OPTIKA
MICROSCOPES
®
ITALY
OPTIKA CAMERA ADAPTERS
Connection Guide
OPTIKA CAMERA ADAPTERS
Table of contents
1. The selection of the C-mount adapter p. 3
1.1 Selecting the C-mount adapter p. 3
1.2 Percentage of eld framed according to eyepieces p. 5
1.3 How to calculate the nal magnication on the
monitor p. 6
2. C-mount cameras p. 7
2.1
Connect a C-mount camera to a biological microscope
2.2 Connect a C-mount camera to a stereomicroscope p. 8
2.3
Adjusting parfocality between eyepiece and
on-screen images
p. 9
p. 7
3. Eyepiece cameras p. 10
3.1 Connect a C-mount camera to a trinocular
microscope p. 10
3.2 Connect a C-mount camera to a monocular/binocular
microscope p. 11
M-620 M-620.1 M-620.2 M-620.3
SLX / SZM / SZN / SZP
ST-090 ST-090.1 ST-090.2
M-114
M-620.3
4. Reex cameras p. 11
4.1 Connect a Reex camera to a trinocular microscope
(using a projection lens) p. 11-12
5. Sensor adapters chart p. 14-15
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M-173
OPTIKA CAMERA ADAPTERS
1 The selection of the C-mount adapter
1.1 Selecting the C-mount adapter
When the users look inside the eyepieces, at a glance they are able to obtain a good perception of what is observed, quickly
evaluating the sample.
When the image is projected to a monitor/screen, the situation is dierent: people is often expecting to have the exact correspondence between what is observed into the eyepieces and what is projected on the screen, but the rule “What You See
Is What You Get” is not always applicable in digital microscopy.
The reason is pretty simple: we are comparing a round image observed in the eyepieces and a rectangular image projected
on the monitor/screen...
Field observed with a 22 mm eyepiece.
Cameras’ sensors are rectangular and the image is a part of what is visible through the eyepieces.
Phisically, it is not possible to carry out the circle squaring, therefore it is absolutely impossible to frame the who le eld observed through the eyepieces, but we have to consider the diagonal of the sensor which varies according to its size.
SENSOR DIAGONAL (mm) LENGHT (mm) HEIGHT (mm)
1/10” 1.60 1.28 0.96
1/8” 2.00 1.60 1.20
1/6” 3.00 2.40 1.80
1/4” 4.00 3.20 2.40
1/3” 6.00 4.80 3.60
1 / 2.3” 7.66 6.17 4.55
1/2” 8.00 6.40 4.80
1 / 1.8” 8.93 7.18 5.32
1 / 1.7” 9.50 7.60 5.70
1 / 1.6” 10.07 8.08 6.01
2/3” 11.00 8.80 6.60
1” 15.86 13.20 8.80
4/3” 21.60 17.30 13
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OPTIKA CAMERA ADAPTERS
1.1 Selecting the C-mount adapter
It is easy to understand that the rectangle and the circle cannot frame the same portion of the image.
F.N. 22mm
8,8 mm
1” chip
13,2 mm
Between microscope and camera it is necessary to put a system, commonly known as “adapter”, that can project on the
sensor the image coming from the microscope, making it available to focus and to monitor projection.
There are several adapters (with various magnication factors) that can enlarge or reduce the microscopic image and make
it available for the camera.
An optimal situation could be to frame all the possible eld using the full dia gonal
of the sensor: in this case, the center of what is observed thorugh the eyepieces
will be visible on the monitor/screen, whilst the side parts of the image are lost.
An alternative solution (but not recommended for the nal eect on the monitor)
is to enlarge the image until the long side of the sensor matches the eld of the
eyepieces.
In this case, the image is “vi gnetting”, generating black corners.
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OPTIKA CAMERA ADAPTERS
1.1 Selecting the C-mount adapter
Basically, C-mount adapters are four: 0.35x, 0.5x, 0.65x, 1x.
These numbers represent the magnication power of each adapter (in terms of %).
0.35x 70%
0.50x 100%
0.65x 132%
1x 200%
Take in consideration that:
• THE HIGHER MAGNIFICATION ON THE SENSOR, THE SMALLER FIELD FRAMED
• THE LOWER MAGNIFICATION ON THE SENSOR, THE BIGGER FIELD FRAMED
1.2 Percentage of eld framed according to eyepieces
Generally, the suggestion is to nd the right compromise between what we would like to observe and what we could achieve
as a nal result.
In order to do that, it is necessary to carefully consider the combination of the camera’s sensor and magnication of the
C-mount adapter to see which part of the eld (also depending on the eld number of the eyepieces) the sensor will be able
to frame.
