø) with 10 ml of the water

mple, close thightly with the cap.

2. Place the vial in the sample chamber making sure that marks are aligned.

the

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 20:00

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one BORON No. 1 tablet straight from the foil

to the water sample and crush the tablet using a clean stirring rod and dissolve the tablet.

6. Add one BORON No. 2 tablet straight from the foil

to the same water sample and crush the tablet using a clean stirring rod.

7. Close the vial tightly with the cap and swirl several

times until the tablets are dissolved.

8. Place the vial in the sample chamber making sure that marks are aligned.

the

9. Press TEST key.

Wait for a reaction period of 20 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display in mg/l Boron.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. The tablets must added in the correct sequence.

2. The sample solution should have a pH value between 6 and 7.

3. Interferences are prevented by the presence of EDTA in the tablets.

4. The rate of colour development depends on the temperature. The temperature of the sample must be 20°C ± 1°C.

5. B H3BO

SP600 Spectrophotometer 01/2008

1.1 Methods

Bromine

with Tablet

0.1 – 3 mg/l Br

1. Fill a clean 10 mm cell with water sample.

2. Place the cell in the sample chamber making sure that

the positioning is correct.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the

cell and dry completely.

5. Rinse a beaker with the water sample and empty it,

leaving a few drops remaining in the beaker.

6. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

8. Fill the 10 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that

the positioning is correct.

10. Press TEST key.

The result is shown in the display in mg/l Bromine.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Vial cleaning: As many household cleaners (e.g. dishwater detergent) contain reducing substances, the subsequent determination of Bromine may show lower results. To avoid any measurement errors, only use glassware free of Chlorine consumption. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionized water.

2. Preparing the sample: When preparing the sample, the escape of Bromine gases, e.g. by pipetting or shaking, must be avoided. The analysis must take place immediately after taking the sample.

3. The DPD colour development is carried out at a pH value of 6.3 to 6.5. The reagent tablet therefore contains a buffer for pH adjustment. Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the reagent is added (use 0.5 mol/l Sulfuric acid resp. 1 mol/l Sodium hydroxide).

4. Exceeding the measuring range: Concentrations above 22 mg/l Bromine can produce results within the measuring range up to 0 mg/l. In this event, the water must be diluted with water free of Bromine. 10 ml of the diluted sample will be mixed with the reagent and the measurement repeated.

Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Bromine.

SP600 Spectrophotometer 01/2008

1.1 Methods

Bromine

with Tablet

0.05 – 1 mg/l Br

1. Fill a clean 50 mm cell with water sample.

2. Place the cell in the sample chamber making sure that

the positioning is correct.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the

cell and dry completely.

5. Rinse a beaker with the water sample and empty it,

leaving a few drops remaining in the beaker.

6. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

8. Fill the 50 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that

the positioning is correct.

10. Press TEST key.

The result is shown in the display in mg/l Bromine.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

2. Preparing the sample: When preparing the sample, the escape of Bromine gases, e.g. by pipetting or shaking, must be avoided. The analysis must take place immediately after taking the sample.

Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Bromine.

SP600 Spectrophotometer 01/2008

1.1 Methods

Bromine

with Tablet

0.05 – 6.5 mg/l Br2

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,

close tightly with the cap.

2. Place the vial in the sample chamber making sure that

marks are aligned.

the

prepare Zero press ZERO

Zero accepted prepare Test press TEST

3. Press ZERO key.

4. Remove the vial from the sample chamber, empty the

vial leaving a few drops in.

5. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

6. Add water sample to the 10 ml mark.

7. Close the vial tightly with the cap and swirl several

times until the tablet is dissolved.

8. Place the vial in the sample chamber making sure that

marks are aligned.

the

9. Press TEST key.

The result is shown in the display in mg/l Bromine.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing substances, the subsequent determination of Bromine may show lower results. To avoid any measurement errors, only use glassware free of Chlorine consumption. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionized water.

2. Preparing the sample: When preparing the sample, the escape of Bromine gases, e.g. by pipetting or shaking, must be avoided. The analysis must take place immediately after taking the sample.

3. The DPD colour development is carried out at a pH value of 6.3 to 6.5. The reagent tablet therefore contains a buffer for the pH adjustment. Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the reagent is added (use 0.5 mol/l Sulfuric acid resp. 1 mol/l Sodium hydroxide).

4. Exceeding of the measuring range: Concentrations above 22 mg/l Bromine can produce results within the measuring range up to 0 mg/l. In this event, the water sample must be diluted with water free of Bromine. 10 ml of the diluted sample will be mixed with the reagent and the measurement repeated.

Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Bromine.

SP600 Spectrophotometer 01/2008

1.1 Methods

Cadmium

with MERCK Spectroquant

Cell Test,

Nr. 1.14834.0001

0.025 – 0.75 mg/l Cd / 25 – 750 μg/l Cd

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 2:00

Ø 16 mm

1. Place the supplied blank in the sample chamber making sure that the marks are

2. Press ZERO key.

3. Remove the vial from the sample chamber.

4. Add 5 ml of water sample into one reaction tube.

5. Close the vial tightly with the cap and invert several times to mix the contents.

6. Add 0.2 ml reagent Cd-1K.

7. Close the vial tightly with the cap and invert several times to mix the contents.

8. Add one level microspoon of reagent Cd-2K.

9. Close the vial tightly with the cap and swirl until the reagent is solved completely.

10. Place the vial in the sample chamber making sure that the marks are

11. Press TEST key.

Wait for a reaction period of 2 minutes.

aligned.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display as Cadmium.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. This method is adapted from MERCK.

2. Before performing the test read the original test instructions (delivered with the test) and the MSDS (available at www.merck.com).

3. Spectroquant

4. Appropriate safety precautions and good lab technique should be used during the whole procedure.

5. Because reaction depends on temperature, the sample temperature must be between 10 and 40°C.

6. Sample and reagent volumes should always metered by using volumetric pipettes (class A).

7. This test determines only Cd by digestion before colloidal, undissolved and complex-bounded cadmium can be measured.

mg/l μg/l

is a registered trade mark of the company MERCK KGaA.

