MC500
tm
Multi-Parameter
Colorimeter
Instruction Manual
1
MC500 Colorimeter 06-2010
Safety precautions
CAUTION
Reagents are formulated exclusively for chemical analysis and must not be used for
any other purpose. Keep reagents out of reach of children. Some reagents contain
compounds which are not entirely harmless environmentally. Be aware of the
ingredients and take proper care when disposing of the test solution.
CAUTION
Please read this instruction manual before unpacking, setting up or using the
colorimeter. Please read the method description completely before performing the test.
Be aware of the risks of using the required reagents by reading the MSDS (Material Safety
Data Sheets). Failure could result in serious injury to the operator or damage to the
instrument.
MSDS: www.orbeco.com
CAUTION
The accuracy of the instrument is only valid if the instrument is used in an
environment with controlled electromagnetic disturbances according to DIN 61326.
Wireless devices, e.g. wireless phones, should not be used near the instrument.
Revision_2d 06/2010
2
MC500 Colorimeter 06-2010
Table of contents
Part 1 METHODS
...................................................................................................... 7
1.1 Table of Methods ............................................................................................................................................... 8
Acid demand to pH 4.3.........................................................................................................................................12
Alkalinity-total (Alkalinity-m, m-Value) ..........................................................................................................14
Alkalinity-p (p-value) .............................................................................................................................................16
Aluminum with tablets.........................................................................................................................................18
Aluminum (powder pack) ...................................................................................................................................20
Ammonia with tablets ..........................................................................................................................................22
Ammonia (powder pack) .....................................................................................................................................24
Ammonia, low range (LR) ....................................................................................................................................26
Ammonia, high range (HR) .................................................................................................................................28
Boron ..........................................................................................................................................................................30
Bromine .....................................................................................................................................................................32
Chloramines, (Mono) and Free Ammonia ..................................................................................................... 34
Chloride .....................................................................................................................................................................38
Chlorine .....................................................................................................................................................................40
Chlorine with tablet
dierentiated determination (free, combined, total) ...........................................................................42
free Chlorine........................................................................................................................................................44
total Chlorine ......................................................................................................................................................45
Chlorine with liquid reagent
dierentiated determination (free, combined, total) ...........................................................................46
free Chlorine........................................................................................................................................................48
total Chlorine ......................................................................................................................................................49
Chlorine (powder pack)
dierentiated determination (free, combined, total) ...........................................................................50
free Chlorine........................................................................................................................................................52
total Chlorine ......................................................................................................................................................53
Chlorine dioxide .....................................................................................................................................................54
in presence of Chlorine ...................................................................................................................................56
in absence of Chlorine .....................................................................................................................................59
Chlorine HR (Kl) ....................................................................................................................................................... 60
Chlorine UHR..............................................................................................................See Sodium Hypochlorite
COD, low range (LR) ...............................................................................................................................................62
COD, middle range (MR) ......................................................................................................................................64
COD, high range (HR) ............................................................................................................................................66
Color ............................................................................................................................................................................68
Copper with tablet .................................................................................................................................................70
dierentiated determination (free, combined, total) ...........................................................................71
free Copper ..........................................................................................................................................................72
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MC500 Colorimeter 06-2010
total Copper ........................................................................................................................................................ 73
Copper PP .................................................................................................................................................................74
Cyanide ......................................................................................................................................................................76
Cyanuric acid ............................................................................................................................................................78
DEHA T ........................................................................................................................................................................80
DEHA PP .....................................................................................................................................................................82
Fluoride ......................................................................................................................................................................84
Hardness, Calcium (using Calcheck Tablets) ................................................................................................. 86
Hardness, Calcium (using Calico Tablets) .......................................................................................................88
Hardness, total .........................................................................................................................................................90
Hardness, total HR ..................................................................................................................................................92
Hydrazine ..................................................................................................................................................................94
Hydrazine with liquid reagent ...........................................................................................................................96
Hydrazine with Vacu-vials ...................................................................................................................................98
Hydrogen peroxide ............................................................................................................................................. 100
Iodine ....................................................................................................................................................................... 102
Iron ........................................................................................................................................................................... 104
Iron with tablet................................................................................................................................................ 106
Iron (powder packs)....................................................................................................................................... 108
Iron (TPTZ) (powder packs)......................................................................................................................... 110
Mangenese with tablet ..................................................................................................................................... 112
Manganese LR (powder packs) ...................................................................................................................... 114
Manganese HR (powder packs) ...................................................................................................................... 116
Molybdate with tablet ....................................................................................................................................... 118
Molybdate HR (powder pack) ......................................................................................................................... 120
Nitrate ...................................................................................................................................................................... 122
Nitrite with tablet ................................................................................................................................................ 124
Nitrite LR (powder pack) ................................................................................................................................... 126
Nitrogen, total LR ................................................................................................................................................ 128
Nitrogen, total HR................................................................................................................................................ 130
Oxygen, active ...................................................................................................................................................... 132
Oxygen, dissolved ............................................................................................................................................... 134
Ozone ...................................................................................................................................................................... 136
in presence of Chlorine ................................................................................................................................ 138
in absence of Chlorine .................................................................................................................................. 140
PHMB (Biguanide) ............................................................................................................................................... 142
Phosphate .............................................................................................................................................................. 144
Phosphate, ortho LR with tablet ............................................................................................................... 146
Phosphate, ortho HR with tablet .............................................................................................................. 148
Phosphate, ortho (powder packs) ............................................................................................................ 150
Phosphate, ortho (tube test) ...................................................................................................................... 152
Phosphate 1, ortho C .................................................................................................................................... 154
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MC500 Colorimeter 06-2010
Phosphate 2, ortho C .................................................................................................................................... 156
Phosphate, hydrolysable (tube test)........................................................................................................ 158
Phosphate, total (tube test) ........................................................................................................................ 160
Phosphonates (Persulfate UV Oxidation Method) .................................................................................. 162
pH-Value LR with tablet .................................................................................................................................... 166
pH-Value with tablet .......................................................................................................................................... 168
pH-Value with liquid reagent .......................................................................................................................... 170
pH-Value HR with tablet.................................................................................................................................... 172
Potassium ............................................................................................................................................................... 174
Silica .........................................................................................................................................................................176
Silica LR PP ............................................................................................................................................................. 178
Silica HR PP ............................................................................................................................................................ 180
Sodium hypochlorite ......................................................................................................................................... 182
Sulfate T .................................................................................................................................................................. 184
Sulfate (powder pack) ........................................................................................................................................ 186
Sulde ...................................................................................................................................................................... 188
Sulte ....................................................................................................................................................................... 190
Suspended Solids ................................................................................................................................................ 192
Turbidity ................................................................................................................................................................. 194
Urea .......................................................................................................................................................................... 196
Zinc ........................................................................................................................................................................... 198
1.2 Important notes............................................................................................................................. 200
1.2.1 Correct use of reagents ............................................................................................................... 200
1.2.2 Cleaning vials and accessories for analysis .......................................................................... 201
1.2.3 Guidelines for photometric measurements ........................................................................ 201
1.2.4 Sample dilution teqniques ........................................................................................................ 203
1.2.5 Correcting for volume additions ............................................................................................. 203
Part 2 INSTRUMENT MANUAL ....................................................................... 205
2.1 Operation ......................................................................................................................................... 206
2.1.1 Commissioning .............................................................................................................................. 206
2.1.2 Saving data – Important Notes ................................................................................................ 206
2.1.3 Replacement of batteries ........................................................................................................... 206
2.1.4 Instrument (explosion drawing) .............................................................................................. 207
2.2 Overview of function keys ......................................................................................................... 208
2.2.1 Overview .......................................................................................................................................... 208
2.2.2 Displaying time and date ........................................................................................................... 209
2.2.3 User-countdown ........................................................................................................................... 209
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MC500 Colorimeter 06-2010
2.3 Operation mode ............................................................................................................................ 210
2.3.1 Automatic switch o .................................................................................................................... 210
2.3.2 Selecting a method ...................................................................................................................... 210
2.3.2.1 Method-information .................................................................................................................... 210
2.3.2.2 Chemical Species Information ................................................................................................. 211
2.3.3 Dierentiation ................................................................................................................................ 211
2.3.4 Performing Zero ............................................................................................................................ 211
2.3.5 Performing Test .............................................................................................................................. 212
2.3.6 Ensuring reaction periods (countdown) ............................................................................... 212
2.3.7 Changing chemical species ....................................................................................................... 213
2.3.8 Storing results ................................................................................................................................ 213
2.3.9 Printing results ............................................................................................................................... 214
2.3.10 Perform additional measurements ......................................................................................... 214
2.3.11 Selecting a new method ............................................................................................................ 215
2.3.12 Measure absorbance.................................................................................................................... 215
2.4 Photometer settings <Mode-Menu>. .................................................................................. 216
2.4.1 blank because of technical requirements
2.4.2 Instrument basic settings 1 ....................................................................................................... 217
2.4.3 Printing of stored results ............................................................................................................ 221
2.4.4 Recall / delete stored results ..................................................................................................... 226
2.4.5 Calibration ....................................................................................................................................... 231
2.4.6 Lab function .................................................................................................................................... 239
2.4.7 User operations .............................................................................................................................. 240
2.4.8 Special functions ........................................................................................................................... 250
2.4.9 Instrument basic settings 2 ....................................................................................................... 252
2.4.10 Instrument special functions / Service .................................................................................. 252
2.5 Data transfer ...................................................................................................................................253
2.5.1 Print of data ..................................................................................................................................... 253
2.5.2 Data transfer to a personal computer (PC) .......................................................................... 253
2.5.3 Internet-Updates ........................................................................................................................... 253
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MC500 Colorimeter 06-2010
Part 3 SHIPPING CONTENTS ...........................................................................255
3.1 Unpacking ....................................................................................................................................... 256
3.2 Delivery content ............................................................................................................................ 256
3.3 blank because of technical requirements
3.4 Technical data................................................................................................................................. 257
3.5 Abbreviations ................................................................................................................................. 258
3.6 Troubleshooting ............................................................................................................................ 259
3.6.1 Operating messages in the display / error display ............................................................ 259
3.6.2 General problems ......................................................................................................................... 262
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MC500 Colorimeter 06-2010
Part 1
Methods
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MC500 Colorimeter 06-2010
1.1 Methods
1.1 Table of Methods
No. Analysis Reagent Range Displayed asMethod
[nm]
Page
20 Acid demand to
pH 4.3 T
tablet 0.1-4 mmol/l Acid/Indicator
1,2,5
610 12
30
Alkalinity, total T
tablet 5-200 mg/l CaCO3Acid/Indicator
1,2,5
610 14
35 Alkalinity-p T tablet 5-300 mg/l CaCO
3
Acid/Indicator
1,2,5
560 16
40 Aluminum T tablet 0.01-0.3 mg/l Al Eriochrome
Cyanine R
2
530 18
50 Aluminum PP PP + liquid 0.01-0.25 mg/l Al Eriochrome
Cyanine R
2
530 20
60 Ammonium T tablet 0.02-1 mg/l N
Indophenol blue
2,3
610 22
62 Ammonium PP PP 0.01-0.8 mg/l N Salicylate
2
660 24
65
Ammonium LR TT
tube test 0.02-2.5 mg/l N Salicylate
2
660 26
66
Ammonium HR TT
tube test 1-50 mg/l N Salicylate
2
660 28
85 Boron T tablet 0.1-2 mg/l B Azomethine
3
430 30
80 Bromine T tablet 0.05-13 mg/l Br
2
DPD
5
530 32
63 Chloramine,
Mono, PP
PP + liquid 0.04-4.5 mg/l Cl
2
Indophenol 660 34
90 Chloride T tablet 0.5 -25 mg/l Cl Silver nitrate/
turbidity
530 38
100 Chlorine T * tablet 0.01-6 mg/l Cl
2
DPD
1,2,3
530 40, 42
101 Chlorine L * liquid 0.02-4 mg/l Cl
2
DPD
1,2,3
530 40, 46
110 Chlorine PP * PP 0.02-2 mg/l Cl
2
DPD
1,2
530 40, 50
120 Chlorine dioxide T tablet 0.05-11 mg/l ClO
2
DPD, Glycine
1,2
530 54
105 Chlorine HR (Kl) T tablet 5-200 mg/l Cl
2
KI/Acid
5
530 60
130 COD LR TT tube test 0 -150 mg/l O
2
Dichromate/H2SO
4
1,2
430 62
131 COD MR TT tube test 0 -1500 mg/l O
2
Dichromate/H2SO
4
1,2
610 64
132 COD HR TT tube test 0 -15 g/l O
2
Dichromate/H2SO
4
1,2
610 66
204 Color direct
reading
0-500 mg/l Pt. Co. Pt-Co-Scale
1,2
(APHA)
430 68
150 Copper T * tablet 0.05-5 mg/l Cu Biquinoline
4
560 70
153 Copper PP PP 0.05-5 mg/l Cu Bicinchoninate 560 74
157 Cyanide Powder +
liquid
0.01-0.5 mg/l CN Pyridine-barbituric
acid
1
580 76
160
Cyanuric acid T
tablet 2-160 mg/l Cys Melamine 530 78
165 DEHA T tablet +
liquid
20-500 μg/l DEHA PPST
3
560 80
167 DEHA PP PP + liquid 20-500 μg/l DEHA PPST
3
560 82
170 Fluoride L liquid 0.05-2 mg/l F
SPADNS
2
580 84
190 Hardness, Calcium Ttablet 50-900 mg/l CaCO
3
Murexide
4
560 86
* = free, combined, total; PP = powder pack; T = tablet;
L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range;
Vacu-vial® is a registered trade mark of CHEMetrics Inc.