The table shows the percentage of eld framed when using eyepieces with eld number 22.
SENSOR DIAGONAL (mm) % eld framed
1/10” 1,6 28,6%
1/8” 2 35,8%
1/6” 3 53,7%
1/4” 4 71,6%
1/3” 6 107,4%
1/2.3” 7,66 137,1%
1/2” 8 143,2%
1/1.8” 8,93 159,8%
1/1.7” 9,5 170%
1/1.6” 10,07 180,2%
2/3” 11 196,9%
1” 15,86 283,8%
4/3” 21,6 386,54%
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OPTIKA CAMERA ADAPTERS
1.3 How to calculate the nal magnication on the monitor
Calculating the image magnication on a video monitor can be accomplished rst by determining the optical magnication
and multiplying it by the electronic magnication.
Optical Magnication = Objective Mag. * Projection lens or “C-mount adapter Mag.
The objective magnication is expressed as 10x, 20x etc. and Projection lens or “C”-mount adapter is the optical path magnication that leads to the video camera.
Some typical magnication of the “C”-mount adapter are 0.35X, 0.5X, 0.65X, 0.75X, 1X.
Electronic Magnication = Monitor diagonal size / Camera chip diagonal size
The monitor’s diagonal size is usually expressed as 14”, 15”, 17”, 19”, 20”, 21”,...
The camera’s chip is expressed as 1/3”, 1/2”, 2/3” or 1”.
However, this is not the dimension we use. We need to use the diagonal dimension of the chip.
The table below represents the common chip sizes and their diagonal dimensions in millimetres and inches.
CHIP SIZE DIAGONAL (mm) DIAGONAL (inches)
1/3” 6 0.24
1/2” 8 0.31
2/3” 11 0.43
1” 15,86 0.63
The formula to calculate the nal magnication of the image on the monitor is:
Objective Mag. * Projection lens or “C-mount adapter Mag. * (Monitor diagonal size / Camera chip diagonal size)
Let’s make an example:
We have a microscope where a 2/3” camera with a 0.65 “C”-mount adapter is installed and the image is projected on a 21”
monitor.
Actually we are using 20x objective
Microscope magnication: 20X (objective) * 10X (eyepiece) = 200X
Camera magnication: 20X (objective) * 0.65X (“C”-mount) * 21” (monitor) / 0.43” (chip size) =
20X * 0.65X * 48.83 = 634,79X
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OPTIKA CAMERA ADAPTERS
2 C-mount cameras
2.1 Connect a C-mount camera to a biological microscope
Connect a C-mount camera to a trinocular head of the current OPTIKA microscope range:
• B-380, B-510, B-810, B-1000, IM-3 and IM-5 Series
M-620 M-620.1 M-620.2 M-620.3
M1 M2 M3 M4
M1) Remove camera’s dust
cover
M5
M5) Loose the clamping screw
M9
M2) Remove C-mount adapter
dust cover
M6 M7 M8
M6) Remove trinocular head’s
dust cover
M4 / M5) Connect the C-mount on the camera by screwing rmly
M7 / M8) Install the previously assembled C-mount + camera on
the trinocular head
M9) Tighten the clamping
screw rmly
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OPTIKA CAMERA ADAPTERS
2.2 Connect a C-mount camera to a stereomicroscope
Connect a C-mount camera to a trinocular head of the current OPTIKA stereomicroscope range:
• SLX, SZM, SZO, SZP Series
SLX / SZM / SZO / SZP
ST-090 ST-090.1 ST-090.2 M-620.3
M1 M2 M3 M4
M1) Remove camera’s dust
cover
M5
M5) Loose the clamping screw
M9
M2) Remove C-mount adapter
dust cover
M6 M7 M8
M6) Remove trinocular head’s
dust cover
M4 / M5) Connect the C-mount on the camera by screwing rmly
M7 / M8) Install the previously assembled C-mount + camera on
the trinocular head
M9) Tighten the clamping
screw rmly
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OPTIKA CAMERA ADAPTERS
2.3 Adjusting parfocality between eyepiece and on-screen images
In order to have the same focus when observing the specimen through the eyepieces and on the screen/monitor, please
verify the microscope is properly installed and set and follow the instructions below.