-ions. Samples must be pre-treated or decomposed

SP600 Spectrophotometer 01/2008

1.1 Methods

Chloride

with Tablet

0.5 – 25 mg/l Cl

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that the

–

marks are aligned.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 2:00

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one CHLORIDE T1 tablet straight from the foil to the water sample, crush the tablet using a clean stirring rod and dissolve the tablet.

6. Add one CHLORIDE T2 tablet straight from the foil to the same water sample and crush the tablet using a clean stirring rod.

7. Close the vial tightly with the cap and swirl gently several times until the tablet is dissolved (Note 1).

8. Place the vial in the sample chamber making sure that marks are aligned.

the

9. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in mg/l Chloride.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Ensure that all particles of the tablet are dissolved – Chloride causes an extremely ﬁne distributed turbidity with a milky appearance. Heavy shaking leads to bigger sized particles which can cause false readings.

2. High concentrations of electrolytes and organic compounds have different effects on the precipitation reaction.

3. Ions which also form deposits with Silver nitrate in acidic media, such as Bromides, Iodides and Thiocyanates, interfere with the analysis.

4. Highly alkaline water should – if necessary – be neutralised using Nitric acid before analysis.

SP600 Spectrophotometer 01/2008

1.1 Methods

Chloride

with Reagent Test

5 – 60 mg/l Cl

Use two clean vials (24 mm Ø) and mark one as blank for zeroing.

1. Fill a clean vial (24 mm Ø) with 10 ml of deionized

water (this is the blank).

2. Fill the second clean vial (24 mm Ø) with 1 ml of the

water sample and 9 ml of deionized water (this is the sample).

3. Fill each vial with the same size drops by holding the

bottle vertically and squeeze slowly:

3 drops Chloride-51

4. Close the vials with the caps tightly and invert the vials

several times to mix the contents.

5. Fill each vial with the same size drops by holding the

bottle vertically and squeeze slowly:

–

Countdown 3:00 start:

prepare Zero press ZERO

Zero accepted prepare Test press TEST

3 drops Chlorid-52

6. Close the vials with the caps tightly and invert the vials

several times to mix the contents.

7. Press [

Wait for a reaction period of 3 minutes.

8. Place the vial (the blank) in the sample chamber making

9. Press ZERO key.

10. Remove the vial from the sample chamber.

11. Place the vial (the sample) in the sample chamber ma-

12. Press TEST key.

The result is shown in the display in mg/l Chloride.

] key.

sure that the

king sure that the

marks are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. The test sample and the reagents should have room temperature for test performance.

2. The test sample should have a pH of between 3 and 9.

3. Store the reagent bottles in a cool, dry place ideally at between 4°C and 8°C.

4. Interferences: Thiocyanate, Sulﬁde, Thiosulfate, Bromide and Iodide interfere because they react like Chloride.

SP600 Spectrophotometer 01/2008

1.1 Methods

Chlorine with Tablet

0.1 – 6 mg/l Cl

Chlorine with Tablet

0.02 – 0.5 mg/l Cl

Chlorine >> diff free total

>> diff

>> free

>> total

0 0

0 1

1 0

Chlorine with Tablet

0.02 – 3 mg/l Cl

Chlorine with Liquid Reagent

0.02 – 3 mg/l Cl

Chlorine with Powder Pack

0.01 – 2 mg/l Cl

The following selection is shown in the display:

for the differentiated determination of free, combined and total Chlorine

for the determination of free Chlorine

for the determination of total Chlorine

Select the desired determination with the arrow keys

] and [ ]. Conﬁrm with [ ] key.

[

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing

substances, the subsequent determination of Chlorine may show lower results. To avoid any measurement errors, only use glassware free of Chlorine consumption.

Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionized water.

2. For individual testing of free and total Chlorine, the use of different sets of glassware is recommend (EN ISO 7393-2, 5.3)

3. Preparing the sample: When preparing the sample, the escape of Chlorine gases, e.g. by pipetting or

shaking, must be avoided. The analysis must take place immediately after taking the sample.

4. The DPD colour development is carried out at a pH value of 6.3 to 6.5. The reagents therefore contain a buffer for the pH adjustment.

Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the reagent is added (use 0.5 mol/l Sulfuric acid resp. 1 mol/l Sodium hydroxide).

5. Exceeding of the measuring range: Concentrations above 10 mg/l Chlorine using tablets 4 mg/l Chlorine using liquid reagents 2 mg/l using powder packs can produce results within the measuring range up to 0 mg/l. In this event, the

water sample must be diluted with water free of Chlorine. 10 ml of the diluted sample will be mixed with the reagent and the measurement repeated.

6. Turbidity (lead to errors): The use of the DPD No. 1 tablet (method 98, 99, 100) in samples with high Calcium ion

contents* and/or high conductivity* can lead to turbidity of the sample and therefore incorrect measurements. In this event, the reagent tablet DPD No. 1 High Calcium should be used as an alternative. Even if the turbidity does occur after the DPD No. 3 tablet has been added, this can be prevented by using the DPD No. 1 HIGH CALCIUM tablet.

* it is not possible to give exactly values, because the development of

turbidity depends on nature and ingredients of the sample.

7. If ??? is displayed at a differenciated test result see page 325.

Oxidizing agents such as Bromine, Ozone etc. interfere as they react like Chlorine.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

Zero accepted prepare T1 press TEST

T1 accepted prepare T2 press TEST

Countdown 2:00

*,** mg/l free Cl *,** mg/l comb Cl *,** mg/l tot Cl

Chlorine, differentiated determination

with Tablet

0.1 – 6 mg/l Cl

1. Fill a clean 10 mm cell with water sample.

2. Place the cell in the sample chamber making sure that

the positioning is correct.

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the

cell and dry completely.

5. Rinse a beaker with the water sample and empty it,

leaving a few drops remaining in the beaker.

6. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

8. Fill the 10 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that

the positioning is correct.

10. Press TEST key.

11. Remove the cell from the sample chamber and return

the colored test solution completely back into the beaker.

12. Add one DPD No. 3 tablet straight from the foil to the

same water sample and crush the tablet using a clean stirring rod. Dissolve the tablet.

13. Fill the 10 mm cell with the colored test solution.

14. Place the cell in the sample chamber making sure that

the positioning is correct.

15. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display in:

mg/l free Chlorine

mg/l combined Chlorine mg/l total Chlorine

SP600 Spectrophotometer 01/2008

1.1 Methods

Chlorine, free

with Tablet

0.1 – 6 mg/l Cl

1. Fill a clean 10 mm cell with water sample.

2. Place the cell in the sample chamber making sure that the positioning is correct.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the cell and dry completely.

5. Rinse a beaker with the water sample and empty it, leaving a few drops remaining in the beaker.

6. Add one DPD No. 1 tablet straight from the foil and crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

8. Fill the 10 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that the positioning is correct.

10. Press TEST key.

The result is shown in the display in mg/l free Chlorine.

Notes:

see page 51

SP600 Spectrophotometer 01/2008

1.1 Methods

Chlorine, total

with Tablet

0.1 – 6 mg/l Cl

1. Fill a clean 10 mm cell with water sample.

2. Place the cell in the sample chamber making sure that the positioning is correct.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 2:00

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the cell and dry completely.

5. Rinse a beaker with the water sample and empty it, leaving a few drops remaining in the beaker.

6. Add one DPD No. 1 and one DPD No. 3 tablet straight from the foil and crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

8. Fill the 10 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that the positioning is correct.

10. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display in mg/l total Chlorine.

Notes:

see page 51

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

Zero accepted prepare T1 press TEST

T1 accepted prepare T2 press TEST

Countdown 2:00

*,** mg/l free Cl *,** mg/l comb Cl *,** mg/l tot Cl

Chlorine, differentiated determination

with Tablet

0.02 – 0.5 mg/l Cl

1. Fill a clean 50 mm cell with water sample.

2. Place the cell in the sample chamber making sure that the positioning is correct.

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the cell and dry completely.

5. Rinse a beaker with the water sample and empty it, leaving a few drops remaining in the beaker.

6. Add one DPD No. 1 tablet straight from the foil and crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

8. Fill the 50 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that the positioning is correct.

10. Press TEST key.

11. Remove the cell from the sample chamber and return the colored test solution completely back into the beaker.

12. Add one DPD No. 3 tablet straight from the foil to the same water sample and crush the tablet using a clean stirring rod. Dissolve the tablet.

13. Fill the 50 mm cell with the colored test solution.

14. Place the cell in the sample chamber making sure that the positioning is correct.

15. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display in:

mg/l free Chlorine

mg/l combined Chlorine mg/l total Chlorine

SP600 Spectrophotometer 01/2008

1.1 Methods

Chlorine, free

with Tablet

0.02 – 0.5 mg/l Cl

1. Fill a clean 50 mm cell with water sample.

2. Place the cell in the sample chamber making sure that the positioning is correct.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the cell and dry completely.

5. Rinse a beaker with the water sample and empty it, leaving a few drops remaining in the beaker.

6. Add one DPD No. 1 tablet straight from the foil and crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

8. Fill the 50 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that the positioning is correct.

10. Press TEST key.

The result is shown in the display in mg/l free Chlorine.

Notes:

see page 51

SP600 Spectrophotometer 01/2008

1.1 Methods

Chlorine, total

with Tablet

0.02 – 0.5 mg/l Cl

1. Fill a clean 50 mm cell with water sample.

2. Place the cell in the sample chamber making sure that the positioning is correct.

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 2:00

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the cell and dry completely.

5. Rinse a beaker with the water sample and empty it, leaving a few drops remaining in the beaker.

6. Add one DPD No. 1 and one DPD No. 3 tablet straight from the foil and crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

8. Fill the 50 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that the positioning is correct.

10. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display in mg/l total Chlorine

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

0 0

Chlorine, differentiated determination with Tablet

0.02 – 3 mg/l Cl2

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that marks are aligned.

the

3. Press ZERO key.

4. Remove the vial from the sample chamber, empty the

vial leaving a few drops in.

5. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

6. Add water sample to the 10 ml mark.

7. Close the vial tightly with the cap and swirl several

times until the tablet is dissolved.

Zero accepted prepare T1 press TEST

8. Place the vial in the sample chamber making sure that marks are aligned.

the

9. Press TEST key.

10. Remove the vial from the sample chamber.

11. Add one DPD No. 3 tablet straight from the foil to the

same water sample and crush the tablet using a clean stirring rod.

12. Close the vial tightly with the cap and swirl several

times until the tablet is dissolved.

SP600 Spectrophotometer 01/2008

1.1 Methods

13. Place the vial in the sample chamber making sure that the marks are aligned.

T1 accepted prepare T2 press TEST

Countdown 2:00

*,** mg/l free Cl *,** mg/l comb Cl *,** mg/l total Cl

14. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in:

mg/l free Chlorine mg/l combined Chlorine mg/l total Chlorine

Notes:

see page 51

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

0 0

Chlorine, free with Tablet

0.02 – 3 mg/l Cl2

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,

close tightly with the cap.

2. Place the vial in the sample chamber making sure that

marks are aligned.

the

3. Press ZERO key.

4. Remove the vial from the sample chamber, empty the

vial leaving a few drops in.

5. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

6. Add water sample to the 10 ml mark.

7. Close the vial tightly with the cap and swirl several

times until the tablet is dissolved.

Zero accepted prepare Test press TEST

8. Place the vial in the sample chamber making sure that

marks are aligned.

the

9. Press TEST key.

The result is shown in the display in mg/l free Chlorine.