9
MC500 Colorimeter 06-2010
1.1 Methods
1.1 Table of Methods
No. Analysis Reagent Range Displayed asMethod
[nm]
Page
191 Hardness, Calcium 2Ttablet 0-500 mg/l CaCO3Murexide
4
560 88
200 Hardness, total T tablet 2-50 mg/l CaCO
3
Metallphthalein
3
560 90
201 Hardness, total HR T tablet 20-500 mg/l CaCO
3
Metallphthalein
3
560 92
205 Hydrazine P powder 0.05-0.5 mg/l N
2H4
4-(Dimethylamino)benzaldehyde
3
430 94
206 Hydrazine L liquid 0.005-0.6 mg/l N
2H4
4-(Dimethylamino)benzaldehyde
3
430 96
207 Hydrazine C Vacu-vial 0.01-0.7 mg/l N
2H4
PDMAB 430 98
210 Hydrogen
peroxide
tablet 0.03-3 mg/l H
2O2
DPD/catalyst
5
530 100
215 Iodine T tablet 0.05-3.6 mg/l I DPD
5
530 102
220 Iron T tablet 0.02-1 mg/l Fe PPST
3
560 104, 106
222 Iron PP PP 0.02-3 mg/l Fe 1,10-Phenan-
troline
3
530 104, 108
223 Iron (TPTZ) PP PP 0.02-1.8 mg/l Fe TPTZ 580 104, 110
240 Manganese T tablet 0.2-4 mg/l Mn Formaldoxime 530 112
242 Manganese LR PP PP + liquid 0.01-0.7 mg/l Mn PAN 560 114
243 Manganese HR PP PP + liquid 0,1-18 mg/l Mn Periodate
oxidation
2
530 116
250 Molybdate T tablet 1-50 mg/l MoO
4
Thioglycolate
4
430 118
252 Molybdate HR PP PP 0.5-66 mg/l MoO
4
Mercaptoacetic
acid
430 120
265 Nitrate TT tube test 1-30 mg/l N
Chromotropic acid
430 122
270 Nitrite T tablet 0.01-0.5 mg/l N N-(1-Naphthyl)-
ethylendiamine
2,3
560 124
272 Nitrite LR PP PP 0.01-0.3 mg/l N Diazotization 530 126
280 Nitrogen,
total
LR TT
tube test 0.5-25 mg/l N Persulfate
digestion method
430 128
281 Nitrogen,
total
HR TT
tube test 5-150 mg/l N Persulfate
digestion method
430 130
290
Oxygen, active T
tablet 0.1-10 mg/l O
2
DPD 530 132
292 Oxygen, dissolved Vacu-vial 10-800 μg/l O
2
Rhodazine D
TM
530 134
300
Ozone (DPD) T
tablet 0.02-2 mg/l O
3
DPD/Glycine
5
530 136
70 PHMB T tablet 2-60 mg/l PHMB Buer/Indicator 560 142
320
Phosphate, T ortho LRtablet 0.05-4 mg/l PO
4
Ammoniummolybdate
2,3
660 144, 146
* = free, combined, total; PP = powder pack; T = tablet;
L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range;
Vacu-vial® is a registered trade mark of CHEMetrics Inc.
10
MC500 Colorimeter 06-2010
No. Analysis Reagent Range Displayed asMethod
[nm]
Page
321 Phosphate, ortho
HR T
tablet 1-80 mg/l PO
4
Vanando
-
molybdate
2
430 144, 148
323 Phosphate, PP
ortho
PP 0.06-2.5 mg/l PO
4
Ascorbic acid
2
660 144, 150
324 Phosphate,
ortho TTtube test 0.06-5 mg/l PO
4
Ascorbic acid
2
660 144, 152
327 Phosphate 1 C,
ortho
Vacu-vial 5-40 mg/l PO
4
Vanadomolybdate
2
430 144, 154
328 Phosphate 2 C,
ortho
Vacu-vial 0.05-5 mg/l PO
4
Stannous
chloride
2
660 144, 156
325 Phosphate, hydr. TTtube test 0.02-1.6 mg/l P
Acid digestion,
Ascorbic acid
2
660 144, 158
326 Phosphate, total TTtube test 0.02-1.1 mg/l P
Acid persulf digestion,
Ascorbic acid
2
660 144, 160
316 Phosphonate PP PP 0-125 mg/l
Persulfate UVOxidation
660 162
329
pH-Value LR T
tablet 5.2-6.8 —
Bromocresolpurple5560 166
330 pH-Value T tablet 6.5-8.4 — Phenolred
5
560 168
331 pH-Value L liquid 6.5-8.4 — Phenolred
5
560 170
332
pH-Value HR T
tablet 8.0-9.6 — Thymolblue
5
560 172
340 Potassium T tablet 0.7-12 mg/l K
TetraphenylborateTurbidity
4
430 174
350 Silica T tablet 0.05-4 mg/l SiO
2
Silicomolybdate
2,3
660 176
351 Silica LR PP PP 0.1-1.6 mg/l SiO
2
Heteropolyblue
2
660 178
352 Silica HR PP PP 1-90 mg/l SiO
2
Silicomolybdate
2
430 180
212 Sodium
hypochlorite T
tablet 0.2-16 % NaOCl Potassium iodide
5
530 182
355 Sulfate T tablet 5-100 mg/l SO
4
BariumsulfateTurbidity
610 184
360 Sulfate PP PP 5-100 mg/l SO
4
BariumsulfateTurbidity
2
530 186
365 Sulde tablet 0.04-0.5 mg/l S DPD/Catalyst
3,4
660 188
370 Sulte T tablet 0.1-5 mg/l SO
3
DTNB 430 190
384 Suspended Solids Direct
Reading
0-750 mg/l TSS photometric 660 192
386 Turbidity Direct
Reading
0-1000 FAU Attenuated
Radiation Method
530 194
390 Urea T tablet +
liquid
0.1-2.5 mg/l Urea Indophenol/
Urease
610 196
400 Zinc T tablet 0.02 -1 mg/l Zn Zincon
3
610 198
1.1 Methods
1.1 Table of Methods
* = free, combined, total; PP = powder pack; T = tablet;
L = liquid; TT = tube test; LR = low range; MR = middle range; HR = high range;
Vacu-vial® is a registered trade mark of CHEMetrics Inc.
11
MC500 Colorimeter 06-2010
1.1 Methods
Literature
The reagent formulations are based on internationally recognized test methods. Some are
described in national and/or international guidelines.
1) Unied German Procedures for the Analysis of Water and Wastewater
2) Standard Methods for the Examination of Water and Wastewater; 18th Edition, 1992
3) Photometric Analysis Procedures, Schwedt, Scientic Publishing Company Society,
Stuttgart, 1989
4) Photometric Analysis, Lange / Vejdelek, 1980
5) Colorimetric Chemical Analytical Methods, 9th Edition, London
Notes for searching:
Active Oxygen -> Oxygen, active
Alkalinity-m -> Alkalinity, total
Alkalinity, total -> Alkalinity, total
Biguanide -> PHMB
Calcium Hardness -> Hardness, Calcium
Monochloramine -> Chloramine, Mono
Total Hardness -> Hardness, total
m-Value -> Alkalinity, total
p-Value -> Alkalinity-p
Silicon dioxide -> Silica
total Alkalinity -> Alkalinity, total
total Hardness -> Hardness, total
Langelier Saturation -> Mode function 70
Index (Water Balance)
Important Notes for use of this manual - PLEASE READ:
1) The Method number for each test is indicated in the large circles at the beginning of each
procedure. Here is an example:
2
0
2) The Symbol "Ø" signies the OUTSIDE Diameter of the Sample Vial
3) Please note the appropriate reagent platform (tablet, powder, vial, etc.) for each test.
4) Be sure to observe the proper reagent reaction time indicated for each procedure for accurate
results.
12
MC500 Colorimeter 06-2010
1.1 Methods
Acid demand to pH 4.3
with Tablet
0.1 – 4 mmol/l
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one ALKA-M-PHOTOMETER tablet straight
from the foil to the water sample and crush the tablet
using a clean stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
The result is shown in the display as Acid demand to
pH 4.3 in mmol/l.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
2
0
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MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. The terms total Alkalinity, Alkalinity-m, m-Value and Acid demand to pH 4.3 are
identical.
2. For accurate results, be sure to test on exactly 10 ml of water sample .
14
MC500 Colorimeter 06-2010
1.1 Methods
Alkalinity, total = Alkalinity-m =
m-Value
with Tablet
5 – 200 mg/l CaCO
3
1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,
close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one ALKA-M-PHOTOMETER tablet straight
from the foil to the water sample and crush the tablet
using a clean stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
The result is shown in the display as total Alkalinity.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
3
0
15
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. The terms total Alkalinity, Alkalinity-m, m-Value and Alkalinity to pH 4.3 are identical.