In case of a biological microscope:
1) Use a low power objective and focus the specimen;
2) Switch to the highest dry objective available on the microscope (40x or 60x) and focus the specimen again;
3) Turn on the live-view on the camera, without changing the focus on the microscope;
4) While observing the image on the screen/monitor, adjust the focus by turning the knurled knob on the C-mount adapter
In case of a stereomicroscope:
1) Using a low power magnication and focus the specimen;
2) Reach the highest magnication available using the zoom knob and then focus the specimen again;
3) Turn on the live-view on the camera, without changing the focus on the microscope;
4) While observing the image on the screen/monitor, adjust the focus by turning the knurled knob on the C-mount adapter
The proper parfocality adjustment is obtained when the same focus is reached when looking into the eyepieces and on the
screen/monitor.
F1
ST-090 ST-090.1 ST-090.2
F2 F3
F1 / F2 / F3)
the focus by turning the knurled knob on the C-mount adapter
F4
While observing the image on the screen/monitor, adjust
ST-418 ST-419
F5
M-620 M-620.1 M-620.2
F6
M-620.3
F7
F4 / F5) Loose the clamping knob of the 1x C-mount adapter
F6) While observing the image
on the screen/monitor, adjust
the focus by turning the knurled
knob on the C-mount adapter
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F7) Tighten the clamping knob
on the 1x C-mount adapter
OPTIKA CAMERA ADAPTERS
3 Eyepiece cameras
3.1 Connect a C-mount camera to a trinocular microscope
Connect a camera to a trinocular head of the current OPTIKA microscope and stereomicroscope range using the C-mount
projection lens:
• B-193, B-293
M1
M1) Remove camera’s
dust cover
M2
M2) Remove C-mount
projection lens’ dust
cover
M3
M4
M3 / M4) Connect the C-mount projection lens on
the came ra by screwing rmly
M5
M5) Connect the
C-mount projection lens
on the trinocular port
3.2 Connect a C-mount camera to a monocular/binocular microscope
Connect a camera to a binocular or monocular head of the current OPTIKA microscope and stereomicroscope range using
the C-mount projection lens:
• Any OPTIKA monocular or binocular microscope/stereomicroscope
• Any non-OPTIKA monocular or binocular microscope/stereomicroscope
M1
M2
M3
M4
M5
M1) Remove camera’s
dust cover
M6
M6) Connect the
C-mount projection lens
where the eyepiece has
been removed
M2) Remove C-mount
projection lens’ dust
cover
M7
M7) Properly install the
camera
M3 / M4) Connect the C-mount projection lens on
the came ra by screwing rmly
M8
M9
M8 / M9) For eyepiece sleeves bigger than 23 mm,
use the adapter ring (23 to 30 mm or 23 to 30.5 mm,
according to the sleeve size, both are supplied)
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M5) Remove an eyepiece
OPTIKA CAMERA ADAPTERS
4 Reex cameras
4.1 Connect a Reex camera to a trinocular microscope
Connect a Reex camera to a trinocular head of the current OPTIKA microscope and stereomicroscope range using the
projection lens:
• B-193, B-293
M-173
M1
M1) Screw the Reex adapter to the “T2” ring (provided with the
Reex camera or from its manufacturer)
M2
M3
M2) Connect the Reex camera’s adapter with the “T2” ring
M3) Properly install the Reex camera’s adapter
on the trinocular port
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OPTIKA CAMERA ADAPTERS
4.1 Connect a Reex camera to a trinocular microscope
Connect a Reex camera to a trinocular head of the current OPTIKA microscope and stereomicroscope range using the
projection lens plus the universal adapter:
• B-380, B-510, B-810, B-1000, IM-3, IM-5, SZM, SZN, SZP series
M-173 + M-699
M1
M1) Screw the small ring adapter into the universal adapter M2) Clamp the bottom part of the Reex adapter with the
M3
M2
universal adapter
M4
M3)Screw the Reex adapter to the “T2” ring (provided with the
Reex camera or from its manufacturer)
M4) Follow instructions M5 - M9 described in chapter 2.1
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OPTIKA CAMERA ADAPTERS
Page 13
5. Sensor adapters chart
OPTIKA CAMERA ADAPTERS
Reference
model
SENSOR ADAPTER
1/3”
(1/3” à 1/2,6”)
1/2”
(1/2,5” à 1/1,8”)
Microscope: B-1000BF Sample: micrometer object
Objective: 40x Field to the eyepieces: 530 mm
0,35X
(M-620)
50 mm
0,5X
(M-620.1)
2/3”
1”
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OPTIKA CAMERA ADAPTERS
0,65X
(M-620.2)
1X
(M-620.3)
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v 1.0.0 - OPTIKA reserves the right to make corrections, modications, enhancements, improvements and other changes to its products at any time without notice.
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