Notes:

see page 51

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

0 0

Chlorine, total with Tablet

0.02 – 3 mg/l Cl

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that the

3. Press ZERO key.

4. Remove the vial from the sample chamber, empty the vial leaving a few drops in.

5. Add one DPD No. 1 tablet and one DPD No. 3 tablet straight from the foil and crush the tablets using a clean stirring rod.

6. Add water sample to the 10 ml mark.

7. Close the vial tightly with the cap and swirl several times until the tablets are dissolved.

marks are aligned.

8. Place the vial in the sample chamber making sure that the

Zero accepted prepare Test press TEST

Countdown 2:00

9. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in mg/l total Chlorine.

Notes:

see page 51

marks are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

0 1

Chlorine, differentiated determination with Liquid Reagent

0.02 – 3 mg/l Cl2

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,

close tightly with the cap.

2. Place the vial in the sample chamber making sure that

the

marks are aligned.

3. Press ZERO key.

4. Remove the vial from the sample chamber and empty

the vial.

5. Fill the vial with the same size drops by holding the

bottle vertically and squeeze slowly:

6 drops of DPD 1 buffer solution

2 drops of DPD 1 reagent solution

6. Add water sample to the 10 ml mark.

Zero accepted prepare T1 press TEST

7. Close the vial tightly with the cap and swirl several

times to mix the contents.

8. Place the vial in the sample chamber making sure that

the

marks are aligned.

9. Press TEST key.

10. Remove the vial from the sample chamber.

11. Add 3 drops of DPD 3 solution to the same water

sample.

12. Close the vial tightly with the cap and swirl several

times to mix the contents.

SP600 Spectrophotometer 01/2008

1.1 Methods

13. Place the vial in the sample chamber making sure that the marks are aligned.

T1 accepted prepare T2 press TEST

Countdown 2:00

*,** mg/l free Cl *,** mg/l comb. Cl *,** mg/l total Cl

Notes:

1. After use replace the bottle caps securely noting the colour coding.

2. Store the reagent bottles in a cool, dry place ideally between 6°C and 10°C.

3. Also see page 51.

14. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in:

mg/l free Chlorine mg/l combined Chlorine mg/l total Chlorine

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

0 1

Chlorine, free with Liquid Reagent

0.02 – 3 mg/l Cl

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,

close tightly with the cap.

2. Place the vial in the sample chamber making sure that

the

3. Press ZERO key.

4. Remove the vial from the sample chamber and empty

the vial.

5. Fill the vial with the same size drops by holding the

bottle vertically and squeeze slowly:

6 drops of DPD 1 buffer solution

2 drops of DPD 1 reagent solution

6. Add water sample to the 10 ml mark.

7. Close the vial tightly with the cap and swirl several

times to mix the contents.

marks are aligned.

8. Place the vial in the sample chamber making sure that

the

marks are aligned.

Zero accepted prepare Test press TEST

Notes (free and total Chlorine):

1. After use replace the bottle caps securely noting the colour coding.

2. Store the reagent bottles in a cool, dry place ideally between 6°C and 10°C.

3. Also see page 51.

9. Press TEST key.

The result is shown in the display in mg/l free Chlorine.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

0 1

Chlorine, total with Liquid Reagent

0.02 – 3 mg/l Cl

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that the

3. Press ZERO key.

4. Remove the vial from the sample chamber and empty the vial.

5. Fill the vial with the same size drops by holding the bottle vertically and squeeze slowly:

6 drops of DPD 1 buffer solution

2 drops of DPD 1 reagent solution

3 drops of DPD 3 solution

6. Add water sample to the 10 ml mark.

marks are aligned.

7. Close the vial tightly with the cap and swirl several times to mix the contents.

8. Place the vial in the sample chamber making sure that the

Zero accepted prepare Test press TEST

Countdown 2:00

9. Press TEST key.

Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

total Chlorine.

marks are aligned.

The result is shown in the display in mg/l

SP600 Spectrophotometer 01/2008

1.1 Methods

1 2

3 4

prepare Zero press ZERO

1 0

Chlorine, differentiated determination with Powder Pack

0.01 – 2 mg/l Cl

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that the

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one VARIO Chlorine FREE-DPD / F10 powder pack straight from the foil to the water sample.

6. Close the vial tightly with the cap and swirl several times to mix the contents (approx. 20 seconds).

7. Place the vial in the sample chamber making sure that the

marks are aligned.

Zero accepted prepare T1 press TEST

8. Press TEST key.

9. Remove the vial from the sample chamber. Empty the vial, rinse vial and cap several times and then ﬁll the vial with 10 ml of water sample.

10. Add one VARIO Chlorine TOTAL-DPD / F10 powder pack straight from the foil to the water sample.

11. Close the vial tightly with the cap and swirl several times to mix the contents (approx. 20 seconds).

SP600 Spectrophotometer 01/2008

1.1 Methods

12. Place the vial in the sample chamber making sure that the marks are aligned.

T1 accepted prepare T2 press TEST

Countdown 3:00

*,** mg/l free Cl *,** mg/l comb. Cl *,** mg/l total Cl

13. Press TEST key.

Wait for a reaction period of 3 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in:

mg/l free Chlorine mg/l combined Chlorine mg/l total Chlorine

Notes:

see page 51

SP600 Spectrophotometer 01/2008

1.1 Methods

1 2

3 4

prepare Zero press ZERO

1 0

Chlorine, free with Powder Pack

0.01 – 2 mg/l Cl

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,

close tightly with the cap.

2. Place the vial in the sample chamber making sure that

the

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one VARIO Chlorine FREE-DPD / F10 powder

pack straight from the foil to the water sample.

6. Close the vial tightly with the cap and swirl several

times to mix the contents (approx. 20 seconds).

7. Place the vial in the sample chamber making sure that

the

marks are aligned.

Zero accepted prepare Test press TEST

8. Press TEST key.

The result is shown in the display in mg/l free Chlorine.

Notes:

see page 51

SP600 Spectrophotometer 01/2008

1.1 Methods

1 2

3 4

prepare Zero press ZERO

1 0

Chlorine, total with Powder Pack

0.01 – 2 mg/l Cl

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that the

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one VARIO Chlorine TOTAL-DPD / F10 powder pack straight from the foil to the water sample.