2. For accurate results exactly 10 ml of water sample must be taken for the test.
3. Conversion table:
Acid demand to pH 4.3
DIN 38 409 (KS4.3)
German
°dH*
English
°eH*
French
°fH*
1 mg/l CaCO
3
0.02 0.056 0.07 0.1
*Carbonate hardness (reference = Hydrogen carbonate-anions)
Example:
10 mg/l CaCO
3
= 10 mg/l x 0.056 = 0.56 °dH
10 mg/l CaCO3 = 10 mg/l x 0.02 = 0.2 mmol/l
4.
CaCO
3
°dH
°eH
°fH
°aH
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MC500 Colorimeter 06-2010
1.1 Methods
Alkalinity-p = p-value
with Tablet
5 – 300 mg/l CaCO
3
1.
Fill a clean vial (24 mm Ø) with 10 ml of the water
sample,
close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one ALKA-P-PHOTOMETER tablet stra ight
from the foil to the water sample and crush the tablet
using a clean stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
7. Place the vial in the sample chamber making sure
that the
marks are aligned.
8. Press TEST key.
The result is shown in the display as Alkalinity-p.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
3
5
17
MC500 Colorimeter 06-2010
CaCO
3
°dH
°eH
°fH
°aH
5. By determining Alkalinity-p and Alkalinity-m it is possible to classify the alkalinity as
Hydroxide, Carbonate and Hydrogencarbonate.
The following dierentiation is only valid if:
a) no other alkalis are present and
b) Hydroxide and Hydrogen are not present in the same water sample.
Case 1: Alkalinity-p = 0
Hydrogen carbonate = m
Carbonate = 0
Hydroxide = 0
Case 2: Alkalinity-p > 0 and Alkalinity-m > 2p
Hydrogen carbonate = m – 2p
Carbonate = 2p
Hydroxide = 0
Case 3: Alkalinity-p > 0 and Alkalinity-m < 2p
Hydrogen carbonate = 0
Carbonate = 2m – 2p
Hydroxide = 2p – m
1.1 Methods
Notes
1. The terms Alkalinity-p, p-Value and Alkalinity to pH 8.2 are identical.
2. For accurate test results exactly 10 ml of water sample must be taken for the test.
3. This method was developed from a volumetric procedure for the determination of
Alkalinity-p. Due to undened conditions, the deviations from the standardized method
may be greater.
4. Conversion table:
mg/l CaCO
3
°dH °fH °eH
1 mg/l CaCO
3
---- 0.056 0.10 0.07
1 °dH 17.8 ---- 1.78 1.25
1 °fH 10.0 0.56 ---- 0.70
1 °eH 14.3 0.80 1.43 ----
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MC500 Colorimeter 06-2010
1.1 Methods
Aluminum
with Tablet
0.01 – 0.3 mg/l Al
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one ALUMINUM No. 1 tablet straight from the
foil to the water sample and crush the tablet using a
clean stirring rod (dissolve the tablet).
6. Add one ALUMINUM No. 2 tablet straight from the
foil to the same water sample and crush the tablet using
a clean stirring rod.
7. Close the vial tightly with the cap and swirl gently several
times until the tablets are dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
Wait for a reaction period of 5 minutes.
After the reaction period is nished the measurement starts
automatically.
The result is shown in the display in mg/l Aluminum.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
5:00
4
0
19
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Before using clean the vials and the measuring beaker with Hydrochloric acid
(approx. 20%). Rinse then thoroughly with deionized water.
2. To get accurate results the sample temperature must be between 20°C and 25°C.
3. A low test result may be given in the presence of Fluorides and Polyphosphates.
The eect of this is generally insignicant unless the water has uoride added articially.
In this case. the following table should be used:
Fluoride
[mg/l F]
Displayed value: Aluminum [mg/l Al]
0.05 0.10 0.15 0.20 0.25 0.30
0.2 0.05 0.11 0.16 0.21 0.27 0.32
0.4 0.06 0.11 0.17 0.23 0.28 0.34
0.6 0.06 0.12 0.18 0.24 0.30 0.37
0.8 0.06 0.13 0.20 0.26 0.32 0.40
1.0 0.07 0.13 0.21 0.28 0.36 0.45
1.5 0.09 0.20 0.29 0.37 0.48 ---
Example: If the result of Aluminum determination is 0.15 mg/l Al and the Fluoride
concentration is known to be 0.4 mg/l F. the true concentration of Aluminum is
0.17 mg/l Al.
4.
Al
Al2O
3
20
MC500 Colorimeter 06-2010
1.1 Methods
Aluminum
with Powder Pack
0.01 – 0.25 mg/l Al
Use two clean vials (24 mm Ø) and mark one as blank
for zeroing.
1. Fill 20 ml of the water sample in a 100 ml beaker.
2.
Add the contents of one Aluminum ECR F20
Powder Pack straight from the foil to the water sample.
3. Dissolve the powder using a clean stirring rod.
4. Press [
] key.
Wait for a reaction period of 30 seconds.
After the reaction period is nished proceed as follows:
5.
Add
the contents of
one Hexamine F20 Powder
Pack straight
from the foil to the same water sample.
6. Dissolve the powder using a clean stirring rod.
7. A d d 1 drop of Aluminum ECR Masking
Reagent in the vial marked as blank.
8. Add 10 ml of the prepared water sample to the vial (this
is the blank).
9. Add the remaining 10 ml of the prepared water sample
in the second clean vial (this is the sample).
10. Close the vials tightly with the caps and swirl several
times to mix the contents.
11. Press [
] key.
Wait for a reaction period of 5 minutes.
Countdown 1
0:30
start:
Countdown 2
5:00
start:
1 2
3 4
5
0
21
MC500 Colorimeter 06-2010
1.1 Methods
After the reaction period is nished proceed as follows:
12. Place the vial (the blank) in the sample chamber
making sure that the marks are aligned.
13. Press ZERO key.
14. Remove the vial from the sample chamber.
15. Place the vial (the sample) in the sample chamber
making sure that the
marks are aligned.
16. Press TEST key.
The result is shown in the display in mg/l Aluminum.
Notes:
1. Before using clean the vials and the measuring beaker with Hydrochloric acid
(approx. 20%). Rinse then thoroughly with deionized water.
2. To get accurate results the sample temperature must be between 20°C and 25°C.
3. A low test result may be given in the presence of Fluorides and Polyphosphates.
The eect of this is generally insignicant unless the water has uoride added articially.
In this case, the following table should be used:
Fluoride
[mg/l F]
Displayed value: Aluminum [mg/l Al]
0.05 0.10 0.15 0.20 0.25 0.30
0.2 0.05 0.11 0.16 0.21 0.27 0.32
0.4 0.06 0.11 0.17 0.23 0.28 0.34
0.6 0.06 0.12 0.18 0.24 0.30 0.37
0.8 0.06 0.13 0.20 0.26 0.32 0.40
1.0 0.07 0.13 0.21 0.28 0.36 0.45
1.5 0.09 0.20 0.29 0.37 0.48 ---
Example: If the result of Aluminum determination is 0.15 mg/l Al and the Fluoride
concentration is known to be 0.4 mg/l F, the true concentration of Aluminum is
0.17 mg/l Al.
4.
Al
Al2O
3
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
22
MC500 Colorimeter 06-2010
1.1 Methods
Ammonium
with Tablet
0.02 – 1 mg/l N
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one AMMONIA No. 1 tablet straight from the foil
to the water sample and crush the tablet using a clean
stirring rod.
6. Add one AMMONIA No. 2 tablet straight from the foil
to the same water sample and crush the tablet using a
clean stirring rod.
7. Close the vial tightly with the cap and swirl several times
until the tablets are dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
Wait for a reaction period of 10 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in mg/l Ammonium.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
10:00
6
0
23
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. The tablets must be added in the correct sequence.
2. The AMMONIA No. 1 tablet will only dissolve completely after the AMMONIA No. 2
tablet has been added.
3. The temperature of the sample is important for full color development.
At a temperature below 20°C the reaction period is 15 minutes.
4. Sea water samples:
Ammonia conditioning reagent is required when testing sea water or brackish water
samples to prevent precipitations of salts.
Fill the test tube with the sample to the 10 ml mark and add one level spoonful of
Conditioning Powder. Mix to dissolve, then continue as described in the test instructions.
5. Conversion:
mg/l NH4 = mg/l N x 1.29
mg/l NH3 = mg/l N x 1.22
6.
N
NH
4
NH
3
24
MC500 Colorimeter 06-2010
1.1 Methods
Ammonia
with Powder Pack
0.01 – 0.8 mg/l N
Use two clean vials (24 mm Ø) and mark one as blank for
zeroing.
1. Fill a clean vial (24 mm Ø) with 10 ml of deionized water
(this is the blank).
2. Fill the other clean vial (24 mm Ø) with 10 ml of the water
sample (this is the sample).
3. Add the contents of one Ammonia Salicylate F10
Powder Pack straight from the foil to each vial.
4. Close the vials with the caps and shake to mix the contents.
5. Press [
] key.
Wait for a reaction period of 3 minutes.
After the reaction period is nished proceed as follows:
6. Add the contents of one Ammonia Cyanurate F10
Powder Pack straight from the foil to each sample.
7. Close the vials tightly with the caps and shake to mix the
contents.
8. Press [
] key.
Wait for a reaction period of 15 minutes.
After the reaction period is nished proceed as follows:
9. Place the vial (the blank) in the sample chamber making
sure that the marks are aligned.
10. Press ZERO key.
11. Remove the vial from the sample chamber.
12. Place the vial (the sample) in the sample chamber
making sure that the
marks are aligned.
13. Press TEST key.
The result is shown in the display in mg/l Ammonium.
1 2
3 4
Countdown 1
3:00
start:
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown 2
15:00
start:
6
2
25
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Extremely basic or acidic water samples should be adjusted with 0.5 mol/l (1 N) Sulfuric
acid solution or 1 mol/l (1 N) Sodium hydroxide solution to pH 7.
2. Interferences:
Interfering substance Interference levels and treatments
Calcium greater than 1000 mg/l CaCO
3
Iron Interferes at all levels. Correct as follows:
a) determine the concentration of iron present in the
sample by performing a total Iron test
b) add the same iron concentration as determined
to the deionized water (step 1).
The interference will be blanked out successfully.
Magnesium greater than 6000 mg/l CaCO
3
Nitrate greater than 100 mg/l NO3-N
Nitrite greater than 12 mg/l NO2-N
Phosphate greater than 100 mg/l PO4-P
Sulfate greater than 300 mg/l SO
4
Sulde intensies the color
Glycine, Hydrazine,
Color, Turbidity
Less common interferences such as Hydrazine and
Glycine will cause intensied colors in the prepared
sample. Turbidity and color will give erroneous high
values. Samples with severe interferences require
distillation.
3.
N
NH
4
NH
3
26
MC500 Colorimeter 06-2010
1.1 Methods
Ammonia LR
with Vario Tube Test
0.02 – 2.5 mg/l N
Insert the adapter for 16 mm Ø vials.