6. Close the vial tightly with the cap and swirl several time to mix the contents (approx. 20 seconds).

7. Place the vial in the sample chamber making sure that the

marks are aligned.

Zero accepted prepare Test press TEST

Countdown 3:00

8. Press TEST key.

Wait for a reaction period of 3 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in mg/l total Chlorine.

Notes:

see page 51

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

0 5

Ø 16 mm

Chlorine HR (Kl) with Tablet

5 – 200 mg/l Cl

1. Fill a clean vial (16 mm Ø) with 10 ml of the water sample,

close tightly with the cap.

2. Place the vial in the sample chamber making sure that

the marks

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one CHLORINE HR (KI) tablet straight from

the foil to the water sample and crush the tablet using a clean stirring rod.

6. Add one ACIDIFYING GP tablet straight from the

foil to the same water sample and crush the tablet using a clean stirring rod.

are aligned.

Zero accepted prepare Test press TEST

7. Cl o se th e vial t ight ly wi th th e c ap an d swi r l

several times until the tablets are dissolved.

8. Place the vial in the sample chamber making sure

that the marks

9. Press TEST key.

The result is shown in the display in mg/l Chlorine.

are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Oxidizing agents interfere as they react like Chlorine.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

1 9

Chlorine dioxide in absence of Chlorine with Tablet

0.05 – 1 mg/l ClO

1. Fill a clean 50 mm cell with water sample.

2. Place the cell in the sample chamber making sure that

the positioning is correct.

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the

cell and dry completely.

5. Rinse a beaker with the sample and empty it leaving

a few drops in.

6. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

7. Add 10 ml of water sample and dissolve the tablet.

Zero accepted prepare Test press TEST

8. Fill the 50 mm cell with the colored test solution.

9. Place the cell in the sample chamber making sure that

the positioning is correct.

10. Press TEST key.

The result is shown in the display in mg/l

Chlorine dioxide.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing

substances, the subsequent determination of Oxidizing agents (e.g. Chlorine, Bromine) may show lower results. To avoid any measurement errors, only use glassware free of Chlorine consumption.

Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionized water.

2. Preparing the sample: When preparing the sample, the escape of Chlorine dioxide gases, e.g. by

pipetting or shaking, must be avoided. The analysis must take place immediately after taking the sample.

3. The DPD colour development is carried out at a pH value of 6.3 to 6.5. The reagent tablet therefore contains a buffer for the pH adjustment.

Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the tablet is added (use 0.5 mol/l Sulfuric acid resp. 1 mol/l Sodium hydroxide).

4. Turbidity (lead to errors): The use of the DPD No. 1 tablet in samples with high Calcium ion contents* and/or high conductivity* can lead to turbidity of the sample and therefore incorrect measurements. In this event, the reagent tablet DPD No. 1 High Calcium should be used as an alternative. * it is not possible to give exactly values, because the development of

turbidity depends on nature and ingredients of the sample.

5. Exceeding of the measuring range: Concentrations above 19 mg/l Chlorine dioxide can produce results within the

measuring range up to 0 mg/l. In this event, the water sample must be diluted with water free of Chlorine dioxide. 10 ml of the diluted sample will be mixed with the reagent and the measurement repeated.

Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Chlorine

dioxide.

SP600 Spectrophotometer 01/2008

1.1 Methods

Chlorine dioxide >> with Cl without Cl

>> with Cl

>> without Cl

2 0

Chlorine dioxide with Tablet

0.05 – 2.5 mg/l ClO

The following selection is shown in the display:

for the determination of Chlorine dioxide in the presence of Chlorine.

for the determination of Chlorine dioxide in the absence of Chlorine.

Select the desired determination with the arrow keys

] and [ ]. Conﬁrm with [ ] key.

[

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing

substances, the subsequent determination of Chlorine dioxide may show lower results. To avoid any measurement errors, only use glassware free of Chlorine consumption.

Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionized water.

2. Preparing the sample: When preparing the sample, the escape of Chlorine dioxide gases, e.g. by

pipetting or shaking, must be avoided. The analysis must take place immediately after taking the sample.

3. The DPD colour development is carried out at a pH value of 6.3 to 6.5. The reagent tablet therefore contains a buffer for the pH adjustment.

Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the tablet is added (use 0.5 mol/l Sulfuric acid resp. 1 mol/l Sodium hydroxide).

4. Exceeding of the measuring range: Concentrations above 19 mg/l Chlorine dioxide can produce results within the

5. If ??? is displayed at a differentiated test result see page 325.

Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Chlorine dioxide.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

2 0

Chlorine dioxide in the presence of Chlorine with Tablet

0.05 – 2.5 mg/l ClO

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,

close tightly with the cap.

2. Place the vial in the sample chamber making sure that marks are aligned.

the

3. Press ZERO key.

4. Remove the vial from the sample chamber, empty the

vial leaving a few drops in.

5. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

Fill a second clean vial with 10 ml of water sample.

7. Add one GLYCINE tablet straight from the foil and

crush the tablet using a clean stirring rod.

Zero accepted prepare T1 press TEST

8. Cl ose the vi al tig h tly with the ca p a nd sw i rl

several times until the tablet is dissolved.

9. Transfer the contents of the second vial into the

prepared vial.

10. C los e t he vi a l tig h tly with th e cap an d swi r l

several times until the tablet is dissolved.

11. Place the vial in the sample chamber making sure that marks are aligned.

the

12. Press TEST key.

SP600 Spectrophotometer 01/2008

1.1 Methods

13. Remove the vial from the sample chamber. Empty the vial, rinse vial and cap several times and then ﬁll with a 2-3 drops of water sample.