1. Open one white capped reaction vial and add 2 ml
deionized water (this is the blank).
2. Open another white capped reaction vial and add
2 ml of the water sample (this is the sample).
3. Add the contents of one AMMONIA Salicylate
F5 Powder Pack straight from the foil into each vial.
4. Add the contents of one AMMONIA Cyanurate
F5 Powder Pack straight from the foil into each vial.
5. Close the vials tightly with the caps and swirl several
times to dissolve the powder.
6. Press [
] key.
Wait for a reaction period of 20 minutes.
After the reaction period is nished proceed as follows:
7. Place the vial (the blank) in the sample chamber making
sure that the marks are l aligned.
8. Press ZERO key.
9. Remove the vial from the sample chamber.
10. Place the vial (the sample) in the sample chamber
making sure that the marks are
l
aligned.
11. Press TEST key.
The result is shown in the display in mg/l Ammonium.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown 1
20:00
start:
1 2
3 4
Ø 16 mm
6
5
27
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Strong alkaline or acidic water samples must be adjusted to approx. pH 7 before analysis
(use 1 mol/l Hydrochloric acid resp. 1 mol/l Sodium hydroxide).
2. If chlorine is known to be present, add one drop of 0.1 mol/l Sodium thiosulfate for
each 0.3 mg/l Cl2 in a one liter (1000 mL) water sample.
3. Iron interferes with the test. The interferences will be eliminated as follows:
Determine the amount of total iron present in the water sample. To produce the blank
add an iron standard solution with the same iron concentration to the vial (point 1)
instead of deionized water
4. Conversion:
mg/l NH4 = mg/l N x 1.29
mg/l NH3 = mg/l N x 1.22
5.
N
NH
4
NH
3
28
MC500 Colorimeter 06-2010
1.1 Methods
Ammonia HR
with Vario Tube Test
1 – 50 mg/l N
Insert the adapter for 16 mm Ø vials.
1. Open one white capped reaction vial and add 0.1 ml
deionized water (this is the blank).
2. Open another white capped reaction vial and add
0.1 ml of the water sample (this is the sample).
3. Add the contents of one Vario AMMONIA Salicylate
F5 Powder Pack straight from the foil into each vial.
4. Add the contents of one Vario AMMONIA Cyanurate
F5 Powder Pack straight from the foil into each vial.
5. Close the vials tightly with the caps and swirl several
times to dissolve the powder.
6. Press [
] key.
Wait for a reaction period of 20 minutes.
After the reaction period is nished proceed as follows:
7. Place the vial (the blank) in the sample chamber making
sure that the marks are l aligned.
8. Press ZERO key.
9. Remove the vial from the sample chamber.
10. Place the vial (the sample) in the sample chamber
making sure that the marks are
l
aligned.
11. Press TEST key.
The result is shown in the display
in mg/l
Ammonium.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown 1
20:00
start:
1 2
3 4
Ø 16 mm
6
6
29
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Strong alkaline or acidic water samples must be adjusted to approx. pH 7 before analysis
(use 1 mol/l Hydrochloric acid resp. 1 mol/l Sodium hydroxide).
2. If chlorine is known to be present, add one drop of 0.1 mol/l Sodium thiosulfate for each
0.3 mg/l Cl2 in a one litre water sample.
3. Iron interferes with the test. The interferences will be eliminated as follows:
Determine the amount of total iron present in the water sample. Add an iron standard
solution with the same concentration to the vial (point 1) instead of deionized water to
produce the blank.
4. Conversion:
mg/l NH4 = mg/l N x 1.29
mg/l NH3 = mg/l N x 1.22
5.
N
NH
4
NH
3
30
MC500 Colorimeter 06-2010
1.1 Methods
Boron
with Tablet
0.1 – 2 mg/l B
1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,
close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one BORON No. 1 tablet straight from the foil to the
water sample and crush the tablet using a clean stirring
rod and dissolve the tablet.
6. Add one BORON No. 2 tablet straight from the foil to
the same water sample and crush the tablet using a clean
stirring rod.
7. Close the vial tightly with the cap and swirl several times
until the tablets are dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
Wait for a reaction period of 20 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in mg/l Boron.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
20:00
8
5
31
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. The tablets must added in the correct sequence.
2. The sample solution should have a pH value between 6 and 7.
3. Interferences are prevented by the presence of EDTA in the tablets.
4. The rate of color development depends on the temperature. The temperature of the
sample must be 20°C ± 1°C.
5.
B
H3BO
3
32
MC500 Colorimeter 06-2010
1.1 Methods
Bromine
with Tablet
0.05 – 13 mg/l Br
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty it,
leaving a few drops remaining in the vial.
5. Add one DPD No. 1 tablet straight from the foil and
crush the tablet using a clean stirring rod.
6. Add water sample to the 10 ml mark.
7. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
The result is shown in the display in mg/l Bromine.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
8
0
33
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Vial cleaning:
As many household cleaners (e.g. dishwasher detergent) contain reducing substances,
the subsequent determination of Bromine may show lower results. To avoid any
measurement errors, only use glassware free of Chlorine consumption.
Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for
one hour, then rinse all glassware thoroughly with deionized water.
2. Preparing the sample:
When preparing the sample, the lost of Bromine, e.g. by pipetting or shaking,
must be avoided. The analysis must take place immediately after taking the sample.
3. The DPD color development is carried out at a pH value of 6.3 to 6.5. The reagent
tablet therefore contains a buer for the pH adjustment. Strong alkaline or acidic water
samples must be adjusted between pH 6 and pH 7 before the reagent is added
(use 0.5 mol/l Sulfuric acid resp. 1 mol/l Sodium hydroxide).
4. Exceeding of the measuring range:
Concentrations above 22 mg/l Bromine can produce results within the measuring range
up to 0 mg/l. In this case, the water sample must be diluted with water free of
Bromine. 10 ml of the diluted sample will be mixed with the reagent and the
measurement repeated.
Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Bromine.
34
MC500 Colorimeter 06-2010
1.1 Methods
Chloramine (Mono) and Free Ammonia
with Powder Pack and Liquid Reagent
0.04 – 4.50 mg/l Cl
2
The following selection is shown in the display:
for the determination of Monochloramine and free
Ammonia
for the determination of Monochloramine
Select the desired determination with the arrow keys [
]
and [ ]. Confirm with [ ] key.
Notes:
1. Full color development – temperature
The reaction periods indicated in the manual refer to a sample temperature between
64.4° and 68°F (18° and 20°C). Due to the fact that the reaction period is strongly
influenced by sample temperature, you have to adjust both reaction periods according to
the following table:
Sample temperature in
°F / °C
Reaction period in min
41 / 5 10
50 / 10 8
60.8 / 16 6
68 / 20 5
73.4 / 23 2.5
77 / 25 2
6 3
Chloramine (Mono)
>> with NH4
without NH4
>> with NH4
>> without NH4
35
MC500 Colorimeter 06-2010
1.1 Methods
Chloramine (Mono)
with Powder Pack
0.04 – 4.50 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. A d d the contents of one Mo n o chlor F RGT
Powder Pack straight from the foil into the water
sample.
6. Close the vial tightly with the cap and swirl several times
to mix the contents (approx. 20 seconds).
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
Wait for a reaction period of 5 minutes.
After the reaction period is finished the measurement
starts automatically.
The result is shown in the display in mg/l
Monochloramine.
mg/l Cl
2
mg/l NH2Cl
mg/l N
Notes:
see previous page
6
3
1 2
3 4
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
5:00
36
MC500 Colorimeter 06-2010
1.1 Methods
Chloramine (Mono) and Free Ammonia
with Powder Pack and Liquid Reagent
0.04 – 4.50 mg/l Cl
2
0.01 – 0.50 mg/l NH4-N
Use two clean vials (24 mm Ø) and mark one as the
chloramine vial, the other as the ammonia vial.
1. Fill both vials (24 mm Ø) with 10 ml of water sample,
close tightly with the cap.
2. Place the chloramine vial in the sample chamber making
sure that the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add the contents of one Monochlor F RGT Powder
Pack straight from the foil into the chloramine vial.
6. Close the vial tightly with the cap and swirl several times
to mix the contents (approx. 20 seconds).
7. Add 1 drop of Free Ammonia Reagent Solution into
the ammonia vial (Note 1).
8. Close the vial tightly with the cap and invert several
times to mix the contents.
9. Place the chloramine vial in the sample chamber making
sure that the
marks are aligned.
10. Press TEST key.
Wait for a reaction period of 5 minutes.
After the reaction period is finished the measurement
starts automatically.
11. Remove the vial from the sample chamber.
12. Add the contents of one Monochlor F RGT Powder
Pack straight from the foil into the ammonia vial.
1 2
3 4
6 3
prepare Zero
press ZERO
Zero accepted
prepare T1
press TEST
Countdown
5:00
37
MC500 Colorimeter 06-2010
1.1 Methods
13. Close the vial tightly with the cap and swirl several times
to mix the contents (approx. 20 seconds).
14. Place the ammonia vial in the sample chamber making
sure that the
marks are aligned.
15. Press TEST key.
Wait for a reaction period of 5 minutes.
After the reaction period is finished the measurement
starts automatically.
The result is shown in the display in mg/l
Monochloramine and mg/l free Ammonia.
The reading of Monochloramine is shown as:
mg/l Cl
2
mg/l NH2Cl
mg/l N
The reading of Ammonia is shown as N.
Conversion:
mg/l NH
4
= mg/l N · 1.29
mg/l NH3 = mg/l N · 1.22
Notes:
1. Hold the bottle vertically and squeeze slowly.
2. To determine the ammonia concentration the difference between the chloramine (T1)
and the sum of chloramine and ammonia (T2) is calculated. If T2 exceeds the range limit
the following message is displayed:
NH
2
Cl + NH4 > 0.5 mg/l
In this case the sample has to be diluted and the measurement repeated.
3. see also page 34
T1 accepted
prepare T2
press TEST
Countdown
5:00
*,** mg/l N [NH4]
*,** mg/l Cl2
*,** mg/l NH2Cl
*,** mg/l N [NH2Cl]
38
MC500 Colorimeter 06-2010
1.1 Methods
Chloride
with Tablet
0.5 – 25 mg/l Cl
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one CHLORIDE T1 tablet straight from the foil
to the water sample, crush the tablet using a clean
stirring rod and dissolve the tablet.
6. Add one CHLORIDE T2 tablet straight from the foil
to the same water sample and crush the tablet using a
clean stirring rod.
7. Close the vial tightly with the cap and swirl gently
several times until the tablet is dissolved (Note 1).
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
Wait for a reaction period of 2 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in mg/l Chloride.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
2:00
9
0
39
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Ensure that all particles of the tablet are dissolved – Chloride causes an extremely ne
distributed turbidity with a milky appearance.
Heavy shaking leads to bigger sized particles which can cause false readings.
2. High concentrations of electrolytes and organic compounds have dierent eects on the
precipitation reaction.
3. Ions which also form deposits with Silver nitrate in acidic media, such as Bromides,
Iodides and Thiocyanates, interfere with the analysis.
4. Highly alkaline water should - if necessary - be neutralized using Nitric acid before
analysis.
40
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine
with Tablet
0.01 – 6 mg/l Cl
2
Chlorine
with Liquid Reagent
0.02 – 4 mg/l Cl
2
Chlorine
with Powder Pack
0.02 – 2 mg/l Cl
2
The following selection is shown in the display:
for the dierentiated determination of free, combined and
total Chlorine.
for the determination of free Chlorine.
for the determination of total Chlorine.
Select the desired determination with the arrow
keys [
] and [ ]. Conrm with [ ] key.
Chlorine
>> di
free
total
>> di
>> free
>> total
0
0
1
0
1
1
0
1
1
41
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Vial cleaning:
As many household cleaners (e.g. dishwasher detergent) contain reducing substances,
the subsequent determination of Chlorine may show lower results. To avoid any
measurement errors, only use glassware free of Chlorine consumption.
Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for
one hour, then rinse all glassware thoroughly with deionized water.
2. For individual testing of free and total Chlorine, the use of dierent sets of glassware is
recommend (EN ISO 7393-2, 5.3)
3. Preparing the sample:
When preparing the sample, the lost of Chlorine, e.g. by pipetting or shaking,
must be avoided. The analysis must take place immediately after taking the sample.
4. The DPD color development is carried out at a pH value of 6.3 to 6.5. The reagents
therefore contain a buer for the pH adjustment.
Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7
before the reagent is added (use 0.5 mol/l Sulfuric acid resp. 1 mol/l Sodium hydroxide).
5. Exceeding of the measuring range:
Concentrations above
10 mg/l Chlorine using tablets
4 mg/l Chlorine using liquid reagents
2 mg/l using powder packs
can produce results within the measuring range up to 0 mg/l. In this case, the water
sample must be diluted with water free of Chlorine. 10 ml of the diluted sample will be
mixed with the reagent and the measurement repeated.
6. Turbidity (lead to errors):
The use of the DPD No. 1 tablet (method 100) in samples with high Calcium ion
content* and/or high conductivity* can lead to turbidity of the sample and therefore
incorrect measurements. In this case, the reagent tablet DPD No. 1 High Calcium
should be used as an alternative. Even if the turbidity does occur after the DPD No. 3
tablet has been added, this can be prevented by using the DPD No. 1 HIGH CALCIUM
tablet.
* it is not possible to give exactly values, because the development of turbidity depends
on nature and ingredients of the sample.
7. If ??? is displayed at a dierentiated test result see page 260.
Oxidizing agents such as Bromine, Ozone etc. interfere as they react like Chlorine.
42
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, dierentiated determination
with Tablet
0.01 – 6 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty it,
leaving a few drops remaining in the vial.
5. Add one DPD No. 1 tablet straight from the foil and
crush the tablet using a clean stirring rod.
6. Add water sample to the 10 ml mark.
7. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
10. Remove the vial from the sample chamber.
11. Add one DPD No. 3 tablet straight from the foil to the
same water sample and crush the tablet using a clean
stirring rod.
12. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
prepare Zero
press ZERO
Zero accepted
prepare T1
press TEST
0
01
43
MC500 Colorimeter 06-2010
1.1 Methods
13. Place the vial in the sample chamber making sure that
the marks are aligned.
14. Press TEST key.
Wait for a reaction period of 2 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in:
mg/l free Chlorine
mg/l combined Chlorine
mg/l total Chlorine
Notes:
See page 41.
T1 accepted
prepare T2
press TEST
Countdown
2:00
*,** mg/l free Cl
*,** mg/l comb Cl
*,** mg/l total Cl
44
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, free
with Tablet
0.01 – 6 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty it,
leaving a few drops remaining in the vial.
5. Add one DPD No. 1 tablet straight from the foil and
crush the tablet using a clean stirring rod.
6. Add water sample to the 10 ml mark.
7. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
The result is shown in the display in mg/l
free Chlorine.
Notes:
See page 41.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
0
01
45
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, total
with Tablet
0.01 – 6 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty it,
leaving a few drops remaining in the vial.
5. Add one DPD No. 1 tablet and one DPD No. 3 tablet
straight from the foil and crush the tablets using a clean
stirring rod.
6. Add water sample to the 10 ml mark.
7. Close the vial tightly with the cap and swirl several times
until the tablets are dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
Wait for a reaction period of 2 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in mg/l total
Chlorine.
Notes:
See page 41.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
2:00
0
01
46
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, dierentiated determination
with Liquid Reagent
0.02 – 4 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty
the vial.
5. Fill the vial with drops of the same size by holding the
bottle vertically and squeeze slowly:
6 drops of DPD 1 buer solution
2 drops of DPD 1 reagent solution
6. Add water sample to the 10 ml mark.
7. Close the vial tightly with the cap and swirl several times
to mix the contents.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
10. Remove the vial from the sample chamber.
11. Add 3 drops of DPD 3 solution to the same water
sample.
12. Close the vial tightly with the cap and swirl several times
to mix the contents.
prepare Zero
press ZERO
Zero accepted
prepare T1
press TEST
0
1
1
47
MC500 Colorimeter 06-2010
1.1 Methods
13. Place the vial in the sample chamber making sure that
the marks are aligned.
14. Press TEST key.
Wait for a reaction period of 2 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in:
mg/l free Chlorine
mg/l combined Chlorine
mg/l total Chlorine
Notes:
1. After use replace the bottle caps securely noting the color coding.
2. Store the reagent bottles in a cool, dry place ideally between 6°C and 10°C.
3. Also see page 41.
T1 accepted
prepare T2
press TEST
Countdown
2:00
*,** mg/l free Cl
*,** mg/l comb. Cl
*,** mg/l total Cl
48
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, free
with Liquid Reagent
0.02 – 4 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty
the vial.
5. Fill the vial with drops of the same size by holding the
bottle vertically and squeeze slowly:
6 drops of DPD 1 buer solution
2 drops of DPD 1 reagent solution
6. Add water sample to the 10 ml mark.
7. Close the vial tightly with the cap and swirl several times
to mix the contents.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
The result is shown in the display in mg/l
free Chlorine.
Notes (free and total Chlorine):
1. After use replace the bottle caps securely noting the color coding.
2. Store the reagent bottles in a cool, dry place ideally between 6°C and 10°C.
3. Also see page 41.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
0
1
1
49
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, total
with Liquid Reagent
0.02 – 4 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty
the vial.
5. Fill the vial with drops of the same size by holding the
bottle vertically and squeeze slowly:
6 drops of DPD 1 buer solution
2 drops of DPD 1 reagent solution
3 drops of DPD 3 solution
6. Add water sample to the 10 ml mark.
7. Close the vial tightly with the cap and swirl several times
to mix the contents.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
Wait for a reaction period of 2 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in mg/l total
Chlorine.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
2:00
0
1
1
50
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, dierentiated determination
with Powder Pack
0.02 – 2 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add the con tents o f one Chlorine FREE-DPD/
F10 Powder Pack straight from the foil to the water
sample.
6. Close the vial tightly with the cap and swirl several times
to mix the contents (approx. 20 seconds).
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
9. Remove the vial from the sample chamber, empty the
vial, rinse vial and cap several times and then ll the vial
with 10 ml of the water sample.
10. Add the contents of one Chlorine TOTAL-DPD /
F10 Powder Pack straight from the foil to the water
sample.
11. Close the vial tightly with the cap and swirl several times
to mix the contents (approx. 20 seconds).
prepare Zero
press ZERO
Zero accepted
prepare T1
press TEST
1 2
3 4
0
1
1
51
MC500 Colorimeter 06-2010
1.1 Methods
12. Place the vial in the sample chamber making sure that
the marks are aligned.
13. Press TEST key.
Wait for a reaction period of 3 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in:
mg/l free Chlorine
mg/l combined Chlorine
mg/l total Chlorine
Notes:
See page 41.
*,** mg/l free Cl
*,** mg/l comb. Cl
*,** mg/l total Cl
T1 accepted
prepare T2
press TEST
Countdown
3:00
52
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, free
with Powder Pack
0.02 – 2 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add the content s of one Chlorin e FREE-DPD /
F10 Powder Pack straight from the foil to the water
sample.
6. Close the vial tightly with the cap and swirl several times
to mix the contents (approx. 20 seconds).
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
The result is shown in the display in mg/l free
Chlorine.
Notes:
See page 41.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
1 2
3 4
0
1
1
53
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine, total
with Powder Pack
0.02 – 2 mg/l Cl
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add the contents of one Chlorine TOTAL-DPD /
F10 Powder Pack straight from the foil to the water
sample.
6. Close the vial tightly with the cap and swirl several times
to mix the contents (approx. 20 seconds).
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
Wait for a reaction period of 3 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in mg/l total
Chlorine.
Notes:
See page 41.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
1 2
3 4
Countdown
3:00
0
1
1
54
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine dioxide
with Tablet
0.05 – 11 mg/l ClO
2
The following selection is shown in the display:
for the determination of Chlorine dioxide in the presence
of Chlorine.
for the determination of Chlorine dioxide in the absence of
Chlorine.
Select the desired determination with the arrow keys
[
] and [ ]. Conrm with [ ] key.
Chlorine dioxide
>> with Cl
without Cl
>> with Cl
>> without Cl
2
0
1
55
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Vial cleaning:
As many household cleaners (e.g. dishwasher detergent) contain reducing substances,
the subsequent determination of Chlorine dioxide may show lower results. To avoid
any measurement errors, only use glassware free of Chlorine consumption.
Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for
one hour, then rinse all glassware thoroughly with deionized water.
2. Preparing the sample:
When preparing the sample, the lost of Chlorine dioxide, e.g. by pipetting or
shaking, must be avoided. The analysis must take place immediately after taking the
sample.
3. The DPD color development is carried out at a pH value of 6.3 to 6.5. The reagent
tablet therefore contains a buer for the pH adjustment.
Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7
before the tablet is added (use 0.5 mol/l Sulfuric acid resp. 1 mol/l Sodium hydroxide).
4. Exceeding of the measuring range:
Concentrations above 19 mg/l Chlorine dioxide can produce results within the
measuring range up to 0 mg/l. In this case, the water sample must be diluted with
water free of Chlorine dioxide. 10 ml of the diluted sample will be mixed with the
reagent and the measurement repeated.
5. If ??? is displayed at a dierentiated test result see page 260.
Oxidizing agents such as Chlorine, Ozone etc. interfere as they react like Chlorine dioxide.
56
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine dioxide
in the presence of Chlorine
with Tablet
0.05 – 11 mg/l ClO
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty it,
leaving a few drops remaining in the vial.
5. Add one DPD No. 1 tablet straight from the foil and
crush the tablet using a clean stirring rod.
6.
Fill a second clean vial with 10 ml of the water
sample.
7. Add one GLYCINE tablet straight from the foil and
crush the tablet using a clean stirring rod.
8. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
9. Transfer the contents of the second vial into the
prepared vial.
10. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
11. Place the vial in the sample chamber making sure that
the
marks are aligned.
12. Press TEST key.
Zero accepted
prepare T1
press TEST
prepare Zero
press ZERO
2
0
1
57
MC500 Colorimeter 06-2010
1.1 Methods
13. Remove the vial from the sample chamber, empty the
vial, rinse vial and cap several times. Fill with a few
drops of the water sample.
14. Add one DPD No. 1 tablet straight from the foil and
crush the tablet using a clean stirring rod.
15. Add water sample to the 10 ml mark.
16. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
17. Place the vial in the sample chamber making sure that
the
marks are aligned.
18. Press TEST key.
19. Remove the vial from the sample chamber.
20. Add one DPD No. 3 tablet straight from the foil to the
same water sample and crush the tablet using a clean
stirring rod.
21. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
22. Place the vial in the sample chamber making sure that
the
marks are aligned.
23. Press TEST key.
Wait for a reaction period of 2 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in:
as Chlorine dioxide in mg/l Chlorine,
or
as Chlorine dioxide in mg/l ClO2.
mg/l free Chlorine
mg/l combined Chlorine
mg/l total Chlorine
Notes:
See next page.
T1 accepted
prepare T2
press TEST
Countdown
2:00
T2 accepted
prepare T3
press TEST
*,** mg/l free Cl
*,** mg/l comb. Cl
*,** mg/l total Cl
*,** mg/l ClO2 [Cl]
*,** mg/l ClO2
58
MC500 Colorimeter 06-2010
1.1 Methods
Notes: (Chlorine dioxide in the presence of Chlorine)
1. The conversion factor to convert Chlorine dioxide as Chlorine to Chlorine dioxide as ClO2
is approximately 0.4 (more exactly 0.38).
mg/l ClO2 = mg/l ClO2 [Cl] x 0.38
ClO2[Cl]
ClO
2
(Chlorine dioxide displayed as Chlorine units ClO2 [Cl] has its origin out of the swimming
poolwater treatment according to DIN 19643.)
2. The total Chlorine result given includes the contribution by the Chlorine dioxide (as
Chlorine) reading. For true total Chlorine value subtract the Chlorine dioxide (as
Chlorine) reading from the quoted total Chlorine reading.
3. Also see page 55.
59
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine dioxide
in absence of Chlorine
with Tablet
0.05 – 11 mg/l ClO
2
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty it,
leaving a few drops remaining in the vial.
5. Add one DPD No. 1 tablet straight from the foil and
crush the tablet using a clean stirring rod.
6. Add water sample to the 10 ml mark.
7. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
The result is shown in the display
as Chlorine dioxide in mg/l Chlorine,
or
as Chlorine dioxide in mg/l ClO
2
.
Notes:
See page 55.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
*,** mg/l ClO2
*,** mg/l ClO2 [Cl]
2
0
1
60
MC500 Colorimeter 06-2010
1.1 Methods
Chlorine HR (Kl)
with Tablet
5 – 200 mg/l Cl
2
Insert the adapter for 16 mm Ø vials.
1. Fill a clean vial (16 mm Ø) with 8 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the marks are
l
aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one CHLORINE HR (KI) tablet straight from
the foil to the water sample and crush the tablet using
a clean stirring rod.
6. Add one ACIDIFYING GP tablet straight from the
foil to the same water sample and crush the tablet using a
clean stirring rod.
7. Close the vial tightly with the cap and swirl several times
until the tablets are dissolved.
8. Place the vial in the sample chamber making sure
that the marks are
l
aligned.
9. Press TEST key.
The result is shown in the display in mg/l Chlorine.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Ø 16 mm
0
5
1
61
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Oxidizing agents interfere as they react like Chlorine.
62
MC500 Colorimeter 06-2010
1.1 Methods
COD LR
with Vario Tube Test
0 – 150 mg/l O
2
Insert the adapter for 16 mm Ø vials.
1. Open one white capped reaction vial and add 2 ml
deionized water (this is the blank (Note 1)).
2. Open another white capped reaction vial and add
2 ml of the water sample (this is the sample).
3. Close the vials with the cap tightly. Invert the vial gently
several times to mix the contents.
(CAUTION: The vial will become hot during mixing!)
4. Heat the vials for 120 minutes in the preheated reactor
at a temperature of 150°C.
5. (CAUTION: The vials are hot!)
Remove the tubes from the heating block and allow them
to cool to 60°C or less. Mix the contents by carefully
inverting each tube several times while still warm.
Then allow the tubes to cool to ambient temperature
before measuring. (Note 2).
6. Place the vial (the blank (Note 3, 4)) in the sample
chamber making sure that the marks are
l
aligned.
7. Press ZERO key.
8. Remove the vial from the sample chamber.
9. Place the vial (the sample (Note 3, 4)) in the sample
chamber making sure that the marks are
l
aligned.
10. Press TEST key.
The result is shown in the display in mg/l COD.
Zero accepted
prepare Test
press TEST
prepare Zero
press ZERO
Ø 16 mm
3
0
1
63
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Run samples and blanks with the same batch of vials. The blank is stable when stored in
the dark and can be used for further measurements with vials of the same batch.
2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for
nal measurements.
3. Suspended solids in the vial lead to incorrect measurements. For this reason it is
important to place the vials carefully in the sample chamber. The precipitate at the
bottom of the sample should be not suspended.
4.
Clean the outside of the vials with a towel to remove nger prints or other marks.
5. Samples can be measured when the Chloride contents does not exceed 1000 mg/l.
6. Be aware that low readings can result if the water contains compounds that cannot be
oxidized completely, as compared to the reference method.
64
MC500 Colorimeter 06-2010
1.1 Methods
COD MR
with Vario Tube Test
0 – 1500 mg/l O
2
Insert the adapter for 16 mm Ø vials.
1. Open one white capped reaction vial and add 2 ml
deionized water (this is the blank (Note 1)).
2. Open another white capped reaction vial and add
2 ml of the water sample (this is the sample).
3. Close the vials with the cap tightly. Invert the vial gently
several times to mix the contents.
(CAUTION: The vial will become hot during mixing!)
4. Heat the vials for 120 minutes in the preheated reactor
at a temperature of 150°C.
5. (CAUTION: The vials are hot!)
Remove the tubes from the heating block and allow them
to cool to 60°C or less. Mix the contents by carefully
inverting each tube several times while still warm.
Then allow the tubes to cool to ambient temperature
before measuring. (Note 2).
6. Place the vial (the blank (Note 3, 4)) in the sample
chamber making sure that the marks are
l
aligned.
7. Press ZERO key.
8. Remove the vial from the sample chamber.
9. Place the vial (the sample (Note 3, 4)) in the sample
chamber making sure that the marks are
l
aligned.
10. Press TEST key.
The result is shown in the display in mg/l COD.
Zero accepted
prepare Test
press TEST
prepare Zero
press ZERO
Ø 16 mm
3
1
1
65
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Run samples and blanks with the same batch of vials. The blank is stable when stored in
the dark and can be used for further measurements with vials of the same batch.
2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for
nal measurements.
3. Suspended solids in the vial lead to incorrect measurements. For this reason it is
important to place the vials carefully in the sample chamber. The precipitate at the
bottom of the sample should be not suspended.
4.
Clean the outside of the vials with a towel to remove nger prints or other marks.
5. Samples can be measured when the Chloride contents does not exceed 1000 mg/l.
6. Be aware that low readings can result if the water contains compounds that cannot be
oxidized completely, as compared to the reference method.
7. For samples under 100 mg/l COD it is recommended to repeat the test with the tube
test for COD LR.
66
MC500 Colorimeter 06-2010
1.1 Methods
COD HR
with Vario Tube Test
0 – 15 g/l O2 (=^ 0 – 15 ,000 mg/l O2)
Insert the adapter for 16 mm Ø vials.
1. Open one white capped reaction vial and add 0.2 ml
deionized water (this is the blank (Note 1)).
2. Open another white capped reaction vial and add
0.2 ml of the water sample (this is the sample).
3. Close the vials with the cap tightly. Invert the vial gently
several times to mix the contents.
(CAUTION: The vial will become hot during mixing!)
4. Heat the vials for 120 minutes in the preheated reactor
at a temperature of 150°C.
5. (CAUTION: The vials are hot!)
Remove the tubes from the heating block and allow them
to cool to 60°C or less. Mix the contents by carefully
inverting each tube several times while still warm.
Then allow the tubes to cool to ambient temperature
before measuring. (Note 2).
6. Place the vial (the blank (Note 3, 4)) in the sample
chamber making sure that the marks are
l
aligned.
7. Press ZERO key.
8. Remove the vial from the sample chamber.
9. Place the vial (the sample (Note 3, 4)) in the sample
chamber making sure that the marks are
l
aligned.
10. Press TEST key.
The result is shown in the display in g/l COD.
Zero accepted
prepare Test
press TEST
prepare Zero
press ZERO
Ø 16 mm
2
31
67
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Run samples and blanks with the same batch of vials. The blank is stable when stored in
the dark and can be used for further measurements with vials of the same batch.
2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for
nal measurements.
3. Suspended solids in the vial lead to incorrect measurements. For this reason it is
important to place the vials carefully in the sample chamber. The precipitate at the
bottom of the sample should be not suspended.
4.
Clean the outside of the vials with a towel to remove nger prints or other marks.
5. Samples can be measured when the Chloride contents does not exceed 10 000 mg/l.
6. Be aware that low readings can result if the water contains compounds that cannot be
oxidized completely, as compared to the reference method.
7. For samples under 1 g/l COD it is recommended to repeat the test with the test kit for
COD MR or for samples under 0,1 g/l COD with the tube test COD LR.
68
MC500 Colorimeter 06-2010
1.1 Methods
Color, true and apparent
(APHA Platinum-Cobalt
Standard Method)
0 – 500 Pt-Co units (mg/l Pt)
Sample preparation (Note 4):
Step A
Filter approx. 50 ml deionized water through a membrane
filter with a pore width of 0.45 µm.
Discard the filtrate. Filter another 50 ml deionized water
and keep it for zeroing.
Step B
Filter approx. 50 ml water sample using the same filter. Keep
this filtrate for sample measurement.
1. Fill a clean vial (24 mm Ø) with 10 ml of the filtrated
deionized water (from Step A), close tightly with the
cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber and empty
it completely.
5. Rinse the vial with the filtrated water sample and fill
with 10 ml ltrated water sample (from Step B).
6. Place the vial in the sample chamber making sure that
the
marks are aligned.
7. Press TEST key.
The result is shown in the display in Pt-Co units (mg/l Pt).
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
2
0 4
69
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. This color scale was originally developed by A. Hazen as a visual comparison scale.
It is therefore necessary to ascertain whether the extinction maximum of the water
sample is in the range from 420 to 470 nm, as this method is only suitable for water
samples with yellowish to yellowish-brown coloration. Where applicable, a decision
should be made based on visual inspection of the water sample.
2. This method 204 – Hazen – is calibrated on the basis of the standards specified by
”Standard Methods for the Examination of Water and Wastewater” (also see EN ISO
7887:1994).
1 Pt-Co color unit = 1 mg/L of platinum as chloroplatinate ion
3. The estimated detection limit is 15 mg/L Pt.
4. Color may be expressed as “apparent” or “true” color. The apparent color is defined
as the color of a solution due to dissolved substances and suspended particles in the
sample. This manual describes the determination of true color by filtration of the water
sample. To determine the apparent color, non-filtrated deionized water and sample are
measured.
5. Sample collection, preservation and storage:
Pour the water sample into clean glass or plastic containers and analyse as soon as
possible after the sample is taken. If this is not possible, fill the container right up to the
top and seal tightly. Do not stir the sample; avoid lengthy contact with the air.
The sample may be stored in a dark place at a temperature of 39.2°F (4°C) for 24 hours.
Before performing measurements, the water sample must be brought up to room
temperature.
70
MC500 Colorimeter 06-2010
1.1 Methods
Copper
with Tablet
0.05 – 5 mg/l Cu
The following selection is shown in the display:
for the dierentiated determination of free, combined and
total Copper.
for the determination of free Copper.
for the determination of total Copper.
Select the desired determination with the arrow keys
[
] and [ ]. Conrm with [ ] key.
Note:
1. If ??? is displayed at the dientiated test result see page 260.
Copper
>> di
free
total
>> di
>> free
>> total
5
0
1
71
MC500 Colorimeter 06-2010
1.1 Methods
Copper, dierentiated determination
with Tablet
0.05 – 5 mg/l Cu
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one COPPER No. 1 tablet straight from the foil
to the water sample and crush the tablet using a clean
stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
9. Remove the vial from the sample chamber.
10. Add one COPPER No. 2 tablet straight from the foil
to the same water sample and crush the tablet using a
clean stirring rod.
11. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
12. Place the vial in the sample chamber making sure that
the
marks are aligned.
13. Press TEST key.
The result is shown in the display in:
mg/l free Copper
mg/l combined Copper
mg/l total Copper
*,** mg/l free Cu
*,** mg/l comb Cu
*,** mg/l total Cu
Zero accepted
prepare T1
press TEST
prepare Zero
press ZERO
T1 accepted
prepare T2
press TEST
5
0
1
72
MC500 Colorimeter 06-2010
1.1 Methods
Copper, free
with Tablet
0.05 – 5 mg/l Cu
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one COPPER No. 1 tablet straight from the foil
to the water sample and crush the tablet using a clean
stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
The result is shown in the display in mg/l free Copper.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
5
0
1
73
MC500 Colorimeter 06-2010
1.1 Methods
Copper, total
with Tablet
0.05 – 5 mg/l Cu
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one COPPER No. 1 tablet and one COPPER
No. 2 tablet straight from the foil to the water sample
and crush the tablets using a clean stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablets are dissolved.
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
The result is shown in the display in mg/l total Copper.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
5
0
1
74
MC500 Colorimeter 06-2010
1.1 Methods
Copper, free (Note 1)
with Powder Pack
0.05 – 5 mg/l Cu
1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,
close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add the contents of one Cu 1 F10 Powder Pack straight
from the foil to the water sample.
6. Close the vial tightly with the cap and swirl several times
to mix the contents (Note 3).
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
Wait for a reaction period of 2 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in mg/l Copper
Countdown
2:00
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
1 2
3 4
5
3
1
75
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. For determination of Total Copper, a digestion is required.
2. Extremely acid water samples (pH 2 or less) must be adjusted between pH 4 and
pH 6 before the reagent is added (with 8 mol/l Potassium hydroxide solution KOH).
Caution: pH values above 6 can lead to Copper precipitation.
3. Accuracy is not aected by undissolved powder.
4. Interferences:
Cyanide, CN-Cyanide prevents full color development.
Add 0.2 ml Formaldehyde to 10 ml water sample and wait for a reaction
time of 4 minutes (Cyanide is masked). After this perform test as
described. Multiply the result by 1.02 to correct the sample dilution by
Formaldehyde.
Silver, Ag
+
If a turbidity remains and turns black, silver interferences is likely.
Add 10 drops of saturated Potassium chloride solution to 75 ml of water
sample. Filtrate through a ne lter. Use 10 ml of the ltered water
sample to perform test.
76
MC500 Colorimeter 06-2010
1.1 Methods
Cyanide
with Reagent Test
0.01 – 0.5 mg/l CN
1. Fill a clean vial (24 mm Ø) with 2 ml of the water sam-
ple and 8 ml of deionized water, close tightly with the
cap.
2. Place the vial in the sample chamber making sure that
the marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add two level spoons No. 4 (white) of Cyanide-11 into
the prepared water sample, replace the cap tightly and
invert the vial several times to mix the contents.
6. Add two level spoons No. 4 (white) of Cyanide-12,
replace the cap tightly and invert the vial several times
to mix the contents.
7. Fill the vial with drops of the same size by holding the
bottle vertically and squeeze slowly:
3 drops of Cyanide-13
8. Close the vial tightly with the cap and invert several times
to mix the contents.
9. Place the vial in the sample chamber making sure that
the
marks are aligned.
10. Press TEST key.
Wait for a reaction period of 10 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display in mg/l Cyanide.
Countdown
10:00
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
5
7
1
77
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Only free Cyanide and Cyanides that can be destroyed by Chlorine are determined by
this test.
2. In the present of Thiocyanate, heavy metal complexes, colorants or aromatic amines, the
cyanide must be separated out by distillation before analysis is performed.
3. Store the reagents in closed containers at a temperature of + 15°C to + 25°C.
78
MC500 Colorimeter 06-2010
1.1 Methods
Cyanuric acid
with Tablet
2 – 160 mg/l Cys
1. Fill a clean vial (24 mm Ø) with 5 ml of the water
sample and 5 ml deionized water (Note 1), close
tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one CYANURIC ACID tablet straight from the
foil to the prepared water sample and crush the tab let using a clean stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved (Note 2, 3).
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
The result is shown in the display in mg/l Cyanuric acid.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
6
0
1
79
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Use deionized water or tap water free of Cyanuric acid.
2. If Cyanuric acid is present a cloudy solution will result.
3. Dissolve the tablet completely (therefore swirl the vial approx. 1 minute).
Undissolved particles of the tablet can cause too high results.
80
MC500 Colorimeter 06-2010
1.1 Methods
DEHA (N,N-Diethylhydroxylamine)
with Tablet and Liquid Reagent
20 – 500 μg/l DEHA / 0.02 – 0.5 mg/l DEHA
1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample,
close tightly with the cap (Note 2).
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Fill the vial with drops of the same size by holding the
bottle vertically and squeeze slowly:
6 drops (0.25ml) of DEHA solution
6. Close the vial tightly with the cap and swirl several times
to mix the contents.
7. Add one DEHA tablet straight from the foil to the same
water sample and crush the tablet using a clean stirring
rod.
8. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
9. Place the vial in the sample chamber making sure that
the
marks are aligned.
10. Press TEST key.
Wait for a reaction period of 10 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display as DEHA.
Countdown
10:00
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
6
5
1
81
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Application: Testing of residual corrosion inhibitors (Oxygen scavengers) in boiler feed
water or condensate.
2. Before using clean the vials with Hydrochloric acid (approx. 20%). Rinse thoroughly with
deionized water.
3. Keep the sample dark during color development time. UV-light (sunlight) causes high
measurement results.
4. Ideal temperature for full color development is 20°C ± 2°C.
5. Interferences:
• Iron (II) interferes at all concentrations:
Repeat the test procedure but without adding the DEHA solution. If the
displayed result is above 20 μg/l subtract this value from the DEHA test result.
• Substances which reduce Iron (III) or complex iron may interfere.
• Substances which may interfere when present in concentrations at:
Borate (as Na2B4O7) 500 mg/l
Cobalt 0.025 mg/l
Copper 8.0 mg/l
Hardness (as CaCO
3
) 1000 mg/l
Lignosulfonates 0.05 mg/l
Manganese 0.8 mg/l
Molybdenum 80 mg/l
Nickel 0.8 mg/l
Phosphate 10 mg/l
Phosphonates 10 mg/l
Sulfate 1000 mg/l
Zinc 50 mg/l
6. There is an option to change the unit from mg/l to μg/l.
mg/l
µg/l
82
MC500 Colorimeter 06-2010
1.1 Methods
DEHA (N,N-Diethylhydroxylamine)
with Powder Pack
and Liquid Reagent
20 – 500 μg/l DEHA / 0.02 – 0.5 mg/l DEHA
Use two clean vials (24 mm Ø) and mark one as blank for
zeroing (Note 2).
1. Fill a clean vial with 10 ml deionized water (this is the
blank).
2. Fill the second clean vial with 10 ml of the water sample
(this is the sample).
3. Add
the contents of
one OXYSCAV 1 Rgt Powder Pack
straight from the foil into each vial.
4. Close the vials tightly with the caps and swirl several times
to mix the contents.
5. Add 0.20 ml DEHA 2 Rgt Solution to each vial (Note
4).
6. Close the vials tightly with the caps and swirl several times
to mix the contents.
7. Press [
] key.
Wait for a reaction period of 10 minutes (Note 5).
After the reaction period is nished proceed as follows:
8. Place the vial (the blank) in the sample chamber making
sure that the marks are aligned.
9. Press ZERO key.
10. Remove the vial from the sample chamber.
11. Place the vial (the sample) in the sample chamber making
sure that the
marks are aligned.
12. Press TEST key.
The result is shown in the display as DEHA.
Countdown 1
10:00
start:
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
1 2
3 4
6
7
1
83
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Application: Testing of residual corrosion inhibitors (Oxygen scavengers) in boiler feed
water or condensate.
2. Before using clean the vials with Hydrochloric acid (approx. 20%). Rinse thoroughly with
deionized water.
3. Ideally temperature for full color development is 25°C ± 3 °C.
4. Volume should always be metered by using suitable pipette (class A).
5. Keep blank and sample dark during color development time. UV-light (sunlight) causes
too high measurement results.
6. Interferences:
• Iron (II) interferes at all concentrations:
Repeat the test procedure but without adding the DEHA Rgt 2 solution. If the
displayed result is above 20 μg/l subtract this value from the DEHA test result.
• Substances which reduce Iron (III) interfere. Substances which complex iron strongly
may interfere also.
• Substances who may interfere when present in concentrations at:
Borate (as Na2B4O7) 500 mg/l
Cobalt 0.025 mg/l
Copper 8.0 mg/l
Hardness (as CaCO
3
) 1000 mg/l
Lignosulfonates 0.05 mg/l
Manganese 0.8 mg/l
Molybdenum 80 mg/l
Nickel 0.8 mg/l
Phosphate 10 mg/l
Phosphonates 10 mg/l
Sulfate 1000 mg/l
Zinc 50 mg/l
7. There is an option to change the unit from mg/l to μg/l.
mg/l
µg/l
84
MC500 Colorimeter 06-2010
1.1 Methods
Fluoride
with Liquid Reagent
0.05 – 2 mg/l F
Regard notes!
1. Fill a clean vial (24 mm Ø) with exactly 10 ml of water
sample (Note 4), close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add exactly 2 ml SPADNS reagent solution (Note 4)
to the water sample.
Caution: Vial is lled up to the top! (Note 8)
6. Close the vial tightly with the cap and swirl several times
to mix the contents.
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
Press TEST key.
The result is shown in the display in mg/l Fluoride.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
7
0
1
85
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. The same batch of SPADNS reagent solution must be used for adjustment and test.
The adjustment process needs to be performed for each new batch of SPADNS reagent
solution (see Standard Methods 20th, 1998, APHA, AWWA, WEF 4500 F D., S. 4-82).
The procedure is described in chapter 2.4.5 “Calibration" Mode 40“ on page 233.
2. During adjustment and test the same vial should be used for zeroing and test, as dierent
vials may exhibit minor tolerances.
3. The calibration solution and the water samples to be tested should have the same
temperature (± 1°C).
4. As the test result is highly dependent on exact sample and reagent volumes, the sample
and reagent volumes should always be metered by using a 10 ml resp. 2 ml volumetric
pipette (class A).
5. The accuracy of the test methods decreases above a level of 1.2 mg/l Fluoride. Although
the results are suciently accurate for most applications, even more exact results can be
achieved by 1:1 dilution of the sample prior to use and subsequent multiplication of the
result by 2.
6. SPADNS reagent solution contains Arsenite.
Chlorine concentrations up to 5 mg/l do not interfere.
7. Seawater and wastewater samples must be distilled.
8. It is convenient to use larger volume vials for this test.
86
MC500 Colorimeter 06-2010
1.1 Methods
Hardness, Calcium
with tablet reagent
50 – 900 mg/l CaCO
3
1. Fill a clean vial (24 mm Ø) with 10 ml deionized water.
2. Add one CALCHECK tablet straight from the foil to
the deionized water and crush the tablet using a clean
stirring rod.
3. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
4. Place the vial in the sample chamber making sure that
the
marks are aligned.
5. Press ZERO key.
Wait for a reaction period of 2 minutes.
After the reaction period is nished the measurement
starts automatically.
6. Remove the vial from the sample chamber.
7. Add 2 ml of the water sample to the prepared vial.
Caution: Vial is lled up to the top! (Note 4)
8. Close the vial tightly with the cap and swirl several times
(5x) to mix the contents.
9. Place the vial in the sample chamber making sure that
the
marks are aligned.
10. Press TEST key.
The result is shown in the display as Calcium Hardness.
Zero accepted
prepare Test
press TEST
prepare Zero
press ZERO
Countdown
2:00
9
0
1
87
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Strong alkaline or acidic water samples must be adjusted between pH 4 and pH 10
before the tablet is added (use 1 mol/l Hydrochloric acid resp. 1mol/l Sodium hydroxide).
2. The tolerance of the method is increased with higher concentrations. When diluting
samples, this should be taken into account, always measuring in the rst third of the range.
3. This method was developed from a volumetric procedure for the determination of
calcium. Due to undened conditions, the deviations from the standardized method may
be greater.
4. It is convenient to use larger volume vials for this test.
5.
CaCO
3
°dH
°eH
°fH
°aH
88
MC500 Colorimeter 06-2010
1.1 Methods
Hardness, Calcium 2T
with Tablet
0 – 500 mg/l CaCO
3
1. Fill a clean vial (24 mm Ø) with 10 ml of water sample,
close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one CALCIO H No. 1 tablet straight from the
foil to the 10 ml water sample, crush the tablet using
a clean stirring rod and dissolve the tablet completely.
6. Add one CALCIO H No. 2 tablet straight from the foil
to the same water sample and crush the tablet using a
clean stirring rod.
7. Close the vial tightly with the cap and swirl gently several
times until the tablet is completely dissolved.
8. Place the vial in the sample chamber making sure that
the
marks are aligned.
9. Press TEST key.
Wait for a reaction period of 2 minutes.
After the reaction period is finished the measurement
starts automatically.
The result is shown in the display as Calcium
Hardness
.
Zero accepted
prepare Test
press TEST
prepare Zero
press ZERO
Countdown
2:00
1
9 1
89
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. To optimize the readings an optional batch related calibration can be performed using
Mode 40, see page 231.
2. Strong alkaline or acidic water samples must be adjusted to a pH-value between
pH 4 and 10 before the tablets are added (use 1 mol/l Hydrochloride acid resp.
1 mol/l Sodium hydroxide).
3. For accurate test results exactly 10 ml of water sample must be taken for the test.
4. This method was developed from a volumetric procedure for the determination of
Calcium Hardness. Due to undefined conditions, the deviations from the standardised
method may be greater.
5. The tolerance of the method is increasing with higher concentrations. When diluting
samples, this should be taken in account, always measuring in the first third of the
range.
6. Interferences:
•Magnesiumhardnessupto200mg/lCaCO3 does not interfere.
•Ironconcentrationabove10mg/lmaycauselowresults.
•Zincconcentrationabove5mg/lmaycausehighresults.
7.
CaCO
3
°dH
°eH
°fH
°aH
90
MC500 Colorimeter 06-2010
1.1 Methods
Hardness, total
with tablet reagent
2 – 50 mg/l CaCO
3
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample, close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one HARDCHECK P tablet straight from the foil
to the water sample and crush the tablet using a clean
stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
Wait for a reaction period of 5 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display as total Hardness.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
5:00
0
0
2
91
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Strong alkaline or acidic water samples must be adjusted between pH 4 and pH 10
before the tablet is added (use 1 mol/l Hydrochloric acid resp. 1mol/l Sodium hydroxide).
2. Conversion table:
mg/l CaCO
3
°dH °fH °eH
1 mg/l CaCO
3
---- 0.056 0.10 0.07
1 °dH 17.8 ---- 1.78 1.25
1 °fH 10.0 0.56 ---- 0.70
1 °eH 14.3 0.80 1.43 ----
3.
CaCO
3
°dH
°eH
°fH
°aH
92
MC500 Colorimeter 06-2010
1.1 Methods
Hardness, total HR
with Tablet
20 – 500 mg/l CaCO
3
1. Fill a clean vial (24 mm Ø) with 1 ml of the water sam-
ple and 9 ml of deionized water, close tightly with the
cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add one HARDCHECK P tablet straight from the foil
to the water sample and crush the tablet using a clean
stirring rod.
6. Close the vial tightly with the cap and swirl several times
until the tablet is dissolved.
7. Place the vial in the sample chamber making sure that
the
marks are aligned.
8. Press TEST key.
Wait for a reaction period of 5 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display as total Hardness.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
5:00
0
1
2
93
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Strong alkaline or acidic water samples must be adjusted between pH 4 and pH 10
before the tablet is added (use 1 mol/l Hydrochloric acid resp. 1mol/l Sodium hydroxide).
2. Conversion table:
mg/l CaCO
3
°dH °fH °eH
1 mg/l CaCO
3
---- 0.056 0.10 0.07
1 °dH 17.8 ---- 1.78 1.25
1 °fH 10.0 0.56 ---- 0.70
1 °eH 14.3 0.80 1.43 ----
3.
CaCO
3
°dH
°eH
°fH
°aH
94
MC500 Colorimeter 06-2010
1.1 Methods
Hydrazine
with powder reagent
0.05 – 0.5 mg/l N2H4 / 50 – 500 µg/l N2H
4
1. Fill a clean vial (24 mm Ø) with 10 ml of the water
sample (Note 1, 2), close tightly with the cap.
2. Place the vial in the sample chamber making sure that
the
marks are aligned.
3. Press ZERO key.
4. Remove the vial from the sample chamber.
5. Add 1 g HYDRAZINE test powder (Note 3) to the
water sample.
6. Close the vial tightly with the cap and swirl several times
to mix the contents.
7. Press [
] key.
Wait for a reaction period of 10 minutes.
After the reaction period is nished proceed as follows:
8. The slight turbidity that occurs when the reagent is added
must be removed by ltration (Note 4).
9. Place the vial in the sample chamber making sure that
the
marks are aligned.
10. Press TEST key.
The result is shown in the display as Hydrazine.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
10:00
start:
0
5
2
95
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. If the water sample is cloudy, it should be ltered it before performing the zero calibration.
2. The temperature of the water sample should not exceed 21°C.
3. Using the Hydrazine spoon: 1 g is equivalent to one level spoon.
4. Qualitative folded lter papers for medium precipitates are recommended.
5. In order to check whether the reagent has aged (if it has been stored for a lengthy period),
perform the test as described above using tap water. If the result is above the
detection limit of 0.05 mg/l, you should use new reagents, as it could result in major result
deviations.
6. There is an option to change the unit from mg/l to μg/l.
mg/l
µg/l
96
MC500 Colorimeter 06-2010
1.1 Methods
Hydrazine
with Liquid Reagent
0.005 – 0.6 mg/l N2H4 / 5 – 600 µg/l N2H
4
Use two clean vials (24 mm Ø) and mark one as blank for
zeroing.
1. Fill a clean vial with 10 ml deionized water (this is the
blank).
2. Add 1 ml Hydra 2 Rgt Solution into the vial (Note 3).
3. Close the vial tightly with the cap and swirl several times
to mix the contents.
4. Place the vial (the blank) in the sample chamber making
sure that the
marks are aligned.
5. Press ZERO key.
6. Remove the vial from the sample chamber.
7. Fill the second clean vial with 10 ml of the water sample
(this is the sample).
8. Add 1 ml Hydra 2 Rgt Solution into the vial.
9. Close the vial tightly with the cap and swirl several times
to mix the contents.
10. Place the vial (the blank) in the sample chamber making
sure that the
marks are aligned.
11. Press TEST key.
Wait for a reaction period of 12 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display as Hydrazine.
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
Countdown
12:00
0
6
2
97
MC500 Colorimeter 06-2010
1.1 Methods
Notes:
1. Samples cannot be preserved and must be analyzed immediately.
2. Sample temperature should be 21°C ± 4 °C.
3. Caused by the reagent itself the blank may develop a faint yellow color.
4. Interferences:
• Ammonium causes no interferences up to 10 mg/l.
At a concentration of 20 mg/l it is possible. that the test result increase up to 20 %.
• Morpholine does not interfere up to 10 mg/l.
• Highly colored or turbid samples:
Mix 1 part deionized water with 1 part household bleach. Add 1 drop of this
mixture into 25 ml water sample and mix. Use 10 ml prepared sample in place of
deionized water in point 1.
Note: at point 7 use the unprepared water sample.
Principle: Hydrazine is oxidized by the household bleach. The interference by color
will be eliminated by zeroing.
5. There is an option to change the unit from mg/L to μg/L.
mg/l
µg/l
98
MC500 Colorimeter 06-2010
1.1 Methods
Hydrazine
with Vacu-vials
®
K-5003 (see Notes)
0.01 – 0.7 mg/l N2H4 / 10 – 700 µg/l N2H
4
Insert the adapter for 13 mm Ø vials.
1. Place the blank in the sample chamber. The blank is part
of the test kit.
2. Press ZERO key.
3. Remove the blank from the sample chamber.
4. Fill the sample container to the 25 ml mark with the water
sample.
5. Place one Vacu-vial
®
in the sample container. Snap the
tip by pressing the vial against the side of the sample
container. The Vacu-vial® breaks at the neck and the vial
lls automatically. A small volume of inert gas remains in
the Vacu-vial®.
6. Mix the contents of the Vacu-vial
®
by inverting it several
times, allowing the bubble to move from one end to the
other. Dry the outside of the vial.
7. Place the Vacu-vial
®
in the sample chamber.
8. Press TEST key.
Wait for a reaction period of 10 minutes.
After the reaction period is nished the measurement
starts automatically.
The result is shown in the display as Hydrazine.
Countdown
10:00
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
0
7
2
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