14. Add one DPD No. 1 tablet straight from the foil and crush the tablet using a clean stirring rod.

15. Add water sample to the 10 ml mark.

16. Close the vial tightly with the cap and swirl several times until the tablet is dissolved.

T1 accepted prepare T2 press TEST

T2 accepted prepare T3 press TEST

Countdown 2:00

*,** mg/l ClO2 [Cl]

*,** mg/l ClO2

*,** mg/l free Cl *,** mg/l comb. Cl *,** mg/l total Cl

17. Place the vial in the sample chamber making sure that the

18. Press TEST key.

19. Remove the vial from the sample chamber.

20. Add one DPD No. 3 tablet straight from the foil to the same water sample and crush the tablet using a clean stirring rod.

21. Close the vial tightly with the cap and swirl several times until the tablet is dissolved.

22. Place the vial in the sample chamber making sure that the

23. Press TEST key. Wait for a reaction period of 2 minutes.

After the reaction period is ﬁnished the measurement starts automatically.

The result is shown in the display in:

as Chlorine dioxide in mg/l Chlorine, or as Chlorine dioxide in mg/l ClO2.

mg/l free Chlorine mg/l combined Chlorine mg/l total Chlorine

marks are aligned.

Notes:

See next page.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes: (Chlorine dioxide in the presence of Chlorine)

1. The conversion factor to convert Chlorine dioxide as Chlorine to Chlorine dioxide as ClO2 is approximately 0.4 (more exactly 0.38).

mg/l ClO2 = mg/l ClO2 [Cl] x 0.38 ClO2[Cl] ClO (Chlorine dioxide displayed as Chlorine units ClO2 [Cl] has its origin out of the swimming

poolwater treatment according to DIN 19643.)

2. The total Chlorine result given includes the contribution by the Chlorine dioxide (as Chlorine) reading. For true total Chlorine value subtract the Chlorine dioxide (as Chlorine) reading from the quoted total Chlorine reading.

3. Also see page 75.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

2 0

Chlorine dioxide in absence of Chlorine with Tablet

0.05 – 2.5 mg/l ClO

1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap.

2. Place the vial in the sample chamber making sure that marks are aligned.

the

3. Press ZERO key.

4. Remove the vial from the sample chamber, empty the

vial leaving a few drops in.

5. Add one DPD No. 1 tablet straight from the foil and

crush the tablet using a clean stirring rod.

6. Add water sample to the 10 ml mark.

7. Cl ose the vi al tig h tly with the ca p a nd sw i rl

several times until the tablet is dissolved.

8. Place the vial in the sample chamber making sure that

the

Zero accepted prepare Test press TEST

*,** mg/l ClO2 [Cl]

*,** mg/l ClO2

9. Press TEST key.

The result is shown in the display

as Chlorine dioxide in mg/l Chlorine, or as Chlorine dioxide in mg/l ClO

Notes:

see page 75

marks are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Chrom >> diff Cr (VI) Cr (III + VI)

>> diff

>> Cr (VI)

>> Cr (III + VI)

2 4

2 5

Chromium with Powder Pack

0.005 – 0.5 mg/l Cr / 5 – 500 μg/l Cr

Chromium with Powder Pack

0.02 – 2 mg/l Cr

The following selection is shown in the display:

for the differentiated determination of Chromium (VI), Chromium (III) and total Chromium

for the determination of Chromium (VI)

for the determination of total Chromium (sum Cr (III) + Cr (VI))

Select the desired determination with the arrow keys

] and [ ]. Conﬁrm with the [ ] key.

[

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

2 4

Ø 16 mm

Chromium, differentiated determination with Powder Pack

0.005 – 0.5 mg/l Cr / 5 – 500 μg/l Cr

Digestion:

Fill 10 ml of water sample into a clean vial (16 mm Ø).

2. Add one PERSULF.RGT FOR CR powder pack straight from the foil into the vial.

3. Close the vial tightly with the cap and swirl several times to mix the contents.

4. Heat the vial for 120 minutes in a preheated thermoreactor at a temperature of 100°C.

5. Remove the vial from the thermoreactor. (CAUTION: The vials are hot!). Invert the vial and allow to cool to room temperature.

Performing test procedure:

6. Fill a clean 50 mm cell with deionized water.

7. Place the cell in the sample chamber making sure that the positioning is correct.

8. Press ZERO key.

9. Remove the cell from the sample chamber. Empty the cell and dry completely.

Zero accepted prepare T1 press TEST

Countdown 5:00

10. Add one CHROMIUM HEXAVALENT powder pack straight from the foil into the pre prepared vial (see step 5).

11. Close the vial tightly with the cap and swirl several times to mix the contents.

12. Fill the 50 mm cell with this test solution.

13. Place the cell in the sample chamber making sure that the positioning is correct.

14. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

SP600 Spectrophotometer 01/2008

1.1 Methods

15. Fill a second clean vial (16 mm Ø) with 10 ml of water sample.

16. Add one CHROMIUM HEXAVALENT powder pack straight from the foil to the water sample.

Ø 16 mm

T1 accepted prepare T2 press TEST

Countdown 5:00

*,** mg/l Cr (VI) *,** mg/l Cr (III) *,** mg/l Cr tot.

Notes:

1. Performing steps 1–14 determines concentration of total chromium and steps 15–20 determines concentration of Chromium (VI). The concentration of Chromium (III) results out of the difference.

2. pH value of the water sample should be between 3 and 9.

3. For information about interferences especially in waste water and chemical waste water through metals and reductive or oxidic agents see DIN 38 405 – D 24 and Standard Methods of Water and Wastewater, 20th Edition; 1998.

mg/l

4. μg/l

17. Close the vial tightly with the cap and swirl several times to mix the contents.

18. Fill the 50 mm cell with this test solution.

19. Place the cell in the sample chamber making sure that the positioning is correct.

20. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display in:

mg/l Cr (Vl)

mg/l Cr (lll) mg/l Cr total chromium

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 5:00

2 4

Ø 16 mm

Chromium (Vl) with Powder Pack

0.005 – 0.5 mg/l Cr / 5 – 500 μg/l Cr

1. Fill a clean 50 mm cell with deionized water.

2. Place the cell in the sample chamber making sure that the positioning is correct.

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the cell and dry completely.

Fill 10 ml of water sample into a clean vial (16 mm Ø)

6. Add one CHROMIUM HEXAVALENT powder pack straight from the foil to the water sample.

7. Close the vial tightly with the cap and swirl several times to mix the contents.

8. Fill the 50 mm cell with this test solution.

9. Place the cell in the sample chamber making sure that the positioning is correct.

10. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display as Chromium (Vl).

Notes:

see previous page

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

Zero accepted prepare Test press TEST

Countdown 5:00

2 4

Ø 16 mm

Chromium, total (Cr(lll) + Cr(Vl)) with Powder Pack

0.005 – 0.5 mg/l Cr / 5 – 500 μg/l Cr

Digestion:

Fill a clean vial (16 mm Ø) with 10 ml of the water sam-

1. ple.

2. Add one PERSULF.RGT FOR CR powder pack straight from the foil into the vial.

3. Close the vial tightly with the cap and swirl several times to mix the contents.

4. Heat the vial for 120 minutes in a preheated thermoreactor at a temperature of 100°C.

5. Remove the vial from the thermoreactor. (CAUTION: The vials are hot!). Invert the vial and allow to cool to room temperature.

Performing test procedure:

6. Fill a clean 50 mm cell with deionized water.

7. Place the cell in the sample chamber making sure that the positioning is correct.

8. Press ZERO key.

9. Remove the cell from the sample chamber. Empty the cell and dry completely.

10.

Add one CHROMIUM HEXAVALENT powder pack straight from t he foi l to the pre-prepared water sample.

11. Close the vial tightly with the cap and swirl several times to mix the contents.

12. Fill the 50 mm cell with this test solution.

13. Place the cell in the sample chamber making sure that the positioning is correct.

14. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display as total Chromium.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

2 5

Ø 16 mm

Chromium, differentiated determination with Powder Pack

0.02 – 2 mg/l Cr

Digestion:

Fill a clean vial (16 mm Ø) with 10 ml of the water sam-

1. ple.

2. Add one PERSULF.RGT FOR CR powder pack straight from the foil into the vial.

3. Close the vial tightly with the cap and swirl several times to mix the contents.

4. Heat the vial for 120 minutes in a preheated thermoreactor at a temperature of 100°C.

5. Remove the vial from the thermoreactor. (CAUTION: The vials are hot!). Invert the vial and allow to cool to room temperature.

Performing test procedure:

6. Place the pre prepared vial in the sample chamber making sure that the marks

7. Press ZERO key.

8. Remove the vial from the sample chamber.

are aligned.

Zero accepted prepare T1 press TEST

Countdown 5:00

9. Add one CHROMIUM HEXAVALENT powder pack straight from the foil into the pre prepared vial.

10. Close the vial tightly with the cap and swirl several times to mix the contents.

11. Place the vial in the sample chamber making sure that the marks

12. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Ø 16 mm

T1 accepted prepare T2 press TEST

Countdown 5:00

13. Fill a second clean vial (16 mm Ø) with 10 ml of water sample.

14. Add one CHROMIUM HEXAVALENT powder pack straight from the foil to the water sample.

15. Close the vial tightly with the cap and swirl several times to mix the contents.

16. Place the vial in the sample chamber making sure that the marks

17. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display in:

are aligned.

*,** mg/l Cr (VI) *,** mg/l Cr (III) *,** mg/l Cr tot.

Notes:

1. Performing steps 1–12 determines concentration of total chromium and steps 13–17 determines concentration of Chromium (VI). The concentration of Chromium (III) results out of the difference.

2. pH value of the water sample should be between 3 and 9.

mg/l Cr (Vl)

mg/l Cr (lll) mg/l Cr total Chromium

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

2 5

Ø 16 mm

Chromium (Vl) with Powder Pack

0.02 – 2 mg/l Cr

Fill a clean vial (16 mm Ø) with 10 ml of the water

1. sample.

2. Place the vial in the sample chamber making sure that the marks

3. Press ZERO key.

4. Remove the vial from the sample chamber.

5. Add one CHROMIUM HEXAVALENT powder pack straight from the foil to the water sample.

6. Close the vial tightly with the cap and swirl several times to mix the contents.

7. Place the vial in the sample chamber making sure that the marks

are aligned.

Zero accepted prepare Test press TEST

Countdown 5:00

8. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement

starts automatically.

The result is shown in the display in mg/l Chromium (Vl).

Notes:

see previous page

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

2 5

Ø 16 mm

Chromium, total (Cr(lll) + Cr(Vl)) with Powder Pack

0.2 – 2 mg/l Cr

Digestion:

Fill a clean vial (16 mm Ø) with 10 ml of the water sam-

1. ple.

2. Add one PERSULF.RGT FOR CR powder pack straight from the foil into the vial.

3. Close the vial tightly with the cap and swirl several times to mix the contents.

4. Heat the vial for 120 minutes in a preheated thermoreactor at a temperature of 100°C.

5. Remove the vial from the thermoreactor.

(CAUTION: The vials are hot!).

Invert the vial and allow to cool to room temperature.

Performing test procedure:

6. Place the pre prepared vial in the sample chamber making sure that the marks

7. Press ZERO key.

8. Remove the vial from the sample chamber.

are aligned.

9. Add one CHROMIUM HEXAVALENT powder pack

10. Close the vial tightly with the cap and swirl several

11. Place the vial in the sample chamber making sure that

Zero accepted prepare Test press TEST

Countdown 5:00

12. Press TEST key.

Wait for a reaction period of 5 minutes.

After the reaction period is ﬁnished the measurement

The resul t is shown in the display in mg/l total

straight from the foil to the water sample.

times to mix the contents.

the marks

starts automatically.

Chromium.

are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

3 0

Ø 16 mm

COD LR with Tube Test

0 – 150 mg/l O

1. Open one white capped reaction vial and add 2 ml deionized water (this is the blank (Note 1)).

2. Open another white capped reaction vial and add 2 ml of water sample (this is the sample).

3. Close the vials with the cap tightly. Invert the vial gently several times to mix the contents.

(CAUTION: The vial will become hot during mixing!)

4. Heat the vials for 120 minutes in the preheated reactor at a temperature of 150°C.

5. (CAUTION: The vials are hot!) Remove the tubes from the heating block and allow them to cool to 60°C or less. Mix the contents by carefully inverting each tube several times while still warm. Then allow the tubes to cool to ambient temperature before measuring. (Note 2).

6. Place the vial (the blank (Note 3, 4)) in the sample chamber making sure that the marks

7. Press ZERO key.

are aligned.

Zero accepted prepare Test press TEST

8. Remove the vial from the sample chamber.

9. Place the vial (the sample (Note 3, 4)) in the sample chamber making sure that the marks

10. Press TEST key.

The result is shown in the display in mg/l COD.

are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Run samples and blanks with the same batch of vials. The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch.

2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for ﬁnal measurements.

3. Suspended solids in the vial lead to incorrect measurements. For this reason it is important to place the vials carefully in the sample chamber. The precipitate at the bottom of the sample should be not suspended.

4. Clean the outside of the vials with a towel. Finger prints or other marks will be removed.

5. Samples can be measured when the Chloride contents does not exceed 1000 mg/l.

6. In exceptional cases, compounds contained in the water cannot be oxidized adequate, what results in minimum ﬁndings, compared with the reference method.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

3 1

Ø 16 mm

COD MR with Tube Test

0 – 1 500 mg/l O

1. Open one white capped reaction vial and add 2 ml deionized water (this is the blank (Note 1)).

2. Open another white capped reaction vial and add 2 ml of water sample (this is the sample).

3. Close the vials with the cap tightly. Invert the vial gently several times to mix the contents.

(CAUTION: The vial will become hot during mixing!)

4. Heat the vials for 120 minutes in the preheated reactor at a temperature of 150°C.

6. Place the vial (the blank (Note 3, 4)) in the sample chamber making sure that the marks

7. Press ZERO key.

are aligned.

Zero accepted prepare Test press TEST

8. Remove the vial from the sample chamber.

9. Place the vial (the sample (Note 3, 4)) in the sample chamber making sure that the marks

10. Press TEST key.

The result is shown in the display in mg/l COD.

are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Run samples and blanks with the same batch of vials. The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch.

2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for ﬁnal measurements.

4. Clean the outside of the vials with a towel. Finger prints or other marks will be removed.

5. Samples can be measured when the Chloride contents does not exceed 1000 mg/l.

6. In exceptional cases, compounds contained in the water cannot be oxidized adequate, what results in minimum ﬁndings, compared with the reference method.

7. For samples under 100 mg/l COD it is recommendable to repeat the test with the tube test for COD LR.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

3 2

Ø 16 mm

COD HR with Tube Test

0 – 15 g/l O2 (=^ 0 – 15 000 mg/l O2)

1. Open one white capped reaction vial and add 0.2 ml deionized water (this is the blank (Note 1)).

2. Open another white capped reaction vial and add

0.2 ml of water sample (this is the sample).

3. Close the vials with the cap tightly. Invert the vial gently several times to mix the contents.

(CAUTION: The vial will become hot during mixing!)

4. Heat the vials for 120 minutes in the preheated reactor at a temperature of 150°C.

6. Place the vial (the blank (Note 3, 4)) in the sample chamber making sure that the marks

7. Press ZERO key.

are aligned.

Zero accepted prepare Test press TEST

8. Remove the vial from the sample chamber.

9. Place the vial (the sample (Note 3, 4)) in the sample chamber making sure that the marks

10. Press TEST key.

The result is shown in the display in g/l COD.

are aligned.

SP600 Spectrophotometer 01/2008

1.1 Methods

Notes:

1. Run samples and blanks with the same batch of vials. The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch.

2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for ﬁnal measurements.

4. Clean the outside of the vials with a towel. Finger prints or other marks will be removed.

5. Samples can be measured when the Chloride contents does not exceed 1000 mg/l.

6. In exceptional cases, compounds contained in the water cannot be oxidized adequate, what results in minimum ﬁndings, compared with the reference method.

7. For samples under 1 g/l COD it is recommendable to repeat the test with the test kit for COD MR or for samples under 0.1 g/l COD with the tube test COD LR.

SP600 Spectrophotometer 01/2008

1.1 Methods

Copper >> diff free total

>> diff

>> free

>> total

4 9

5 0

Copper with Tablet

0.05 – 1 mg/l Cu

Copper with Tablet

0.5 – 5 mg/l Cu

The following selection is shown in the display:

for the differentiated determination of free, combined and total Copper.

for the determination of free Copper.

for the determination of total Copper.

Select the desired determination with the arrow keys

] and [ ]. Conﬁrm with [ ] key.

[

SP600 Spectrophotometer 01/2008

1.1 Methods

Note:

1. If ??? is displayed at the diffentiated test result see page 325.

SP600 Spectrophotometer 01/2008

1.1 Methods

prepare Zero press ZERO

4 9

Copper, differentiated determination with Tablet

0.05 – 1 mg/l Cu

1. Fill a clean 50 mm cell with water sample.

2. Place the cell in the sample chamber making sure that the positioning is correct.

3. Press ZERO key.

4. Remove the cell from the sample chamber. Empty the cell and dry completely.

5. Fill a beaker with 10 ml of water sample.

6. Add one COPPER No. 1 tablet straight from the foil and crush the tablet using a clean stirring rod. Dissolve the tablet.

7. Fill the 50 mm cell with the colored test solution.

Zero accepted prepare T1 press TEST

8. Place the cell in the sample chamber making sure that the positioning is correct.

9. Press TEST key.

10. Remove the cell from the sample chamber and return the colored test solution completely back into the beaker.

12. Add one COPPER No. 2 tablet straight from the foil to the same water sample and crush the tablet using a clean stirring rod. Dissolve the tablet.

ORBECO SP600 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual